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Andrea Raimondi Giorgia Girotti Gian Andrea Blengini Debora Fino 《The International Journal of Life Cycle Assessment》2012,17(8):987-996
Purpose
While the application of Life Cycle Assessment (LCA) to lubricants can be considered fully operational for general purposes outside the lubricants industry, where Life Cycle Inventories (LCIs) of mineral and synthetic base oils can be used interchangeably and where additives can be excluded, this is not the case for research and development purposes within the industry. Previous LCAs of base oils are not sufficiently detailed and comprehensive for R&D purposes, and there are no LCAs of lube additives and fully formulated lubricants. The aim of this paper is to integrate and expand previous LCAs of base oils and to investigate on the contribution of lube additives to the environmental impacts of a fully formulated lubricant.Materials and methods
This study considers three base oils (mineral, poly-alpha-olefins (PAO) and hydrocracked) and a set of lubricating additives typically used in fully formulated engine oil. The LCA model is based on both industry and literature data.Results and discussion
Trends in the lubricants industry towards more sophisticated base oils correspond to remarkably higher environmental impacts per kilogram of product but lead to reduced impacts per kilometre. The contribution of additives to the life cycle impacts of commercial lube oil was found to be remarkably high for some impact categories (nearly 35?% for global warming).Conclusions
As base oil is concerned, this study made the point on data availability and provided a contribution in order to integrate and expand previous LCAs of mineral base oil and PAO. On the side of additives, the main conclusion is that in modern lubricants, the contribution of additives in terms of environmental impact can be remarkably high and, therefore, they should not be excluded. 相似文献535.
We show that amino acid covariation in proteins, extracted from the evolutionary sequence record, can be used to fold transmembrane proteins. We use this technique to predict previously unknown 3D structures for 11 transmembrane proteins (with up to 14 helices) from their sequences alone. The prediction method (EVfold_membrane) applies a maximum entropy approach to infer evolutionary covariation in pairs of sequence positions within a protein family and then generates all-atom models with the derived pairwise distance constraints. We benchmark the approach with blinded de novo computation of known transmembrane protein structures from 23 families, demonstrating unprecedented accuracy of the method for large transmembrane proteins. We show how the method can predict oligomerization, functional sites, and conformational changes in transmembrane proteins. With the rapid rise in large-scale sequencing, more accurate and more comprehensive information on evolutionary constraints can be decoded from genetic variation, greatly expanding the repertoire of transmembrane proteins amenable to modeling by this method. 相似文献
536.
Matthews DC Brillant MG Clovis JB McNally ME Filiaggi MJ Kotzer RD Lawrence HP 《Gerodontology》2012,29(2):e656-e666
doi: 10.1111/j.1741‐2358.2011.00540.x Assessing the oral health of an ageing population: methods, challenges and predictors of survey participation Objectives: To examine predictors of participation and to describe the methodological considerations of conducting a two‐stage population‐based oral health survey. Methods: An observational, cross‐sectional survey (telephone interview and clinical oral examination) of community‐dwelling adults aged 45–64 and ≥65 living in Nova Scotia, Canada was conducted. Results: The survey response rate was 21% for the interview and 13.5% for the examination. A total of 1141 participants completed one or both components of the survey. Both age groups had higher levels of education than the target population; the age 45–64 sample also had a higher proportion of females and lower levels of employment than the target population. Completers (participants who completed interview and examination) were compared with partial completers (who completed only the interview), and stepwise logistic regression was performed to examine predictors of completion. Identified predictors were as follows: not working, post‐secondary education and frequent dental visits. Conclusion: Recruitment, communications and logistics present challenges in conducting a province‐wide survey. Identification of employment, education and dental visit frequency as predictors of survey participation provide insight into possible non‐response bias and suggest potential for underestimation of oral disease prevalence in this and similar surveys. This potential must be considered in analysis and in future recruitment strategies. 相似文献
537.
