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991.
Fletcher L 《Nature biotechnology》2002,20(9):857-858
992.
Dong H Rothmel R Onstott TC Fuller ME DeFlaun MF Streger SH Dunlap R Fletcher M 《Applied and environmental microbiology》2002,68(5):2120-2132
The transport characteristics of two adhesion-deficient, indigenous groundwater strains, Comamonas sp. strain DA001 and Erwinia herbicola OYS2-A, were studied by using intact sediment cores (7 by 50 cm) from Oyster, Va. Both strains are gram-negative rods (1.10 by 0.56 and 1.56 by 0.46 microm, respectively) with strongly hydrophilic membranes and a slightly negative surface charge. The two strains exhibited markedly different behaviors when they were transported through granular porous sediment. To eliminate any effects of physical and chemical heterogeneity on bacterial transport and thus isolate the biological effect, the two strains were simultaneously injected into the same core. DA001 cells were metabolically labeled with (35)S and tagged with a vital fluorescent stain, while OYS2-A cells were metabolically labeled with (14)C. The fast decay of (35)S allowed deconvolution of the two isotopes (and therefore the two strains). Dramatic differences in the transport behaviors were observed. The breakthrough of DA001 and the breakthrough of OYS2-A both occurred before the breakthrough of a conservative tracer (termed differential advection), with effluent recoveries of 55 and 30%, respectively. The retained bacterial concentration of OYS2-A in the sediment was twofold higher than that of DA001. Among the cell properties analyzed, the statistically significant differences between the two strains were cell length and diameter. The shorter, larger-diameter DA001 cells displayed a higher effluent recovery than the longer, smaller-diameter OYS2-A cells. CXTFIT modeling results indicated that compared to the DA001 cells, the OYS2-A cells experienced lower pore velocity, higher porosity, a higher attachment rate, and a lower detachment rate. All these factors may contribute to the observed differences in transport. 相似文献
993.
994.
本文用非放射性法测定了K562,SP2/0,L-02,SPC-A-1,SMMC-7721和Hut-102等6个细胞培养液超离心沉淀物逆转录酶的含量,间接证明细胞培养物中逆转录病毒的存在。结果表明,Hut-102细胞为阳性,其它5个细胞系为阴性。 相似文献
995.
中国红豆杉属及其近缘植物的遗传变异和亲缘关系分析 总被引:11,自引:0,他引:11
采用水平式淀粉凝胶电泳技术,对中国产红豆杉属Taxus 3种1变种以及白豆杉Pseudotaxus
chienii共12个居群的遗传多样性进行了检测。对7个酶系统9个酶位点的分析结果表明,红豆杉属和
白豆杉的种内遗传多样性水平较高,各类群的多态位点比率(P)为44.4%~77.8%,等位基因平均数
(A)为1.6~2.1,平均期望杂合度(He)和实际观察杂合度(Ho)分别为0.065~0.152和0.068~0.111。
根据对等位酶实验结果的分析,结果表明除云南红豆杉T.yunnanensis与红豆杉属其它几个种的遗传
一致度稍低(I=0.727~0.804)外,红豆杉属的其它几个种的遗传一致度较高(0.906~0.995),体现其
亲缘关系很近。笔者建议将红豆杉属的这几个种合并为一个种,并根据地理分布及不同生境发生的变
异在种下设地理宗。同时根据等位酶的实验分析结果,白豆杉和红豆杉属各种的比较有显著差异,支持白豆杉作为单独属Pseudotaxus的处理。 相似文献
996.
Since pioneering work in the early 1960s, there has been growing interest and numerous experimental investigations into the
cryopreservation of algal material. Mostly, these studies relate to the requirement for long term preservation and storage
of algal material contained in culture collections or used in the seaweed mariculture industry. The present review deals with
techniques used in the cryopreservation of biological samples and their application to both micro- and macroalgae. Methods
for the prevention of cell damage and freezing injury during the cooling and low-temperature storage of algal material are
discussed with reference to the effect on viability of such variables as cooling rates, final temperatures attained, the use
of various types and concentrations of cryoprotectants, thawing rates, and storage times and temperatures. Some consideration
is also given to the various methods used for increasing cell viability, including the induction of freezing tolerance. Cryopreservation
protocols employed by numerous workers in this field are detailed, and concluding remarks are made on those techniques and
conditions providing optimum viability of cryopreserved algae.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
997.
998.
构建以CEA启动子控制HSV-TK基因表达的复制缺陷型腺病毒载体(AdCEATK).纯化的重组腺病毒滴度达1×1012pfu/ml.CEA阴性的HeLa细胞感染AdCMVTK后对丙氧鸟苷(GCV)很敏感,而感染了AdCEATK后不被GCV杀伤.与此相反CEA阳性的LoVo细胞中AdCMVTK和AdCEATK都有很好的表达活性,说明CEA启动子有良好的细胞专一性.AdCEATK/GCV系统还有明显的旁杀伤效应.此载体将有助于实现对CEA阳性肿瘤的专一性自杀基因治疗. 相似文献
999.
1000.
Paclobutrazol Protects Wheat Seedlings from Heat and Paraquat Injury. Is Detoxification of Active Oxygen Involved? 总被引:31,自引:0,他引:31
Paclobutrazol (PBZ), a member of the triazole family, protectedwheat (Triticum aestivum L.) seedlings from injury due to heat(50°C for 2.5 h) and paraquat (2 mM). Both stresses inducedphotoinhibition, loss of fresh weight and membrane integrity,suggesting the possible involvement of toxic oxygen species.This study tested the hypothesis that PBZ-induced protectionfrom stress is in part mediated by enhanced detoxification ofactive oxygen. The results support this hypothesis since PBZstimulated a 16, 32 and 21% increase in the activities of superoxidedismutase, ascorbate peroxidase (AP), and glutathione reductase(GR), on a fresh weight basis. The increased activities of APand GR were maintained to a higher degree than their correspondingcontrols after exposure to both heat and paraquat stress. Ascorbateand glutathione pools were l4 and 8% higher respectively inthe PBZ-treated wheat than in the controls. Within the cytoplasm,PBZ increased the activities of catalase (45%) and guaiacolperoxidase (29%) on a fresh weight basis and these higher activities,over the controls, were conserved after stress. It is suggestedthat the damage caused by two different stresses, heat and paraquat,is in part due to increased generation of active oxygen andthat PBZ protects plants by maintaining increased antioxidantenzyme activity. (Received May 10, 1993; Accepted October 29, 1993) 相似文献