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Philippe Bussières Nadia Bertin Cindy E. Morris Christian Vigne Patrick Orlando Catherine Glaux Valérie Sévenier Hélène Floret Jean Bernadac Sylvie Korownikoff 《In vitro cellular & developmental biology. Plant》2011,47(6):743-751
The effect of sap sucrose concentration on fruit expansion is poorly understood. We investigated the effects of sucrose concentration
on growth of tomato fruits, detached from the plant after the cell multiplication phase. A semi-open device was built to grow
detached fruits, where the fruit pedicel was immersed in a nutrient solution which was automatically replaced each day. About
22 d after anthesis, tomato fruits were harvested, disinfected in alcohol, and placed in the device with a sterile nutrient
solution containing 5% sucrose. After about 1 wk, the sucrose concentration was either increased to 15% or maintained at 5%.
The fruit expansion rate decreased when the sucrose concentration was increased, and after about 1 wk, these fruits weighed
less than those grown at 5% sucrose. Although all the fruits in the device grew much less than those maintained on the plant,
a high sap sucrose concentration applied to the excised fruits after the cell multiplication phase inhibited the expansion
of tomato fruits. The device and method developed here will be useful for future studies of fruit growth. 相似文献
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