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51.
52.
An experimentally derived prediction tool is under development which aims to assess potential deactivation of diquat caused
by water and deposits on plant leaf surfaces in New Zealand water bodies, where aquatic weeds are targeted for diquat treatment.
Optimising the use and success of diquat is important not only in managing public confidence in the use of aquatic herbicides,
but also in minimising financial risk from failed treatments. Our approach focuses on characterising lake water quality and
plant condition factors in these lakes to identify parameters that might be useful indicators of diquat deactivation potential.
Water samples have been collected at 3-month intervals from lakes receiving large scale treatment for weed control. Samples
have been analysed for turbidity, suspended solids, chlorophyll a, conductivity and dissolved anions. Samples have also been spiked with 1 mg l−1 diquat to measure loss from adsorption and/or absorption. Shoot samples were also collected from targeted weed species at
each sampling site and the amount of organic and inorganic deposits on plants has been measured and then added to a second
diquat spiked sample to assess potential additional diquat loss from these deposits. Our results have shown deactivation from
deposits on plant surfaces which is highly correlated with turbidity, including inorganic suspended solids and total suspended
solids. A plant “dirtiness” scale has been devised to help predict the likely success or risk of diquat failure prior to any
decision to proceed with treatment. Deactivation in water was only weakly linked to total suspended solids. Our failure to
find significant correlation with the water quality factors measured may reflect the need for more detailed analysis of the
particle size and composition of suspended solids and future research will address these issues. 相似文献
53.
The tsetse fly vector transmits the protozoan Trypanosoma brucei, responsible for Human African Trypanosomiasis, one of the most neglected tropical diseases. Despite a recent decline in new cases, it is still crucial to develop alternative strategies to combat this disease. Here, we review the literature on the factors that influence trypanosome transmission from the fly vector to its vertebrate host (particularly humans). These factors include climate change effects to pathogen and vector development (in particular climate warming), as well as the distribution of host reservoirs. Finally, we present reports on the relationships between insect vector nutrition, immune function, microbiota and infection, to demonstrate how continuing research on the evolving ecology of these complex systems will help improve control strategies. In the future, such studies will be of increasing importance to understand how vector-borne diseases are spread in a changing world. 相似文献
54.
Champion de Crespigny FE Wedell N 《Proceedings. Biological sciences / The Royal Society》2006,273(1593):1455-1458
The maternally inherited bacterium Wolbachia pipientis imposes significant fitness costs on its hosts. One such cost is decreased sperm production resulting in reduced fertility of male Drosophila simulans infected with cytoplasmic incompatibility (CI) inducing Wolbachia. We tested the hypothesis that Wolbachia infection affects sperm competitive ability and found that Wolbachia infection is indeed associated with reduced success in sperm competition in non-virgin males. In the second male role, infected males sired 71% of the offspring whereas uninfected males sired 82% of offspring. This is the first empirical evidence indicating that Wolbachia infection deleteriously affects sperm competition and raises the possibility that polyandrous females can utilize differential sperm competitive ability to bias the paternity of broods and avoid the selfish manipulations of Wolbachia. This suggests a relationship between Wolbachia infection and host reproductive strategies. These findings also have important consequences for Wolbachia population dynamics because the transmission advantage of Wolbachia is likely to be undermined by sperm competition. 相似文献
55.
Fleur S. van Dijk Eline H. Zwikstra Sander R. Piersma Connie R. Jimenez Alice C. Morsman Mirjam H.H. van Roij Jonathan I.M.L. Verbeke Nick J. Shaw Carole McKeown Ann Dalton Gerard Pals 《American journal of human genetics》2009,85(4):521-527
Deficiency of cartilage-associated protein (CRTAP) or prolyl 3-hydroxylase 1(P3H1) has been reported in autosomal-recessive lethal or severe osteogenesis imperfecta (OI). CRTAP, P3H1, and cyclophilin B (CyPB) form an intracellular collagen-modifying complex that 3-hydroxylates proline at position 986 (P986) in the α1 chains of collagen type I. This 3-prolyl hydroxylation is decreased in patients with CRTAP and P3H1 deficiency. It was suspected that mutations in the PPIB gene encoding CyPB would also cause OI with decreased collagen 3-prolyl hydroxylation. To our knowledge we present the first two families with recessive OI caused by PPIB gene mutations. The clinical phenotype is compatible with OI Sillence type II-B/III as seen with COL1A1/2, CRTAP, and LEPRE1 mutations. The percentage of 3-hydroxylated P986 residues in patients with PPIB mutations is decreased in comparison to normal, but it is higher than in patients with CRTAP and LEPRE1 mutations. This result and the fact that CyPB is demonstrable independent of CRTAP and P3H1, along with reported decreased 3-prolyl hydroxylation due to deficiency of CRTAP lacking the catalytic hydroxylation domain and the known function of CyPB as a cis-trans isomerase, suggest that recessive OI is caused by a dysfunctional P3H1/CRTAP/CyPB complex rather than by the lack of 3-prolyl hydroxylation of a single proline residue in the α1 chains of collagen type I. 相似文献
56.
