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941.
FGF2 Antiproliferative Stimulation Induces Proteomic Dynamic Changes and High Expression of FOSB and JUNB in K‐Ras‐Driven Mouse Tumor Cells 下载免费PDF全文
Francisca Nathalia de Luna Vitorino Fabio Montoni Jaqueline Neves Moreno Bruno Ferreira de Souza Mariana de Camargo Lopes Barbara Cordeiro Cecilia Sella Fonseca Joshua M. Gilmore Mihaela I. Sardiu Marcelo Silva Reis Laurence A. Florens Michael P. Washburn Hugo Aguirre Armelin Julia Pinheiro Chagas da Cunha 《Proteomics》2018,18(17)
942.
Flavio E. Spetale Elizabeth Tapia Flavia Krsticevic Fernando Roda Pilar Bulacio 《PloS one》2016,11(1)
As volume of genomic data grows, computational methods become essential for providing a first glimpse onto gene annotations. Automated Gene Ontology (GO) annotation methods based on hierarchical ensemble classification techniques are particularly interesting when interpretability of annotation results is a main concern. In these methods, raw GO-term predictions computed by base binary classifiers are leveraged by checking the consistency of predefined GO relationships. Both formal leveraging strategies, with main focus on annotation precision, and heuristic alternatives, with main focus on scalability issues, have been described in literature. In this contribution, a factor graph approach to the hierarchical ensemble formulation of the automated GO annotation problem is presented. In this formal framework, a core factor graph is first built based on the GO structure and then enriched to take into account the noisy nature of GO-term predictions. Hence, starting from raw GO-term predictions, an iterative message passing algorithm between nodes of the factor graph is used to compute marginal probabilities of target GO-terms. Evaluations on Saccharomyces cerevisiae, Arabidopsis thaliana and Drosophila melanogaster protein sequences from the GO Molecular Function domain showed significant improvements over competing approaches, even when protein sequences were naively characterized by their physicochemical and secondary structure properties or when loose noisy annotation datasets were considered. Based on these promising results and using Arabidopsis thaliana annotation data, we extend our approach to the identification of most promising molecular function annotations for a set of proteins of unknown function in Solanum lycopersicum. 相似文献
943.
944.
Taiane de Azevedo Cardoso Thaise Campos Mondin Carolina David Wiener Melina Bazili Marques Briane de Ávila Fucolo Ricardo Tavares Pinheiro Luciano Dias Mattos de Souza Ricardo Azevedo da Silva Karen Jansen Jean Pierre Oses 《Neurochemical research》2014,39(8):1571-1578
Recent studies have evaluated the role of brain-derived neurotrophic factor (BDNF) in mood disorders; however, little is known about alterations in nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF). The aim of this study was to evaluate differences among serum neurotrophic factors (BDNF, NGF and GDNF) in depressed patients and healthy controls and to verify the association between serum neurotrophic levels and clinical characteristics in a young, depressed population stratified by gender. This is a cross-sectional study with depressed patients and population controls 18–29 years of age. The concentrations of neurotrophic factors were determined by the ELISA method. The diagnosis of depression and the duration of the disease were assessed by the Structured Clinical Interview according to the diagnostic and statistical manual of mental disorders. Depression severity was measured with the 17-item Hamilton Rating Scale for Depression, and the severity of anxiety symptoms was measured using the Hamilton Anxiety Rating Scale. Serum BDNF and GDNF were lower in major depressive disorder (MDD) patients compared to controls (p ≤ 0.001). Serum NGF levels were higher in MDD patients versus controls (p ≤ 0.001). BDNF was associated with the duration of disease only in women (p = 0.005). GDNF was not associated with clinical characteristics in either gender. In women, NGF was associated with the severity of depressive symptoms (p = 0.009), anxiety (p = 0.011) and disease duration (p = 0.005). NGF was associated with disease duration in men (p = 0.026). Our results demonstrated that significant neurochemical differences in NGF and BDNF, but not in GDNF, were associated with the clinical features of MDD when patients were stratified by gender. 相似文献
945.
Tibiriçá SH Bessa EC Mittherofhe A Castro MF Carvalho Odos S Caldeira RL Passos LK Mattos AM Pinheiro LS Sacramento e Silva D Bastos FO Andreoli GQ Bonato GR Coimbra ES 《Memórias do Instituto Oswaldo Cruz》2006,101(Z1):179-184
This study focuses on the geographic distribution of the snail of the genus Biomphalaria and evaluates its infectivity by Schistosoma mansoni in 5264 specimens collected in the municipality of Juiz de Fora, Minas Gerais, Brazil. Of the 31 locations studied, 6 were reservoirs, 11 rudimentary holding ponds, 7 irrigation ditches, 5 lakes, 1 ornamental pond, and 1 waterfall. Intermediate hosts were found only in the rudimentary ponds and ditches, which were 100% positive. Using morphological and molecular analysis techniques, B. tenagophila, B. peregrina, and B. straminea were identified. This is the first report of B. stramínea in the municipality, and evaluation of its infective potential revealed susceptibility of 25.4%. Although we did not find specimens of Biomphalaria infected by S. mansoni, the data obtained indicate the presence of intermediate hosts, especially in the irrigation ditches in Juiz de Fora, and their proximity to contaminated areas. 相似文献
946.
