全文获取类型
收费全文 | 3708篇 |
免费 | 297篇 |
出版年
2024年 | 3篇 |
2023年 | 37篇 |
2022年 | 78篇 |
2021年 | 156篇 |
2020年 | 82篇 |
2019年 | 100篇 |
2018年 | 119篇 |
2017年 | 99篇 |
2016年 | 138篇 |
2015年 | 243篇 |
2014年 | 260篇 |
2013年 | 267篇 |
2012年 | 441篇 |
2011年 | 360篇 |
2010年 | 208篇 |
2009年 | 179篇 |
2008年 | 250篇 |
2007年 | 231篇 |
2006年 | 164篇 |
2005年 | 150篇 |
2004年 | 109篇 |
2003年 | 107篇 |
2002年 | 85篇 |
2001年 | 22篇 |
2000年 | 12篇 |
1999年 | 21篇 |
1998年 | 15篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 7篇 |
1992年 | 7篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1983年 | 2篇 |
1977年 | 1篇 |
1976年 | 3篇 |
1971年 | 1篇 |
1964年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1955年 | 1篇 |
1934年 | 1篇 |
1933年 | 2篇 |
1932年 | 1篇 |
排序方式: 共有4005条查询结果,搜索用时 31 毫秒
61.
Coralie E. Salesse‐Smith Robert E. Sharwood Florian A. Busch David B. Stern 《Plant biotechnology journal》2020,18(6):1409-1420
Many C4 plants, including maize, perform poorly under chilling conditions. This phenomenon has been linked in part to decreased Rubisco abundance at lower temperatures. An exception to this is chilling‐tolerant Miscanthus, which is able to maintain Rubisco protein content under such conditions. The goal of this study was to investigate whether increasing Rubisco content in maize could improve performance during or following chilling stress. Here, we demonstrate that transgenic lines overexpressing Rubisco large and small subunits and the Rubisco assembly factor RAF1 (RAF1‐LSSS), which have increased Rubisco content and growth under control conditions, maintain increased Rubisco content and growth during chilling stress. RAF1‐LSSS plants exhibited 12% higher CO2 assimilation relative to nontransgenic controls under control growth conditions, and a 17% differential after 2 weeks of chilling stress, although assimilation rates of all genotypes were ~50% lower in chilling conditions. Chlorophyll fluorescence measurements showed RAF1‐LSSS and WT plants had similar rates of photochemical quenching during chilling, suggesting Rubisco may not be the primary limiting factor that leads to poor performance in maize under chilling conditions. In contrast, RAF1‐LSSS had improved photochemical quenching before and after chilling stress, suggesting that increased Rubisco may help plants recover faster from chilling conditions. Relatively increased leaf area, dry weight and plant height observed before chilling in RAF1‐LSSS were also maintained during chilling. Together, these results demonstrate that an increase in Rubisco content allows maize plants to better cope with chilling stress and also improves their subsequent recovery, yet additional modifications are required to engineer chilling tolerance in maize. 相似文献
62.
Maria Carolina Florian Hanna Leins Michael Gobs Yang Han Gina Marka Karin Soller Angelika Vollmer Vadim Sakk Kalpana J. Nattamai Ahmad Rayes Xueheng Zhao Kenneth Setchell Medhanie Mulaw Wolfgang Wagner Yi Zheng Hartmut Geiger 《Aging cell》2020,19(9)
Cdc42 is a small RhoGTPase regulating multiple functions in eukaryotic cells. The activity of Cdc42 is significantly elevated in several tissues of aged mice, while the Cdc42 gain‐of‐activity mouse model presents with a premature aging‐like phenotype and with decreased lifespan. These data suggest a causal connection between elevated activity of Cdc42, aging, and reduced lifespan. Here, we demonstrate that systemic treatment of aged (75‐week‐old) female C57BL/6 mice with a Cdc42 activity‐specific inhibitor (CASIN) for 4 consecutive days significantly extends average and maximum lifespan. Moreover, aged CASIN‐treated animals displayed a youthful level of the aging‐associated cytokines IL‐1β, IL‐1α, and INFγ in serum and a significantly younger epigenetic clock as based on DNA methylation levels in blood cells. Overall, our data show that systemic administration of CASIN to reduce Cdc42 activity in aged mice extends murine lifespan. 相似文献
63.
Florian M. Rossmann Isabelle Hug Matteo Sangermani Urs Jenal Morgan Beeby 《Molecular microbiology》2020,114(3):443-453
Bacterial flagellar motility is controlled by the binding of CheY proteins to the cytoplasmic switch complex of the flagellar motor, resulting in changes in swimming speed or direction. Despite its importance for motor function, structural information about the interaction between effector proteins and the motor are scarce. To address this gap in knowledge, we used electron cryotomography and subtomogram averaging to visualize such interactions inside Caulobacter crescentus cells. In C. crescentus, several CheY homologs regulate motor function for different aspects of the bacterial lifestyle. We used subtomogram averaging to image binding of the CheY family protein CleD to the cytoplasmic Cring switch complex, the control center of the flagellar motor. This unambiguously confirmed the orientation of the motor switch protein FliM and the binding of a member of the CheY protein family to the outside rim of the C ring. We also uncovered previously unknown structural elaborations of the alphaproteobacterial flagellar motor, including two novel periplasmic ring structures, and the stator ring harboring eleven stator units, adding to our growing catalog of bacterial flagellar diversity. 相似文献
64.
Florian A. Busch 《The Plant journal : for cell and molecular biology》2020,101(4):919-939
Photorespiratory metabolism is essential for plants to maintain functional photosynthesis in an oxygen‐containing environment. Because the oxygenation reaction of Rubisco is followed by the loss of previously fixed carbon, photorespiration is often considered a wasteful process and considerable efforts are aimed at minimizing the negative impact of photorespiration on the plant’s carbon uptake. However, the photorespiratory pathway has also many positive aspects, as it is well integrated within other metabolic processes, such as nitrogen assimilation and C1 metabolism, and it is important for maintaining the redox balance of the plant. The overall effect of photorespiratory carbon loss on the net CO2 fixation of the plant is also strongly influenced by the physiology of the leaf related to CO2 diffusion. This review outlines the distinction between Rubisco oxygenation and photorespiratory CO2 release as a basis to evaluate the costs and benefits of photorespiration. 相似文献
65.
Engelhardt Ilonka C. Niklaus Pascal A. Bizouard Florian Breuil Marie-Christine Rouard Nadine Deau Florence Philippot Laurent Barnard Romain L. 《Plant and Soil》2021,461(1-2):151-162
Plant and Soil - Contrasting nutrient-acquisition strategies would explain why species differ in their distribution in relation to soil phosphorus (P) availability, promoting diversity. However,... 相似文献
66.
Trébouet Florian Malaivijitnond Suchinda Reichard Ulrich H. 《Primates; journal of primatology》2021,62(3):491-505
Primates - Macaque reproductive patterns range from strictly seasonal breeding to non-seasonal breeding, but factors explaining this variation are not fully understood. Valid reproductive... 相似文献
67.
68.
69.
Birthe Tegtmeyer Gabrielle Vieyres Daniel Todt Chris Lauber Corinne Ginkel Michael Engelmann Maike Herrmann Christian K. Pfaller Florian W. R. Vondran Ruth Broering Ehsan Vafadarnejad Antoine-Emmanuel Saliba Christina Puff Wolfgang Baumgrtner Csaba Miskey Zoltn Ivics Eike Steinmann Thomas Pietschmann Richard J. P. Brown 《Journal of virology》2021,95(10)
70.