Kimberly M Korwek Justin H Trotter Mary Jo LaDu Patrick M Sullivan Edwin J Weeber 《Molecular neurodegeneration》2009,4(1):1-18
Accumulating evidence suggests that neurons prone to degeneration in Alzheimer's Disease (AD) exhibit evidence of re-entry into an aberrant mitotic cell cycle. Our laboratory recently demonstrated that, in a genomic amyloid precursor protein (APP) mouse model of AD (R1.40), neuronal cell cycle events (CCEs) occur in the absence of beta-amyloid (Aβ) deposition and are still dependent upon the amyloidogenic processing of the amyloid precursor protein (APP). These data suggested that soluble Aβ species might play a direct role in the induction of neuronal CCEs. Here, we show that exposure of non-transgenic primary cortical neurons to Aβ oligomers, but not monomers or fibrils, results in the retraction of neuronal processes, and induction of CCEs in a concentration dependent manner. Retraction of neuronal processes correlated with the induction of CCEs and the Aβ monomer or Aβ fibrils showed only minimal effects. In addition, we provide evidence that induction of neuronal CCEs are autonomous to primary neurons cultured from the R1.40 mice. Finally, our results also demonstrate that Aβ oligomer treated neurons exhibit elevated levels of activated Akt and mTOR (mammalian Target Of Rapamycin) and that PI3K, Akt or mTOR inhibitors blocked Aβ oligomer-induced neuronal CCEs. Taken together, these results demonstrate that Aβ oligomer-based induction of neuronal CCEs involve the PI3K-Akt-mTOR pathway. 相似文献
57.
Birgitte Tiesjema Susanne E. la Fleur Mieneke C.M. Luijendijk Roger A.H. Adan 《Obesity (Silver Spring, Md.)》2009,17(7):1448-1450
Increasing neuropeptide Y (NPY) signaling in the paraventricular nucleus (PVN) by recombinant adeno‐associated virus (rAAV)‐mediated overexpression of NPY in rats, results in hyperphagia and obesity in rats. To determine the importance of hyperphagia in the observed obesity phenotype, we pair‐fed a group of AAV‐NPY‐injected rats to AAV‐control‐injected rats and compared parameters of energy balance to ad libitum fed AAV‐NPY‐injected rats. For 3 weeks, AAV‐NPY‐injected rats, received the same amount of food as ad libitum‐fed rats injected with control rAAV They did not gain more body weight than these controls. When allowed access to food ad libitum, these AAV‐NPY‐injected rats increased food intake, which subsequently decreased when rats reached the same body weight as AAV‐NPY‐injected rats that were fed ad libitum for the entire study. These data indicate that overexpression of NPY in the PVN results in obesity by increasing food intake until a certain body weight is achieved. 相似文献
58.
Chun-Xia Yi Susanne E. la Fleur Eric Fliers Andries Kalsbeek 《生物化学与生物物理学报:疾病的分子基础》2010,1802(4):416-431
Despite a longstanding research interest ever since the early work by Claude Bernard, the functional significance of autonomic liver innervation, either sympathetic or parasympathetic, is still ill defined. This scarcity of information not only holds for the brain control of hepatic metabolism, but also for the metabolic sensing function of the liver and the way in which this metabolic information from the liver affects the brain. Clinical information from the bedside suggests that successful human liver transplantation (implying a complete autonomic liver denervation) causes no life threatening metabolic derangements, at least in the absence of severe metabolic challenges such as hypoglycemia. However, from the benchside, data are accumulating that interference with the neuronal brain–liver connection does cause pronounced changes in liver metabolism. This review provides an extensive overview on how metabolic information is sensed by the liver, and how this information is processed via neuronal pathways to the brain. With this information the brain controls liver metabolism and that of other organs and tissues. We will pay special attention to the hypothalamic pathways involved in these liver–brain–liver circuits. At this stage, we still do not know the final destination and processing of the metabolic information that is transferred from the liver to the brain. On the other hand, in recent years, there has been a considerable increase in the understanding which brain areas are involved in the control of liver metabolism via its autonomic innervation. However, in view of the ever rising prevalence of type 2 diabetes, this potentially highly relevant knowledge is still by far too limited. Thus the autonomic innervation of the liver and its role in the control of metabolism needs our continued and devoted attention. 相似文献
59.
Joshua Kurek Les C. Cwynar Russ C. Weeber Dean S. Jeffries John P. Smol 《Hydrobiologia》2010,652(1):207-221
Small, shallow lakes and ponds are often the dominant landscape features in many regions, but are comparably less studied
than larger lakes. Shallow lakes are more likely to lack fish populations; however, it is often difficult to ascertain whether
these sites were naturally fishless or lost their fish populations due to anthropogenic or natural stressors. We examined
the distributional abundances of four Chaoborus species by identifying and enumerating their larval mandibles in the surface sediments of 146 randomly selected lakes from
northwestern Ontario, Canada, to determine the key environmental gradients influencing chaoborid distributions. Chaoborus mandibles were encountered at 110 lakes and, in 65% of those lakes, total counts were ≥10. Direct gradient analyses were
then used to show that lakewater total aluminum concentrations (negatively correlated with pH), lakewater sodium concentrations,
lake surface area, and maximum water depth were significant predictors of the distributional abundances of Chaoborus. Generalized linear models indicated that Chaoborus species varied in their responses to significant environmental factors. C. (Sayomyia) was not significantly associated with any environmental variable and the abundances of larger chaoborids may be an important
biotic factor affecting this taxon. Chaoborus americanus, an indicator of fishless lakes, was significantly correlated with all five key variables and demonstrated a clear threshold
of occurrence in relatively small lakes (i.e., <10 ha in surface area). Furthermore, based on the occurrence and abundance
of C. americanus, we estimated that 20% of the lakes we surveyed are currently fishless. These lakes significantly differ in several geomorphic
and water-chemistry measures compared to the other study lakes. 相似文献
60.