全文获取类型
收费全文 | 195篇 |
免费 | 17篇 |
出版年
2022年 | 2篇 |
2021年 | 5篇 |
2019年 | 2篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 9篇 |
2014年 | 8篇 |
2013年 | 10篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 9篇 |
2009年 | 8篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 10篇 |
2005年 | 9篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 7篇 |
1999年 | 8篇 |
1998年 | 8篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1970年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1956年 | 1篇 |
1953年 | 1篇 |
1946年 | 1篇 |
1943年 | 1篇 |
1940年 | 1篇 |
1939年 | 1篇 |
1936年 | 1篇 |
排序方式: 共有212条查询结果,搜索用时 15 毫秒
191.
Sarfraz A Tunio Neil J Oldfield Dlawer AA Ala'Aldeen Karl G Wooldridge David PJ Turner 《BMC microbiology》2010,10(1):280
Background
Glyceraldehyde 3-phosphate dehydrogenases (GAPDHs) are cytoplasmic glycolytic enzymes, which although lacking identifiable secretion signals, have also been found localized to the surface of several bacteria (and some eukaryotic organisms); where in some cases they have been shown to contribute to the colonization and invasion of host tissues. Neisseria meningitidis is an obligate human nasopharyngeal commensal which can cause life-threatening infections including septicaemia and meningitis. N. meningitidis has two genes, gapA-1 and gapA-2, encoding GAPDH enzymes. GapA-1 has previously been shown to be up-regulated on bacterial contact with host epithelial cells and is accessible to antibodies on the surface of capsule-permeabilized meningococcal cells. The aims of this study were: 1) to determine whether GapA-1 was expressed across different strains of N. meningitidis; 2) to determine whether GapA-1 surface accessibility to antibodies was dependant on the presence of capsule; 3) to determine whether GapA-1 can influence the interaction of meningococci and host cells, particularly in the key stages of adhesion and invasion. 相似文献192.
193.
194.
Plasma membrane injury is a frequent event, and wounds have to be rapidly repaired to ensure cellular survival. Influx of Ca2+ is a key signaling event that triggers the repair of mechanical wounds on the plasma membrane within ~30 sec. Recent studies revealed that mammalian cells also reseal their plasma membrane after permeabilization with pore forming toxins in a Ca2+-dependent process that involves exocytosis of the lysosomal enzyme acid sphingomyelinase followed by pore endocytosis. Here, we describe the methodology used to demonstrate that the resealing of cells permeabilized by the toxin streptolysin O is also rapid and dependent on Ca2+ influx. The assay design allows synchronization of the injury event and a precise kinetic measurement of the ability of cells to restore plasma membrane integrity by imaging and quantifying the extent by which the liphophilic dye FM1-43 reaches intracellular membranes. This live assay also allows a sensitive assessment of the ability of exogenously added soluble factors such as sphingomyelinase to inhibit FM1-43 influx, reflecting the ability of cells to repair their plasma membrane. This assay allowed us to show for the first time that sphingomyelinase acts downstream of Ca2+-dependent exocytosis, since extracellular addition of the enzyme promotes resealing of cells permeabilized in the absence of Ca2+. 相似文献
195.
196.
Alessandro Pecego Martins Romano Zouraide Guerra Antunes Costa Daniel Garkauskas Ramos Maria Auxiliadora Andrade Valéria de Sá Jayme Marco Ant?nio Barreto de Almeida Kátia Campomar Vettorello Melissa Mascheretti Brendan Flannery 《PLoS neglected tropical diseases》2014,8(3)
Due to the risk of severe vaccine-associated adverse events, yellow fever vaccination in Brazil is only recommended in areas considered at risk for disease. From September 2008 through June 2009, two outbreaks of yellow fever in previously unvaccinated populations resulted in 21 confirmed cases with 9 deaths (case-fatality, 43%) in the southern state of Rio Grande do Sul and 28 cases with 11 deaths (39%) in Sao Paulo state. Epizootic deaths of non-human primates were reported before and during the outbreak. Over 5.5 million doses of yellow fever vaccine were administered in the two most affected states. Vaccine-associated adverse events were associated with six deaths due to acute viscerotropic disease (0.8 deaths per million doses administered) and 45 cases of acute neurotropic disease (5.6 per million doses administered). Yellow fever vaccine recommendations were revised to include areas in Brazil previously not considered at risk for yellow fever. 相似文献
197.
