The application of the probit method to toxicity test data adjusted for mortality in the controls

When data from toxicity tests have to be adjusted for a mortality rate amongst untreated controls, three modifications to the usual method of probit analysis need to be considered. The full solution is more laborious than that ordinarily used, as it requires the estimation of this mortality rate from the whole of the data. When the rate is. not high (e.g. up to 20 %) a satisfactory approximation may be obtained by a simple alteration in die weighting coefficients, especially if the precaution is taken of using two or three times as many test organisms as controls as for any dosage of the poison, thus decreasing the relative value of the information on natural mortality contributed by the treated batches. The modified weighting coefficients are tabulated for values of the natural mortality from zero to 20 %. An example of the more complex calculations required for the full maximum likelihood solution is discussed in detail.     

Symbols and Terminology in Biometry

The many biologists whose work requires statistical science must be concerned for sound management and interpretation of quantitative data. Biometricians also need increased care for literacy and clarity in speech and writing, with precise phrasing for every numerical statement. This paper illustrates common confusions that arise from inexact terminology, or from words and symbols used without adequate definition. Careless statements on quantitative relations, or on probabilities, may be ambiguous; bad practices seriously pollute scientific journals and obstruct transmission of information. Such faults can affect daily life for a modern citizen. Pedantry is unwanted, and to be dogmatic about corrective measures would be stupid. This paper suggests that biologists and biometricians should examine the practicability of a system yet to be devised for standardizing use of symbols and the generally accepted terminology for the methods, techniques, and processes of statistical analysis. The outcome should influence all that we biometricians say and do — as authors, as consultants, and as referees for journals.     

根施甜菜碱对镍胁迫下小麦幼苗生长生理的影响

以普通小麦品种‘轮选988’为材料,采用溶液培养法,研究了根施不同浓度甜菜碱(1.0、2.0、3.0、4.0、5.0、10.0、15.0、20.0mmol·L~(-1))对镍(100μmol·L~(-1) NiSO_4)胁迫下小麦根系生长的影响,以及4.0mmol·L~(-1)甜菜碱处理镍胁迫幼苗根系相关抗逆生理生化指标的变化。结果表明:(1)与不施加镍对照相比,镍胁迫下小麦幼苗的根长、株高、鲜重和干重分别显著降低了14.7%、11.7%、15.0%和16.7%。(2)与单独镍胁迫处理相比,小麦幼苗的根长、株高、鲜重和干重均随着根施甜菜碱的浓度逐渐增加且呈先升后降的趋势,并以4.0mmol·L~(-1)外源甜菜碱处理效果较佳。(3)与单独镍胁迫处理相比较,在4.0mmol·L~(-1)外源甜菜碱处理下,小麦幼苗根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性分别升高了284.7%、40.3%、82.9%和20.4%,超氧阴离子自由基(O-·2)含量、过氧化氢(H_2O_2)含量和丙二醛(MDA)含量分别显著降低了50.6%、38.4%和40.6%,可溶性糖含量及游离脯氨酸(Pro)含量分别显著降低了19.2%、45.4%,而根系活力大幅上升了358.0%。研究认为,根施适宜浓度外源甜菜碱可显著增强小麦幼苗根系的抗氧化能力,恢复根系活力,从而有效减弱镍胁迫对小麦幼苗生长的伤害。     

Surface N balances and reactive N loss to the environment from global intensive agricultural production systems for the period 1970-2030

Data for the historical years 1970 and 1995 and the FAO-Agriculture Towards 2030 projection are used to calculate N inputs (N fertilizer, animal manure, biological N fixation and atmospheric deposition) and the N export from the field in harvested crops and grass and grass consumption by grazing animals. In most industrialized countries we see a gradual increase of the overall N recovery of the intensive agricultural production systems over the whole 1970-2030 period. In contrast, low N input systems in many developing countries sustained low crop yields for many years but at the cost of soil fertility by depleting soil nutrient pools. In most developing countries the N recovery will increase in the coming decades by increasing efficiencies of N use in both crop and livestock production systems. The surface balance surplus of N is lost from the agricultural system via different pathways, including NH3 volatilization, denitrification, N2O and NO emissions, and nitrate leaching from the root zone. Global NH3-N emissions from fertilizer and animal manure application and stored manure increased from 18 to 34 Tg·yr-1 between 1970 and 1995, and will further increase to 44 Tg·yr-1 in 2030. Similar developments are seen for N2O-N (2.0 Tg·yr-1 in 1970, 2.7 Tg·yr-1 in 1995 and 3.5 Tg·yr-1 in 2030) and NO-N emissions (1.1 Tg·yr-1 in 1970, 1.5Tg·yr-1 in 1995 and 2.0 Tg·yr-1 in 2030).     

