全文获取类型
收费全文 | 4750篇 |
免费 | 370篇 |
国内免费 | 2篇 |
出版年
2023年 | 20篇 |
2022年 | 41篇 |
2021年 | 67篇 |
2020年 | 55篇 |
2019年 | 57篇 |
2018年 | 65篇 |
2017年 | 81篇 |
2016年 | 106篇 |
2015年 | 205篇 |
2014年 | 204篇 |
2013年 | 296篇 |
2012年 | 373篇 |
2011年 | 392篇 |
2010年 | 254篇 |
2009年 | 221篇 |
2008年 | 324篇 |
2007年 | 263篇 |
2006年 | 279篇 |
2005年 | 248篇 |
2004年 | 244篇 |
2003年 | 251篇 |
2002年 | 228篇 |
2001年 | 68篇 |
2000年 | 62篇 |
1999年 | 75篇 |
1998年 | 59篇 |
1997年 | 41篇 |
1996年 | 53篇 |
1995年 | 47篇 |
1994年 | 37篇 |
1993年 | 23篇 |
1992年 | 47篇 |
1991年 | 24篇 |
1990年 | 33篇 |
1989年 | 26篇 |
1988年 | 25篇 |
1987年 | 22篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 18篇 |
1983年 | 18篇 |
1982年 | 11篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1977年 | 18篇 |
1976年 | 9篇 |
1975年 | 9篇 |
1974年 | 12篇 |
1973年 | 10篇 |
排序方式: 共有5122条查询结果,搜索用时 15 毫秒
91.
Yann Thierry Le Guen Tony Le Gall Patrick Midoux Philippe Gugan Serge Braun Tristan Montier 《The journal of gene medicine》2020,22(2)
Hydrodynamic limb vein injection is an in vivo locoregional gene delivery method. It consists of administrating a large volume of solution containing nucleic acid constructs in a limb with both blood inflow and outflow temporarily blocked using a tourniquet. The fast, high pressure delivery allows the musculature of the whole limb to be reached. The skeletal muscle is a tissue of choice for a variety of gene transfer applications, including gene therapy for Duchenne muscular dystrophy or other myopathies, as well as for the production of antibodies or other proteins with broad therapeutic effects. Hydrodynamic limb vein delivery has been evaluated with success in a large range of animal models. It has also proven to be safe and well‐tolerated in muscular dystrophy patients, thus supporting its translation to the clinic. However, some possible limitations may occur at different steps of the delivery process. Here, we have highlighted the interests, bottlenecks and potential improvements that could further optimize non‐viral gene transfer following hydrodynamic limb vein injection. 相似文献
92.
Roque d’orbcastel Emmanuelle Bettarel Yvan Dellinger Marion Sadoul Bastien Bouvier Thierry Amandé Justin Monin Dagorn Laurent Geffroy Benjamin 《Environmental Biology of Fishes》2021,104(6):725-732
Environmental Biology of Fishes - Cortisol is recognized as a physiological indicator of stress in fish. However, this hormone is typically measured in plasma samples. In this study, cortisol... 相似文献
93.
Bobby G. Ng Paulina Sosicka François Fenaille Annie Harroche Sandrine Vuillaumier-Barrot Mindy Porterfield Zhi-Jie Xia Shannon Wagner Michael J. Bamshad Marie-Christine Vergnes-Boiteux Sophie Cholet Stephen Dalton Anne Dell Thierry Dupré Mathieu Fiore Stuart M. Haslam Yohann Huguenin Tadahiro Kumagai Hudson H. Freeze 《American journal of human genetics》2021,108(6):1040-1052
94.
Tonna O. Mojekwu Michael J. Cunningham Roger I. Bills Petrus C. Pretorius Thierry B. Hoareau 《Journal of fish biology》2021,98(2):498-506
The importance of Oreochromis in worldwide aquaculture and regional fisheries motivates the study of their genetic diversity in their native range. In this article, all mitochondrial cytochrome c oxidase subunit I gene (COI) sequences of Oreochromis species are retrieved from Barcode of Life Data system to quantify the available DNA barcoding information from wild individuals collected within the native ranges of the respective species. It is found that 70% of the known species in the genus still lack a COI barcode, and only 15% of the available sequences are from within the respective native ranges. Many of the available sequences have been produced from specimens acquired from aquaculture and introduced, naturalized populations, making the assessment of variation within the original native range challenging. Analyses of the wild-collected fraction of available sequences indicated the presence of cryptic lineages within Nile tilapia Oreochromis niloticus and O. schwebischi, the occurrence of potential introgressive hybridization between O. niloticus and blue tilapia O. aureus, and potential ancestral polymorphism between Karonga tilapia O. karongae and black tilapia O. placidus. This article also reports a case of misidentification of O. mweruensis as longfin tilapia O. macrochir. These results stress the importance of improving the knowledge of genetic variation within the native ranges of Oreochromis species for better-informed conservation of these natural resources. 相似文献
95.