Evelyn Jardim Oliveira Andréa Dias Koehler Diego Ismael Rocha Lorena Melo Vieira Marcos Vinícius Marques Pinheiro Elyabe Monteiro de Matos Ana Claudia Ferreira da Cruz Thais Cristina Ribeiro da Silva Francisco André Ossamu Tanaka Fabio Tebaldi Silveira Nogueira Wagner Campos Otoni 《Protoplasma》2017,254(5):2017-2034
947.
In vivo conversion of [4-14C]sitosterol and [22,23-3H]sitosterol to stigmasterol in barley seedlings
Six-day-old barley seedlings were allowed to take up [4-14C]sitosterol and [22, 23-3H]sitosterol for 2.5 hr and the incorporation into the sterol fractions was determined after 0, 6, 12 and 24 hr. Sitosterol was readily incorporated into every sterol class. The 3H/14C ratio in the free forms dropped when compared with the 3H/14C ratio of the administered sitosterol. In the free sterol, radioactive stigmasterol, showing a 3H/14C ratio half that of the sitosterol 3H/14C ratio, was isolated and its radiochemical purity established by dilution with carrier material and crystallization to constant specific activity. 相似文献
948.
949.
Alan Fecchio Jeffrey A. Bell Rafael B.P. Pinheiro Victor R. Cueto Cristian A. Gorosito Holly L. Lutz Milene G. Gaiotti Luciana V. Paiva Leonardo F. Frana Guilherme Toledo‐Lima Mariana Tolentino Joo B. Pinho Vasyl V. Tkach Carla S. Fontana Juan Manuel Grande Miguel A. Santilln Renato Caparroz Andrei L. Roos Rafael Bessa Wagner Nogueira Thiago Moura Erica C. Nolasco Kiba J.M. Comiche Karin Kirchgatter Lilian O. Guimares Janice H. Dispoto Miguel . Marini Jason D. Weckstein Henrique Batalha‐Filho Michael D. Collins 《Molecular ecology》2019,28(10):2681-2693
Identifying the ecological factors that shape parasite distributions remains a central goal in disease ecology. These factors include dispersal capability, environmental filters and geographic distance. Using 520 haemosporidian parasite genetic lineages recovered from 7,534 birds sampled across tropical and temperate South America, we tested (a) the latitudinal diversity gradient hypothesis and (b) the distance–decay relationship (decreasing proportion of shared species between communities with increasing geographic distance) for this host–parasite system. We then inferred the biogeographic processes influencing the diversity and distributions of this cosmopolitan group of parasites across South America. We found support for a latitudinal gradient in diversity for avian haemosporidian parasites, potentially mediated through higher avian host diversity towards the equator. Parasite similarity was correlated with climate similarity, geographic distance and host composition. Local diversification in Amazonian lineages followed by dispersal was the most frequent biogeographic events reconstructed for haemosporidian parasites. Combining macroecological patterns and biogeographic processes, our study reveals that haemosporidian parasites are capable of circumventing geographic barriers and dispersing across biomes, although constrained by environmental filtering. The contemporary diversity and distributions of haemosporidian parasites are mainly driven by historical (speciation) and ecological (dispersal) processes, whereas the parasite community assembly is largely governed by host composition and to a lesser extent by environmental conditions. 相似文献
950.
Single exposure of human fibroblasts (WI-38) to a sub-cytotoxic dose of UVB induces premature senescence 总被引:1,自引:0,他引:1
Straface E Vona R Ascione B Matarrese P Strudthoff T Franconi F Malorni W 《FEBS letters》2007,581(22):4342-4348
In this work, we present a new model of stress-induced premature senescence obtained by exposing human fibroblasts (WI-38) at early passages (passages 2-4) to a single sub-cytotoxic dose of UVB (200 mJ/cm(2)). We show that this treatment leads to the appearance of several biomarkers of senescence such as enlarged and flattened cell morphology, the presence of nuclear heterochromatic foci and beta-galactosidase activity. Furthermore, we demonstrate that a mild ROS production and p53 activation are upstream events required for the induction of premature senescence. Our method represents an alternative in vitro model in photoaging research and could be used to test potential anti-photoaging compounds. 相似文献