Paleoceanography of the Gulf of Alaska during the past 15,000 years: Results from diatoms, silicoflagellates, and geochemistry

High-resolution records of diatoms, silicoflagellates, and geochemistry covering the past 15,000 years were studied in three cores from the Gulf of Alaska (GOA). Core EW0408-85JC in an oceanic setting on the Kayak Slope displays a paleoceanographic record similar to that at several locations on the California margin during deglaciation. Biologic productivity as reconstructed using geochemical and microfossil proxies increased abruptly during the Bølling–Alleröd (Bø–Al) warm interval (14.7–12.9 cal ka), declined during the Younger Dryas (YD) cold interval (12.9 to 11.7 cal kyr BP), and rose again during the earliest Holocene. At this site, the record after ~ 11 cal kyr BP is dominated by oceanic diatoms and silicoflagellates, with geochemical proxies displaying more subtle variation.Cores EW0408-66JC in the Yakobi Sea Valley near Cross Sound and EW0408-11JC in the Gulf of Esquibel contain an expanded, composite record along the southeast Alaskan margin. Core 66JC contains a detailed record of the Bø–Al and YD. Diatoms and silicoflagellates indicate that coastal upwelling and biosiliceous productivity were strong during the Bø–Al but declined during the YD. Sea ice-related diatoms increased in abundance during the YD, indicating cooler, but less productive waters.The glacial to biogenic marine sediment transition in core 11JC occurs at 1280 cmbsf (centimeters below sea floor), probably representing rising sea level and deglaciation early in the Bø–Al. Freshwater and sea-ice related diatoms are common in the lower part of the core (Bø–Al and YD), but upwelling-related diatoms and silicoflagellates quickly increased in relative abundance up-core, dominating the record of the past 11,000 years. Low oxygen conditions in the bottom water as reconstructed using geochemical proxies (U and Mo concentration) were most intense between ~ 6.5 and 2.8 cal kyr BP, the beginning of which is coincident with increases in abundance of upwelling-related diatoms.The records from these three cores jointly thus made it possible to reconstruct paleoclimatic and paleoceanographic conditions at high northern Pacific latitudes during the last 15 kyr.     

Artificial T-cell receptors

Artificial T-cell receptors are generated by joining an Ag-recognizing domain (ectodomain) to the transmembrane and intracellular portion of a signaling molecule (endodomain). The ectodomain is most often derived from Ab variable chains, but may also be generated from T-cell receptor variable chains, as well as from other molecules. Various alternative ectodomain designs exist, with some comparative studies suggesting optimal forms. The endodomain most often used is the intracellular portion of CD-zeta. Although signaling by CD-zeta leads to IFN-n release and cell killing, it fails to transmit a full activation signal. Recently, unions of different signaling molecule segments have facilitated transmission of more potent signals, stimulating T-cell proliferation and overcoming this major limitation. Artificial T-cell receptors allow grafting of nearly any specificity to T cells. This allows generation of large numbers of specific T cells, without laborious selection and expansion procedures. Efficacy against tumors has been demonstrated in animal models. Phase I and II studies of T-cells transduced with artificial T-cell receptors as therapy for HIV infection have been performed. This rapidly advancing technology will make new strategies of adoptive immunotherapy possible.     

Isocitrate dehydrogenase 1 R132C mutation occurs exclusively in microsatellite stable colorectal cancers with the CpG island methylator phenotype

The CpG Island Methylator Phenotype (CIMP) is fundamental to an important subset of colorectal cancer; however, its cause is unknown. CIMP is associated with microsatellite instability but is also found in BRAF mutant microsatellite stable cancers that are associated with poor prognosis. The isocitrate dehydrogenase 1 (IDH1) gene causes CIMP in glioma due to an activating mutation that produces the 2-hydroxyglutarate oncometabolite. We therefore examined IDH1 alteration as a potential cause of CIMP in colorectal cancer. The IDH1 mutational hotspot was screened in 86 CIMP-positive and 80 CIMP-negative cancers. The entire coding sequence was examined in 81 CIMP-positive colorectal cancers. Forty-seven cancers varying by CIMP-status and IDH1 mutation status were examined using Illumina 450K DNA methylation microarrays. The R132C IDH1 mutation was detected in 4/166 cancers. All IDH1 mutations were in CIMP cancers that were BRAF mutant and microsatellite stable (4/45, 8.9%). Unsupervised hierarchical cluster analysis identified an IDH1 mutation-like methylation signature in approximately half of the CIMP-positive cancers. IDH1 mutation appears to cause CIMP in a small proportion of BRAF mutant, microsatellite stable colorectal cancers. This study provides a precedent that a single gene mutation may cause CIMP in colorectal cancer, and that this will be associated with a specific epigenetic signature and clinicopathological features.     

双源CT冠状动脉血管成像诊断心肌桥的价值探讨

目的：研究双源CT冠状动脉血管成像诊断心肌桥的临床价值。方法：选择260例具有典型心前区不适的患者进行双源CT冠脉血管成像检查,观察其发生部位,测量其长度和深度并进行分析。结果：260例受检患者中,62例共70段存在心肌桥,检出率达20.76%,高于文献报道的检出率18.2%。所有心肌桥均发生于左前降支,其中近段17段（24.4%）,中段43段（61.4%）,远段10段（14.2%）。心肌桥平均长度为15.8±6.4mm,深度为1.4±0.85mm。结论：双源CT冠状动脉血管成像因其便捷无创,不受心率严格限制且价格低廉可作为心肌桥筛查的理想检查手段。     

羊草与其主要伴生种竞争与共存的格局分析

在羊草种群与其它植物种群的交错区,应用频度、格避形式,格局强度指数对羊草及其主要伴生种之间的共存格局进行了分析。结果表明,羊草及其主要伴生种的格局呈多样化,集聚格局形式是羊草抵御外来物种入侵,或者是自身扩散的一种对策,羊草与其主要伴生种之间存在竞争与共存作用,羊草与芦苇之间通过拮抗作用实现竞争与共存,羊草与鸡儿肠通过竞争而实现共存,光稃茅香,碱茅以营养繁殖策略实现与羊草竞争,指子茅的生长受羊草竞争的抑制。