全文获取类型
收费全文 | 1812篇 |
免费 | 184篇 |
国内免费 | 1篇 |
出版年
2021年 | 20篇 |
2019年 | 23篇 |
2018年 | 22篇 |
2017年 | 22篇 |
2016年 | 26篇 |
2015年 | 55篇 |
2014年 | 69篇 |
2013年 | 104篇 |
2012年 | 74篇 |
2011年 | 69篇 |
2010年 | 45篇 |
2009年 | 45篇 |
2008年 | 69篇 |
2007年 | 57篇 |
2006年 | 62篇 |
2005年 | 48篇 |
2004年 | 40篇 |
2003年 | 36篇 |
2002年 | 50篇 |
2001年 | 71篇 |
2000年 | 37篇 |
1999年 | 30篇 |
1998年 | 26篇 |
1997年 | 26篇 |
1996年 | 18篇 |
1995年 | 21篇 |
1994年 | 15篇 |
1993年 | 16篇 |
1992年 | 40篇 |
1991年 | 36篇 |
1990年 | 25篇 |
1989年 | 31篇 |
1988年 | 35篇 |
1987年 | 41篇 |
1986年 | 35篇 |
1985年 | 35篇 |
1984年 | 26篇 |
1983年 | 28篇 |
1982年 | 28篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1979年 | 14篇 |
1978年 | 28篇 |
1977年 | 21篇 |
1976年 | 23篇 |
1974年 | 29篇 |
1973年 | 24篇 |
1972年 | 17篇 |
1971年 | 21篇 |
1969年 | 16篇 |
排序方式: 共有1997条查询结果,搜索用时 15 毫秒
131.
Placental oxidative stress is a feature of human labor. Heat shock proteins (HSPs) play a key role in cellular stress. We hypothesized that placental expression of the small HSP 27 would be altered during labor and expression would vary in different regions of the placenta. Six women in labor who delivered vaginally and 6 women not in labor, who were delivered by Cesarean section, were recruited. Four equally spaced pieces were sampled from the inner, middle and outer regions of each placenta (total 12 samples per placenta). HSP 27 expression was investigated by Western blot analysis and RT-PCR. For non-labor, there was less HSP 27 protein in the inner placenta region compared with both the middle region (p<0.05) and outer region (p<0.05). For labor, there was also less HSP 27 protein in the inner region compared with both the middle (p<0.02) and outer region (p<0.01). When the 3 regions of the placenta were compared for non-labor versus labor there was less HSP 27 in the labor group at both the inner (p<0.05) and middle regions (p<0.005) compared to non-labor. Similar to HSP 27 protein, there was less HSP 27 mRNA in the labor group in both the inner region (p<0.05) and middle region (p<0.02) compared to non-labor. This study suggests that placental HSP 27 may play a role in labor and is spatially controlled. The results have important implications for how data obtained from studies in the placenta can be influenced by sampling methods. 相似文献
132.
133.
Corey Bragg Sam McKechnie Jamie Newman David Fletcher Henrik Moller Darren Scott 《New Zealand journal of zoology.》2013,40(3):275-289
Abstract Sooty shearwater (Puffinus griesus, titi) abundance, harvest levels and chick mass were monitored repeatedly on Putauhinu Island, south‐west of Rakiura (Stewart Island) between 1997 and 2005. Putauhinu is the second largest of the Titi Islands and has a relatively high density of chicks distributed over most of the island, so it supports what is likely the second‐largest population of sooty shearwaters in the Rakiura region (after Taukihepa, Big South Cape Island). Rakiura Maori harvested chicks from five “manu” (family birding areas) that covered 56% of the 128.4 ha of breeding colony of the island. Chick density was lower on the unharvested area in the interior of the island than on harvested areas. Burrow entrance density was higher where there was more ground cover (mainly fern) vegetation, but these areas had lower burrow occupancy, so overall chick density was similar at different levels of ground cover. Twenty‐six harvesters present on Putauhinu in 2005 took 31 280 chicks in total, equivalent to 8.4% (95% CI = 6.6–12%) of the available chicks on the entire island. Seasonal variation in total chicks harvested (CV 15–22%) was not related to chick abundance or mass. Refuges, including impenetrable patches of vegetated ground within manu, the unharvested centre of the island, and even nearby unharvested islands, will ameliorate localised impacts of harvest if density‐dependent immigration is operating. 相似文献
134.
135.
