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排序方式: 共有551条查询结果,搜索用时 46 毫秒
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Maria J. Buzon Enrique Martin-Gayo Florencia Pereyra Zhengyu Ouyang Hong Sun Jonathan Z. Li Michael Piovoso Amy Shaw Judith Dalmau Nadine Zangger Javier Martinez-Picado Ryan Zurakowski Xu G. Yu Amalio Telenti Bruce D. Walker Eric S. Rosenberg Mathias Lichterfeld 《Journal of virology》2014,88(17):10056-10065
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Wetlands are subject to invasion by exotic plant species, especially during the dry season when they resemble terrestrial
systems; therefore, terrestrial plants could exploit this situation to colonize this environment. We analyzed P. anserina invading Patagonian wetlands in terms of elemental ratios that would modify wetland stoichiometry due to organic matter inputs.
We studied the elemental relationship (carbon/nitrogen/phosphorus) of P. anserina in comparison with native emergent macrophytes (Eleocharis pachicarpa and Carex aematorrhyncha). These plant species are common and dominant in the wetland. Additionally, we analyzed the presence of mycorrhizal fungi
in the roots and their proportion of root infection. Our study reveals that the invasive species presented nutrient (especially
phosphorus) allocation in roots and differences in mycorrhizal infection, with a predominance of arbuscular mycorrhiza, compared
with native species. During flooded periods with the decay of aerial parts, P. anserina stores phosphorus in the roots and releases dissolved organic matter of high molecular weight molecules, high color, and
a high C-to-nutrient ratio in comparison with native macrophytes. These results show the strategy of an invasive terrestrial
plant in temporary aquatic systems, and how the elemental relationships of the invasive plant can modify the stoichiometry
of the environment. 相似文献
108.
María Florencia Gottardo Matías L. Pidre Camila Zuccato Antonela S. Asad Mercedes Imsen Gabriela Jaita Marianela Candolfi Víctor Romanowski Adriana Seilicovich 《Apoptosis : an international journal on programmed cell death》2018,23(2):143-151
Pituitary tumors are the most common primary intracranial neoplasms. Humanin (HN) and Rattin (HNr), a rat homolog of HN, are short peptides with a cytoprotective action. In the present study, we aimed to evaluate whether endogenous HNr plays an antiapoptotic role in pituitary tumor cells. Thus, we used RNA interference based on short-hairpin RNA (shRNA) targeted to HNr (shHNr). A plasmid including the coding sequences for shHNr and dTomato fluorescent reporter gene was developed (pUC-shHNr). Transfection of somatolactotrope GH3 cells with pUC-shHNr increased apoptosis, suggesting that endogenous HNr plays a cytoprotective role in pituitary tumor cells. In order to evaluate the effect of blockade of endogenous HNr expression in vivo, we constructed a recombinant baculovirus (BV) encoding shHNr (BV-shHNr). In vitro, BV-shRNA was capable of transducing more than 80% of GH3 cells and decreased HNr mRNA. Also, BV-shHNr increased apoptosis in transduced GH3 cells. Intratumor injection of BV-shHNr to nude mice bearing s.c. GH3 tumors increased the number of apoptotic cells, delayed tumor growth and enhanced survival rate, suggesting that endogenous HNr may be involved in pituitary tumor progression. These preclinical data suggests that the silencing of HN expression could have a therapeutic impact on the treatment of pituitary tumors. 相似文献
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Florencia?C.?Cardoso Susana?Goncalves Pablo?G.?Mele Natalia?C.?Liria Leonardo?Sganga Ignacio?Diaz Perez Ernesto?J.?Podesta Angela?R.?SolanoEmail authorView authors OrcID profile 《Human genomics》2018,12(1):39
Background
Ovarian cancer is the leading cause of death worldwide among gynecologic malignancies. The recent approval of inhibitors of poly (ADP-ribose) polymerase (iPARP) in the treatment of ovarian cancer in the presence of a BRCA1/2 mutation has sparked the analysis of women with such diagnosis, which can further benefit from the detection of carriers in the family. Germline sequence and large rearrangements for BRCA1/2 were tested in 398 consecutive epithelial ovarian cancer (EOC) patients.The aim of this study was to identify the frequency and spectrum of germline BRCA1/2 pathogenic alterations in a cohort of patients with ovarian serous carcinoma, with a view to adequately selecting patients for prevention through family counseling and correlating this frequency with platinum sensitivity as a guidance to identify patients eligible for iPARP in our population.Results
A total of 96 patients carried a pathogenic germline mutation, accounting for an overall 24.1% mutation incidence. Among mutation carriers, BRCA1 showed 62.5% incidence, BRCA2 rendered 36.5%, and one patient exhibited a mutation in both genes. Three pathogenic mutations were recurrent mutations detected five, three, and four times and represented 12.5% of the mutated samples. Worth highlighting, a 50% mutation incidence was detected when breast and ovarian cancer coexisted in the same patient. Novel mutations amounted to 9.4% of the total mutations, as compared to 4.7% in breast cancer. Forty out of 60 BRCA1 mutations were beyond the ovarian cancer cluster region (OCCR), in stark contrast with 22 out of 36 BRCA2 mutations being inside the OCCR. Taken together, germline BRCA1/2 mutations in EOC patients showed a distinct mutational spectrum compared to our previously published data on breast cancer patients.Conclusions
In sum, our study provides novel data on ovarian BRCA1/2 mutation prevalence worldwide, enhances adequate patient selection for family counseling and prevention, and sheds light on the benefits of iPARP treatment.110.
Liam Chung Erik Thiele Orberg Abby L. Geis June L. Chan Kai Fu Christina E. DeStefano Shields Christine M. Dejea Payam Fathi Jie Chen Benjamin B. Finard Ada J. Tam Florencia McAllister Hongni Fan Xinqun Wu Sudipto Ganguly Andriana Lebid Paul Metz Sara W. Van Meerbeke Franck Housseau 《Cell host & microbe》2018,23(2):203-214.e5