Besserer GM Nicoll DA Abramson J Philipson KD 《The Journal of biological chemistry》2012,287(11):8652-8659
The superfamily of cation/Ca(2+) exchangers includes both Na(+)/Ca(2+) exchangers (NCXs) and Na(+)/Ca(2+),K(+) exchangers (NCKX) as the families characterized in most detail. These Ca(2+) transporters have prominent physiological roles. For example, NCX and NCKX are important in regulation of cardiac contractility and visual processes, respectively. The superfamily also has a large number of members of the YrbG family expressed in prokaryotes. However, no members of this family have been functionally expressed, and their transport properties are unknown. We have expressed, purified, and characterized a member of the YrbG family, MaX1 from Methanosarcina acetivorans. MaX1 catalyzes Ca(2+) uptake into membrane vesicles. The Ca(2+) uptake requires intravesicular Na(+) and is stimulated by an inside positive membrane potential. Despite very limited sequence similarity, MaX1 is a Na(+)/Ca(2+) exchanger with kinetic properties similar to those of NCX. The availability of a prokaryotic Na(+)/Ca(2+) exchanger should facilitate structural and mechanistic investigations. 相似文献
538.
Inga Bauer Alessia Grozio Denise Lasigliè Giovanna Basile Laura Sturla Mirko Magnone Giovanna Sociali Debora Soncini Irene Caffa Alessandro Poggi Gabriele Zoppoli Michele Cea Georg Feldmann Raul Mostoslavsky Alberto Ballestrero Franco Patrone Santina Bruzzone Alessio Nencioni 《The Journal of biological chemistry》2012,287(49):40924-40937
539.
Estefania P. C. Azevedo Anderson B. Guimar?es-Costa Guilherme S. Torezani Carolina A. Braga Fernando L. Palhano Jeffery W. Kelly Elvira M. Saraiva Debora Foguel 《The Journal of biological chemistry》2012,287(44):37206-37218
The accumulation of amyloid fibrils is a feature of amyloid diseases, where cell toxicity is due to soluble oligomeric species that precede fibril formation or are formed by fibril fragmentation, but the mechanism(s) of fragmentation is still unclear. Neutrophil-derived elastase and histones were found in amyloid deposits from patients with different systemic amyloidoses. Neutrophil extracellular traps (NETs) are key players in a death mechanism in which neutrophils release DNA traps decorated with proteins such as elastase and histones to entangle pathogens. Here, we asked whether NETs are triggered by amyloid fibrils, reasoning that because proteases are present in NETs, protease digestion of amyloid may generate soluble, cytotoxic species. We show that amyloid fibrils from three different sources (α-synuclein, Sup35, and transthyretin) induced NADPH oxidase-dependent NETs in vitro from human neutrophils. Surprisingly, NET-associated elastase digested amyloid fibrils into short species that were cytotoxic for BHK-21 and HepG2 cells. In tissue sections from patients with primary amyloidosis, we also observed the co-localization of NETs with amyloid deposits as well as with oligomers, which are probably derived from elastase-induced fibril degradation (amyloidolysis). These data reveal that release of NETs, so far described to be elicited by pathogens, can also be triggered by amyloid fibrils. Moreover, the involvement of NETs in amyloidoses might be crucial for the production of toxic species derived from fibril fragmentation. 相似文献
540.
Galvão AS Melo JW Monteiro VB Lima DB De Moraes GJ Gondim MG 《Experimental & applied acarology》2012,57(1):1-13
The dispersal of plant-feeding mites can occur involuntarily, through transportation of infested plant parts, or voluntarily,
by walking to new plant parts or to suitable spots where biotic (phoresis) or abiotic (wind, agricultural tools, etc.) factors
carry them over long distances. Elucidating the dispersal mechanisms of the coconut mite, Aceria guerreronis Keifer, is important for understanding the process of colonization of new fruits of a same or different plants, essential
for the improvement of control strategies of this serious coconut pest. Thus, the objective of this work was to investigate
the voluntary dispersal mechanisms of this mite. The hypothesis that the coconut mite disperses by walking, phoresis or wind
were tested. The coconut mite was shown to be able to walk short distances between fruits of the same bunch or between bunches
of the same plant. Phoresis on insects of the orders Hymenoptera (Apidae), Coleoptera (Curculionidae) and Lepidoptera (Phycitidae)
was evaluated in the laboratory and in the field. Although in the laboratory mites were shown to be able to climb onto honeybees,
field investigations failed to show these insects as important carriers of the pest, corroborating findings of previous works;
however, both laboratory and field investigations suggested the curculionid Parisoschoenus obesulus Casey to be able to transport the coconut mite between plants. Similarly, laboratory and field investigations suggested wind
to be important in the dispersal of the coconut mite between plants. 相似文献