Rebecca L. Renberg Xiaojing Yuan Tamika K. Samuel Danilo C. Miguel Iqbal Hamza Norma W. Andrews Andrew R. Flannery 《PLoS neglected tropical diseases》2015,9(5)
Leishmania spp. are trypanosomatid parasites that replicate intracellularly in macrophages, causing serious human morbidity and mortality throughout the world. Trypanosomatid protozoa cannot synthesize heme, so must acquire this essential cofactor from their environment. Earlier studies identified LHR1 as a Leishmania amazonensis transmembrane protein that mediates heme uptake. Null mutants of LHR1 are not viable and single knockout strains have reduced virulence, but very little is known about the properties of LHR1 directly associated with heme transport. Here, we use functional assays in Saccharomyces cerevisiae to show that specific tyrosine residues within the first three predicted transmembrane domains of LHR1 are required for efficient heme uptake. These tyrosines are unique to LHR1, consistent with the low similarity between LHR1 and its corresponding homologs in C. elegans and human. Substitution of these tyrosines in LHR1 resulted in varying degrees of heme transport inhibition, phenotypes that closely mirrored the impaired ability of L. amazonensis to replicate as intracellular amastigotes in macrophages and generate cutaneous lesions in mice. Taken together, our results imply that the mechanism for heme transport by LHR1 is distinctive and may have adapted to secure heme, a limiting cofactor, inside the host. Since LHR1 is significantly divergent from the human heme transporter HRG1, our findings lay the groundwork for selective targeting of LHR1 by small molecule antagonists. 相似文献
198.
Mechanisms involved in cartilage proteoglycan catabolism. 总被引:19,自引:0,他引:19
The increased catabolism of the cartilage proteoglycan aggrecan is a principal pathological process which leads to the degeneration of articular cartilage in arthritic joint diseases. The consequent loss of sulphated glycosaminoglycans, which are intrinsic components of the aggrecan molecule, compromises both the functional and structural integrity of the cartilage matrix and ultimately renders the tissue incapable of resisting the compressive loads applied during joint articulation. Over time, this process leads to irreversible cartilage erosion. In situ degradation of aggrecan is a proteolytic process involving cleavage at specific peptide bonds located within the core protein. The most well characterised enzymatic activities contributing to this process are engendered by zinc-dependent metalloproteinases. In vitro aggrecanolysis by matrix metalloproteinases (MMPs) has been widely studied; however, it is now well recognised that the principal proteinases responsible for aggrecan degradation in situ in articular cartilage are the aggrecanases, two recently identified isoforms of which are members of the 'A Disintegrin And Metalloproteinase with Thrombospondin motifs' (ADAMTS) gene family. In this review we have described: (i) the development of monoclonal antibody technologies to identify catabolic neoepitopes on aggrecan degradation products; (ii) the use of such neoepitope antibodies in studies designed to characterise and identify the enzymes responsible for cartilage aggrecan metabolism; (iii) the biochemical properties of soluble cartilage aggrecanase(s) and their differential expression in situ; and (iv) model culture systems for studying cartilage aggrecan catabolism. These studies have clearly established that 'aggrecanase(s)' is primarily responsible for the catabolism and loss of aggrecan from articular cartilage in the early stages of arthritic joint diseases that precede overt collagen catabolism and disruption of the tissue integrity. At later stages, when collagen catabolism is occurring, there is evidence for MMP-mediated degradation of the small proportion of aggrecan remaining in the tissue, but this occurs independently of continued aggrecanase activity. Furthermore, the catabolism of link proteins by MMPs is also initiated when overt collagen degradation is evident. 相似文献
199.
Patricia Cohen Barbara Connetta Douglas Dix John Flannery 《Journal of theoretical biology》1981,90(3):427-436
Most hematologic tumors (acute and chronic myelogenous leukemia, chronic lymphatic leukemia, polycythemia vera, and multiple myeloma) exhibit an exponential increase in incidence with advancing age of the host. This age-incidence pattern resembles that for carcinomas and can be explained by the accumulation of harmful mutations in the stem cells of the tissues of tumor origin during the course of normal aging. The age-incidence pattern for acute lymphatic leukemia is unique and complex with a linear increase in incidence from birth to age 3, an exponential decline in incidence form age 3 to 34, a low and constant rate of incidence from age 34 to 60, and a slight increase in incidence at ages greater than 60. We conclude that variation in tumor incidence with host age is determined by the pattern of cell proliferation in the tissue of tumor origin. We suggest that cell proliferation in the tissue of origin of acute lymphatic leukemia occurs in stages: (1) rapid stem cell proliferation from before birth to age 3, (2) random stem cell differentiation from age 3 to 34, and (3) a constant rate of stem cell proliferation and differentiation after age 34. 相似文献
200.