Hlne Bouvrais Laurent Chesneau Yann Le Cunff Danielle Fairbrass Nina Soler Sylvain Pastezeur Thierry Pcot Charles Kervrann Jacques Pcraux 《EMBO reports》2021,22(5)
In Caenorhabditis elegans zygote, astral microtubules generate forces essential to position the mitotic spindle, by pushing against and pulling from the cortex. Measuring microtubule dynamics there, we revealed the presence of two populations, corresponding to pulling and pushing events. It offers a unique opportunity to study, under physiological conditions, the variations of both spindle‐positioning forces along space and time. We propose a threefold control of pulling force, by polarity, spindle position and mitotic progression. We showed that the sole anteroposterior asymmetry in dynein on‐rate, encoding pulling force imbalance, is sufficient to cause posterior spindle displacement. The positional regulation, reflecting the number of microtubule contacts in the posterior‐most region, reinforces this imbalance only in late anaphase. Furthermore, we exhibited the first direct proof that dynein processivity increases along mitosis. It reflects the temporal control of pulling forces, which strengthens at anaphase onset following mitotic progression and independently from chromatid separation. In contrast, the pushing force remains constant and symmetric and contributes to maintaining the spindle at the cell centre during metaphase. 相似文献
96.
97.
98.
Taiki Hanzawa Kyohei Shibasaki Takahiro Numata Yukio Kawamura Thierry Gaude Abidur Rahman 《The Plant cell》2013,25(9):3424-3433
High-temperature-mediated adaptation in plant architecture is linked to the increased synthesis of the phytohormone auxin, which alters cellular auxin homeostasis. The auxin gradient, modulated by cellular auxin homeostasis, plays an important role in regulating the developmental fate of plant organs. Although the signaling mechanism that integrates auxin and high temperature is relatively well understood, the cellular auxin homeostasis mechanism under high temperature is largely unknown. Using the Arabidopsis thaliana root as a model, we demonstrate that under high temperature, roots counterbalance the elevated level of intracellular auxin by promoting shootward auxin efflux in a PIN-FORMED2 (PIN2)-dependent manner. Further analyses revealed that high temperature selectively promotes the retrieval of PIN2 from late endosomes and sorts them to the plasma membrane through an endosomal trafficking pathway dependent on SORTING NEXIN1. Our results demonstrate that recycling endosomal pathway plays an important role in facilitating plants adaptation to increased temperature. 相似文献
99.
Professional workers and consumers frequently use hard-surface cleaning products and these products may contain glycol ethers (GEs), such as 2-Butoxyethanol (2-BE). Governmental agencies have set exposure limits for some chemicals used in cleaning products and these exposure limits have been used as guides to protect human health. The study objectives were to determine realistic inhalation exposures for professional workers performing multiple, sequential cleaning tasks and compare the exposures to the acute reference exposure level (REL), which California established for 2-BE. The ConsExpo model was acceptable for evaluating exposure based on a comparison of its predictions to experimentally measured 2-BE vapor concentrations from hard-surface cleaning. The typical worker exposure was predicted for a cleaning scenario consisting of three bathrooms and three kitchens (or three bedrooms) in a 1-h period. This exposure scenario would not be expected to result in significant health consequences because the predicted exposure was much lower than the REL. The predicted chronic and aggregate exposures were also acceptable. This analysis identified two important variables that affect inhalation exposure: cleaners should be used with adequate ventilation and wet wiping towels should be properly disposed so that they are not a source of continuing exposure. 相似文献
100.
Didier Boturyn Eric Defrancq Veacute; onique Ducros Catherine Fontaine Jean Lhomme 《Nucleosides, nucleotides & nucleic acids》2013,32(10-11):2069-2077
Abstract Reaction of abasic site-containing oligonucleotides with an oxyamino fluorescent label is described. The reaction represents an efficient method to functionalize oligonucleotides at preselected positions. 相似文献