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排序方式: 共有124条查询结果,搜索用时 15 毫秒
61.
Dmitry V. Semenov Alexander S. Fomin Elena V. Kuligina Olga A. Koval Vera A. Matveeva Irina N. Babkina Nina V. Tikunova Vladimir A. Richter 《The protein journal》2010,29(3):174-180
We recently isolated and characterized a human milk peptide, lactaptin, which induced apoptosis of cultured human MCF-7 cells.
Lactaptin was identified as a proteolytic fragment of human kappa-casein. Here, we generated two recombinant analogs of the
peptide, RL1 and RL2, containing truncated and complete amino acid sequences of lactaptin, respectively. Analogs were produced
in E.coli, purified and assayed for biological activity on cultured human MCF-7 cells. RL1 was shown to induce only a small decrease
in cell viability, whereas RL2 lowered the viability of MCF-7 cells by 60%. This reduction in MCF-7 cell viability was associated
with apoptosis, which was indicated by phosphatidilserine externalization and caspase-7 activation. The viability of A549
and Hep-2 cells was also reduced by RL2, albeit to a lesser degree than seen with MCF-7 cells; this reduced viability was
not accompanied by apoptosis. Non-malignant human mesenchymal stem cells (MSC) were completely resistant to RL2 action. 相似文献
62.
63.
S. V. Fomin S. D. Ryazanov I. A. Usatov V. N. Burkanov 《Russian Journal of Marine Biology》2017,43(3):245-248
The biology of the Cuvier’s beaked whale, Ziphius cavirostris Cuvier, 1823, in Russian waters remains almost unstudied. The species is encountered rarely elsewhere in the seas of Russia, except for the waters off the Commander Islands. Currently, some researchers express doubt about the accuracy of the known Cuvier’s beaked whale records in the region. This report provides information on the confirmed encounters with Cuvier’s beaked whale off the Commander Islands and eastern Kamchatka. 相似文献
64.
O. I. Guliy B. D. Zaitsev A. M. Shikhabudinov A. A. Teplykh I. A. Borodina S. A. Pavliy O. S. Larionova A. S. Fomin S. A. Staroverov L. A. Dykman O. V. Ignatov 《Applied Biochemistry and Microbiology》2016,52(4):457-463
It has been demonstrated that electroacoustic analysis with polyclonal antibodies can be used for bacteriophage detection. The frequency dependences of the real and imaginary parts of electrical impedance of a resonator with a viral suspension with antibodies were shown to be essentially different from the dependences of a resonator with control viral suspension without antibodies. It was shown that ΦAl-Sp59b bacteriophages were detected with the use of antibodies in the presence of foreign virus particles. The ΦAl-Sp59b bacteriophage content in the analyzed suspension was ~1010–106 phages/mL; the time of analysis was no more than 5 min. The optimally informative parameter for obtaining reliable information was the change in the real or imaginary part of electrical impedance at a fixed frequency near the resonance upon the addition of specific antibodies to the analyzed suspension. It was demonstrated that the interaction between bacteriophages and antibodies can be recorded, offering good prospects for the development of a biological sensor for liquid-phase identification and virus detection. 相似文献
65.
Some Bacillus subtilis strains demonstrate a high degree of pHV14 and pP1251 plasmid integration into the chromosome (1.10(-1]. According to the data of genetical analysis the plasmids are integrated into several regions of the same chromosome. A significant plasmid amplification in the composition of chromosome is observed when breeding on an increasing chloramphenicol concentration (20-300 micrograms/ml). Both tandem repetitions and single plasmid DNA copies and their fragments are found in the bacterial chromosomal DNA. 相似文献
66.
Usatyuk PV Fomin VP Shi S Garcia JG Schaphorst K Natarajan V 《American journal of physiology. Lung cellular and molecular physiology》2003,285(5):L1006-L1017
Diperoxovanadate (DPV), a potent inhibitor of protein tyrosine phosphatases and activator of tyrosine kinases, alters endothelial barrier function via signaling pathways that are incompletely understood. One potential pathway is Src kinase-mediated tyrosine phosphorylation of proteins such as cortactin that regulate endothelial cell (EC) cytoskeleton assembly. As DPV modulates endothelial cell signaling via protein tyrosine phosphorylation, we determined the role of DPV-induced intracellular free calcium concentration ([Ca2+]i) in activation of Src kinase, cytoskeletal remodeling, and barrier function in bovine pulmonary artery endothelial cells (BPAECs). DPV in a dose- and time-dependent fashion increased [Ca2+]i, which was partially blocked by the calcium channel blockers nifedipine and Gd3+. Treatment of cells with thapsigargin released Ca2+ from the endoplasmic reticulum, and subsequent addition of DPV caused no further change in [Ca2+]i. These data suggest that DPV-induced [Ca2+]i includes Ca release from the endoplasmic reticulum and Ca influx through store-operated calcium entry. Furthermore, DPV induced an increase in protein tyrosine phosphorylation, phosphorylation of Src and cortactin, actin remodeling, and altered transendothelial electrical resistance in BPAECs. These DPV-mediated effects were significantly attenuated by BAPTA (25 microM), a chelator of [Ca2+]i. Immunofluorescence studies reveal that the DPV-mediated colocalization of cortactin with peripheral actin was also prevented by BAPTA. Chelation of extracellular Ca2+ by EGTA had marginal effects on DPV-induced phosphorylation of Src and cortactin; actin stress fibers formation, however, affected EC barrier function. These data suggest that DPV-induced changes in [Ca2+]i regulate endothelial barrier function using signaling pathways that involve Src and cytoskeleton remodeling. 相似文献
67.
68.
A. S. Fomin O. A. Koval D. V. Semenov M. O. Potapenko E. V. Kuligina Yu. Ya. Kit V. A. Richter 《Russian Journal of Bioorganic Chemistry》2012,38(1):77-82
Earlier, a pro-apoptotic peptide lactaptin was isolated from human milk and characterized and its recombinant analogue RL2
was generated. Both peptides were demonstrated to be capable of apoptotic cell death induction in human breast adenocarcinoma
MCF-7 cells. In the work, biochemical markers of RL2-induced MCF-7 apoptosis are analyzed. Activation of initiator and effector
caspases, as well as apoptotic changes in mitochondrial membrane potential and cytoplasm membrane composition in cells treated
with RL2, were analyzed using flow cytometry and Western blot techniques. MCF-7 cell death induced by RL2 was found to be
associated with phosphatidylserine exposure on the surface of the plasma membrane. Also, RL2 was demonstrated to induce dissipation
of the mitochondrial membrane potential and activation of initiator caspases 8 and 9 and an effector caspase 7. 相似文献
69.
Fomin V. V. Shavnin S. A. Marina N. V. Novoselova G. N. 《Russian Journal of Plant Physiology》2001,48(5):657-661
The pigment composition and light-response curves of the indices of prompt chlorophyll fluorescence were investigated in needles sampled from various parts of the tree crown in pine trees growing under natural conditions at various distances from a source of industrial air pollution (a copper-melting works). Shading and pollution decreased the threshold light level of the state corresponding to the second phase of the light-response curve of nonphotochemical chlorophyll fluorescence quenching, increased a nonradiating dissipation of the absorbed light energy, and decreased the rate of electron transfer between PSII and PSI. The patterns thus established demonstrate the existence of nonspecific chloroplast responses to pollution and shading similar in their mechanism, which decrease the efficiency of light energy utilization. 相似文献
70.