Shuiquan Tang Winnie W. M. Chan Kelly E. Fletcher Jana Seifert Xiaoming Liang Frank E. L?ffler Elizabeth A. Edwards Lorenz Adrian 《Applied and environmental microbiology》2013,79(3):974-981
Dehalococcoides mccartyi strains are obligate organohalide-respiring bacteria harboring multiple distinct reductive dehalogenase (RDase) genes within their genomes. A major challenge is to identify substrates for the enzymes encoded by these RDase genes. We demonstrate an approach that involves blue native polyacrylamide gel electrophoresis (BN-PAGE) followed by enzyme activity assays with gel slices and subsequent identification of proteins in gel slices using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). RDase expression was investigated in cultures of Dehalococcoides mccartyi strain BAV1 and in the KB-1 consortium growing on chlorinated ethenes and 1,2-dichloroethane. In cultures of strain BAV1, BvcA was the only RDase detected, revealing that this enzyme catalyzes the dechlorination not only of vinyl chloride, but also of all dichloroethene isomers and 1,2-dichloroethane. In cultures of consortium KB-1, five distinct Dehalococcoides RDases and one Geobacter RDase were expressed under the conditions tested. Three of the five RDases included orthologs to the previously identified chlorinated ethene-dechlorinating enzymes VcrA, BvcA, and TceA. This study revealed substrate promiscuity for these three enzymes and provides a path forward to further explore the largely unknown RDase protein family. 相似文献
136.
N. Fletcher I. E. Batjakas G. J. Pierce 《Zeitschrift fur angewandte Ichthyologie》2013,29(5):1030-1035
Seasonal and size‐related patterns in feeding habits of the Atlantic bonito (Sarda sarda) in the Northeast Aegean were investigated over a 2‐year period May 2007 – May 2009 based on analysis of stomach contents of 134 fish ranging from 266–655 mm fork length. The fish were caught by purse‐seine boats operating around the islands of Lesvos and Chios (Greece). Of the stomachs examined, 46% contained at least one prey item and significant seasonal and size‐related patterns were found in the occurrence of non‐empty stomachs. The overall diet composition in terms of numerical importance was 71% fish, 28% crustaceans and 1% cephalopods. Of the fish, Engraulidae (Engraulis encrasicolus) were the dominant prey taxon, followed by Centracanthidae (Spicara spp.) and then Clupeidae (Sardina pilchardus). Statistically significant monthly variation in diet was apparent, suggesting that S. sarda take advantage of seasonally abundant prey fish in the study area, but no size‐related variation in the importance of the main prey species was detected. 相似文献
137.
Capsule The incidence of sheep ticks on moorland wader chicks varied widely between species, however no effect on chick body condition was detected in relation to tick burden. 相似文献
138.
Burton Simon Jeffrey A. Fletcher Michael Doebeli 《Evolution; international journal of organic evolution》2013,67(6):1561-1572
The longstanding debate about the importance of group (multilevel) selection suffers from a lack of formal models that describe explicit selection events at multiple levels. Here, we describe a general class of models for two‐level evolutionary processes which include birth and death events at both levels. The models incorporate the state‐dependent rates at which these events occur. The models come in two closely related forms: (1) a continuous‐time Markov chain, and (2) a partial differential equation (PDE) derived from (1) by taking a limit. We argue that the mathematical structure of this PDE is the same for all models of two‐level population processes, regardless of the kinds of events featured in the model. The mathematical structure of the PDE allows for a simple and unambiguous way to distinguish between individual‐ and group‐level events in any two‐level population model. This distinction, in turn, suggests a new and intuitively appealing way to define group selection in terms of the effects of group‐level events. We illustrate our theory of group selection by applying it to models of the evolution of cooperation and the evolution of simple multicellular organisms, and then demonstrate that this kind of group selection is not mathematically equivalent to individual‐level (kin) selection. 相似文献
139.
Upland birds are predicted to be particularly vulnerable to the effects of climate change, yet few studies have examined these effects on their breeding phenology and productivity. Laying dates of Red Grouse Lagopus lagopus scotica in the Scottish Highlands advanced by 0.5 days/year between 1992 and 2011 and were inversely correlated with pre‐laying temperature, with a near‐significant increase in temperature over this period. Earlier clutches were larger and chick survival was greater in earlier nesting attempts. However, chick survival was also higher in years with lower May temperatures and lower August temperatures in the previous year, the latter probably related to prey abundance in the subsequent breeding season. Although laying dates are advancing, climate change does not currently appear to be having an overall effect on chick survival of Red Grouse within the climate range recorded in this study. 相似文献
140.
Jeremy L. Yap Huabo Wang Angela Hu Jay Chauhan Kwan-Young Jung Robert B. Gharavi Edward V. Prochownik Steven Fletcher 《Bioorganic & medicinal chemistry letters》2013,23(1):370-374
A structure–activity relationship (SAR) study of the c-Myc (Myc) inhibitor 10074-G5 (N-([1,1′-biphenyl]-2-yl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine, 1) – which targets a hydrophobic domain of the Myc oncoprotein that is flanked by arginine residues – was executed in order to determine its pharmacophore. Whilst the 7-nitrobenzofurazan was found to be critical for inhibitory activity, the ortho-biphenyl could be replaced with a para-carboxyphenyl group to furnish the new inhibitor JY-3-094 (3q). Around five times as potent as the lead with an IC50 of 33 μM for disruption of the Myc–Max heterodimer, JY-3-094 demonstrated excellent selectivity over Max–Max homodimers, with no apparent effect at 100 μM. Importantly, the carboxylic acid of JY-3-094 improves the physicochemical properties of the lead compound, which will facilitate the incorporation of additional hydrophobicity that might enhance Myc inhibitory activity further still. 相似文献