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61.
Roney Fontes Guimarães Luciana Rodrigues Camillo Aurizangela Oliveira Sousa Luana Pereira Gonçalves Jamilly Costa Cardoso Macedo Giovano Sousa Siqueira Antônia Marlene Magalhães Barbosa Fátima Cerqueira Alvim Marcio Gilberto Cardoso Costa Carlos Priminho Pirovani 《Tree Genetics & Genomes》2018,14(6):90
Here, we report for the first time the genome-wide identification and expression analysis of the molecular chaperone BiP genes in Citrus. Six genes encoding the conserved protein domain family GPR78/BiP/KAR2 were identified in the genome of Citrus sinensis and C. clementina. Two of them, named here as CsBiP1 and CsBiP2, were classified as true BiPs based on their deduced amino acid sequences. Alignment of the deduced amino acid sequences of CsBiP1 and CsBiP2 with BiP homologs from soybean and Arabidopsis showed that they contain all the conserved functional motifs of BiPs. Analysis of the promoter region of CsBiPs revealed the existence of cis-acting regulatory sequences involved in abiotic, heat-shock, and endoplasmic reticulum (ER) stress responses. Publicly available RNA-seq data indicated that CsBiP1 is abundantly expressed in leaf, flower, fruit, and callus, whereas CsBiP2 expression is rarely detected in any tissues under normal conditions. Comparative quantitative real-time PCR (qPCR) analysis of expression of these genes between C. sinensis grafted on the drought-tolerant “Rangpur” lime (C. limonia) and -sensitive “Flying Dragon” trifoliate orange (Poncirus trifoliata) rootstocks showed that CsBiP1 was upregulated by drought stress on the former but downregulated on the latter, whereas the CsBiP2 mRNA levels were downregulated on drought-stressed “Flying Dragon,” but remained constant on “Rangpur.” CsBiP2 upregulation was only observed in C. sinensis seedlings subjected to osmotic and cold treatments. Taken together, these results indicate the existence of two highly conserved BiP genes in Citrus that are differentially regulated in the different tissues and in response to abiotic stresses. 相似文献
62.
Rodrigo de Almeida Cecília Naomi Nakamura Marina de Lima Fontes Elenice Deffune Sérgio Luis Felisbino Ramon Kaneno 《MABS-AUSTIN》2018,10(1):46-54
Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms: subtractive immunization, monoclonal antibody, tolerization, neonatal, high-zone tolerance, masking immunization. Patent records were also consulted. From the list of results, we included all available reports, from 1985 to present, that used any enhanced immunization technique to produce either polyclonal or monoclonal antibodies. Our examination yielded direct evidence that these enhanced immunization techniques are efficient in obtaining specific antibodies to rare epitopes, with different applications, such as to identify food contaminants or tumor cells. 相似文献
63.
Hyperhydricity in pepper plants regenerated in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects 总被引:1,自引:0,他引:1
M. A. Fontes W. C. Otoni S. M. B. Carolino S. H. Brommonschenkel E. P. B. Fontes M. Fári R. P. Louro 《Plant cell reports》1999,19(1):81-87
Hyperhydricity in regenerated pepper plants was monitored by the induction of the ER-luminal resident protein, as observed
by immunoblotting. Immunoblotting of total protein using an anti-soybean BiP serum indicated that the induction and accumulation
of an 80-kDa protein was related to BiP (Binding protein), a 78-kDa ER-resident molecular chaperone. The anti-BiP serum cross-reacted
with an 80-kDa protein which was significantly induced by hyperhydricity. Based on similar molecular weight and immunological
reactivity we concluded that the 80-kDa protein induced in hyperhydric plants is a BiP homologue. The ultrastructural organisation
of leaves in non-hyperhydric and hyperhydric pepper (Capsicum annuum L.) plants was investigated with the aim of identifying the subcellular changes associated with this phenomenon. In non-hyperhydric
leaves the chloroplasts of the palisade cells had normally developed thylakoids and grana and a low accumulation or absence
of starch grains and plastoglobules. In the hyperhydric plants, however, the chloroplasts exhibited thylakoid disorganisation,
low grana number, an accumulation of large starch grains and a low accumulation or absence of plastoglobules. Although the
structure of mitochondria and peroxisomes did not change in hyperhydric plants, the number of peroxisomes did increase.
Received: 23 July 1998 / Revision received: 26 February 1999 / Accepted: 17 March 1999 相似文献
64.
Luiz A. R. Freitas Lilian V. S. Carvalho Jonathan L. M. Fontes Cassiana S. Souza Reginaldo B. Santos Jr. Lara T. Cardoso Claudio P. Figueira Milton S. Fonseca Neto Rafael C. M. F. Dias Manuela S. Solc Marilda S. Gonalves Setondji C. M. A. Yahouedehou Ceuci L. X. Nunes Geraldo G. S. Oliveira Washington L. C. dosSantos 《Journal of cellular and molecular medicine》2021,25(21):10318
65.
Aureo Banhos Bruno L. Fontes Débora Regina Yogui Mario Henrique Alves Natália Carneiro Ardente Renata Valls Lucas Mendes Barreto Lucas Damásio Átilla Colombo Ferreguetti Andréa Siqueira Carvalho Vitor Roberto Schettino Alexandre Rosa dos Santos Helena Godoy Bergallo Ana Carolina Srbek-Araujo Emilia Patrícia Medici Ariel Canena Arnaud L.J. Desbiez 《Biotropica》2020,52(3):421-426
We report 24 records of giant armadillo roadkill on Brazilian highways in the Cerrado, Pantanal and Amazon biomes illustrating that highways are a threat to this species. However, we also documented the species using underpasses, demonstrating that these structures could help to reduce the risk of roadkill for giant armadillos. 相似文献
66.
67.
Natalia Ritter Ruas Aline Carvalho Pereira Luciana Lopes Silva Pereira Carolina Mesquita Germano Elaine Fontes Ferreira da Cunha Alexandre Alves de Carvalho Osmar Alves Lameira José Eduardo Brasil Pereira Pinto Suzan Kelly Vilela Bertolucci 《化学与生物多样性》2023,20(2):e202200760
This study aimed to quantify verbascoside (VEB), perform molecular docking studies of VEB with the α-glucosidase (GL) of Bacillus stearothermophilus, and evaluate the inhibition of the enzyme by L. dulcis preparations. The substrate concentration and presence of reduced glutathione were evaluated for their effect on the in vitro inhibition of the GL enzyme. Assays were also performed in the presence and absence of simulated gastric fluid. The antidiabetic fractions 2 and 3 were the most inhibited GL, but their activity were significantly decreased in the presence of gastric fluid. Chromatographic analyses confirmed the predominant presence of VEB in the samples. The samples had VEB concentrations between 49.9 and 243.5 mg/g. Simulation of the molecular docking of VEB were consistent with its GL-inhibitory activity. It can conclude that the crude ethanol extract and fractions show inhibitory activity against the GL enzyme. 相似文献
68.
Guilherme H.M. Salvador Rafael J. Borges Bruno Lomonte Matthew R. Lewin Marcos R.M. Fontes 《Biochimica et Biophysica Acta (BBA)/General Subjects》2021,1865(7):129913
BackgroundThe treatment for snakebites is early administration of antivenom, which can be highly effective in inhibiting the systemic effects of snake venoms, but is less effective in the treatment of extra-circulatory and local effects. To complement standard-of-care treatments such as antibody-based antivenoms, natural and synthetic small molecules have been proposed for the inhibition of key venom components such as phospholipase A2 (PLA2) and PLA2-like toxins. Varespladib (compound LY315920) is a synthetic molecule developed and clinically tested aiming to block inflammatory cascades of several diseases associated with high PLA2s. Recent studies have demonstrated this molecule is able to potently inhibit snake venom catalytic PLA2 and PLA2-like toxins.MethodsIn vivo and in vitro techniques were used to evaluate the inhibitory effect of varespladib against MjTX-I. X-ray crystallography was used to reveal details of the interaction between these molecules. A new methodology that combines crystallography, mass spectroscopy and phylogenetic data was used to review its primary sequence.ResultsVarespladib was able to inhibit the myotoxic and cytotoxic effects of MjTX-I. Structural analysis revealed a particular inhibitory mechanism of MjTX-I when compared to other PLA2-like myotoxin, presenting an oligomeric-independent function.ConclusionResults suggest the effectiveness of varespladib for the inhibition of MjTX-I, in similarity with other PLA2 and PLA2-like toxins.General significanceVarespladib appears to be a promissory molecule in the treatment of local effects led by PLA2 and PLA2-like toxins (oligomeric dependent and independent), indicating that this is a multifunctional or broadly specific inhibitor for different toxins within this superfamily. 相似文献
69.
Bellay Sybelle de Oliveira Edson Fontes Almeida-Neto Mário Takemoto Ricardo Massato 《Hydrobiologia》2020,847(13):2873-2882
Hydrobiologia - The extinction of fish species can direct and indirectly affect many groups of associated species, among which parasite communities can be the most susceptible. However, the... 相似文献
70.
Institutional responsibilities in contamination control in research animals and occupational health and safety for animal handlers 总被引:1,自引:0,他引:1
Fontes B 《ILAR journal / National Research Council, Institute of Laboratory Animal Resources》2008,49(3):326-337
The mechanisms for controlling microbial contamination in research animals are similar to those for preventing exposure among animal handlers to naturally occurring pathogens, research-related biohazards, or animal allergens. Research and resource preservation are the primary goals of each approach, and an appropriate assessment of risk is their foundation. The identification of potential risks enables the implementation of relevant risk management or control measures. This article summarizes the components of an occupational health and safety program for animal handlers, including screening, training, work practices, effective use of engineering controls, selection and use of personal protective equipment, and emergency response protocols. The features of a risk assessment and risk management program and the level of interaction and training required to implement and sustain the program correlate well with programs designed to control microbial contamination in laboratory animals. This article includes an explanation of the five Ps of risk assessment and risk management. Pathogens and the proposed experimental procedures account for the first two Ps; the other three focus on training and awareness of the personnel involved in the experiment, protective equipment and work practices, and factors associated with the place (or facility) where the research will be conducted. Animal handlers comprehension and knowledge determine the success of any containment program, and so this review also includes a discussion of critical teaching points for animal handlers and of the importance of evaluating personnel to verify their proficiency and competence in required protocols. 相似文献