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801.
802.
Psychrophilic microorganisms and their cold-active enzymes 总被引:2,自引:0,他引:2
JE Brenchley 《Journal of industrial microbiology & biotechnology》1996,17(5-6):432-437
803.
I. Rosenshie B. Brett Finlay 《BioEssays : news and reviews in molecular, cellular and developmental biology》1993,15(1):17-24
Many bacteria that cause disease have the capacity to enter into and live within eukaryotic cells such as epithelial cells and macrophages. The mechanisms used by these organisms to achieve and maintain this intracellular lifestyle vary considerably, but most mechanisms involve subversion and exploitation of host cell functions. Entry into non-phagocytic cells involves triggering host signal transduction mechanisms to induce rearrangement of the host cytoskeleton, thereby facilitating bacterial uptake. Once inside the host cell, intracellular pathogens either remain within membrane bound inclusions or escape to the cytoplasm. Those living in the cytoplasm can further pirate the host actin system, using actin as a mechanism to facilitate movement within and between host cells. Organisms remaining within the vacuole have specialized mechanisms for intracellular survival and growth which involve additional communication with the host cell. Some of the processes involved in the various steps of facultative intracellular parasitism are discussed in the context of subverting the host cell cytoskeleton and signal transduction pathways for bacterial benefit. 相似文献
804.
Chronic exposure to hydrocortisone (HC) or dexamethasone (DEX) results in a 20-40% extension in the proliferative lifespan of WI38 cells. Within a single growth cycle, the addition of HC or DEX at seeding results in saturation densities 20-40% higher than in control cultures. We have recently reported that, within a single growth cycle, the proliferative response of WI38 cells to glucocorticoids is mediated by a stimulatory factor(s) present in medium conditioned by cells in the presence of the hormone. We report here that chronic exposure to medium conditioned in the presence of HC for the first 24 h after seeding (24-h HC-conditioned medium (24-h HC-CM)) results in a 25% extension in the proliferative lifespan of these cultures. The generation of the stimulatory factor(s) present in glucocorticoid-conditioned medium is apparently dependent upon undefined cellular alterations which result from the subcultivation-procedure; confluent or low-density quiescent cultures did not generate media stimulatory to cell growth in the presence of glucocorticoids. This response was not trypsin-dependent, since cultures subcultivated in the absence of proteolytic treatment generated media equally stimulatory to cell growth. A further characterization of this glucocorticoid-induced activity revealed the stimulatory factor(s) was of low MW (dialyzable and recoverable in the less than 10,000 MW fraction following ultrafiltration), heat-stable (95 degrees C), and resistant to treatment with trypsin, chymotrypsin, or protease (S. griseus). 相似文献
805.
Cyclins are indispensable elements of the cell cycle and derangement of their function can lead to cancer formation. Recent studies have also revealed more mechanisms through which cyclins can express their oncogenic potential. This review focuses on the aberrant expression of G1/S cyclins and especially cyclin D and cyclin E; the pathways through which they lead to tumour formation and their involvement in different types of cancer. These elements indicate the mechanisms that could act as targets for cancer therapy. 相似文献
806.
807.
Pål Axel Olsson Michel Chalot Erland Bååth Roger D. Finlay Bengt Söderström 《FEMS microbiology ecology》1996,21(2):77-86
Abstract: Bacterial activity was studied in a growth system containing Pinus contorta seedlings inoculated with different mycorrhizal fungi. Nylon nets enabled separation of soil compartments with extramatrical mycorrhizal hyphae from soil compartments with roots and mycelium. In three separate experiments bacterial activity, estimated as thymidine incorporation, was reduced in soils with Paxillus involutus hyphae compared to controls without mycorrhizal hyphae. This effect was found irrespective of compartments with and without roots were compared. Laccaria bicolor only reduced the activity in one of these three experiments. Thelephora terrestris (tested in two experiments), Laccaria proxima, Suillus variegatus and Hebeloma crustuliniforme (one experiment), also reduced the thymidine and leucine incorporation rates of bacteria. The reduction for these fungi varied between 20% and 50% in all experiments. Numbers of viable bacteria appeared to be reduced by T. terrestris, L. proxima, S. variegatus and H. crustuliniforme in one experiment, while no effect was seen in the other experiments. 相似文献
808.
S Capewell S Reynolds D Shuttleworth C Edwards A Y Finlay 《BMJ (Clinical research ed.)》1990,300(6739):1548-1551
OBJECTIVE--To assess the effect of high dose inhaled corticosteroids on skin. DESIGN--Cross sectional study of patients receiving treatment for chest diseases. SETTING--Outpatient chest clinic in a teaching hospital. PATIENTS--68 Patients divided into four groups of similar age--namely, 15 receiving long term oral prednisolone, 21 receiving high dose inhaled corticosteroids, 15 receiving low dose inhaled corticosteroids, and 17 controls. MAIN OUTCOME MEASURES--Skin thickness at three sites measured by A scan ultrasound and clinical assessment of purpura. RESULTS--Compared with controls patients in both the oral prednisolone treated group and the high dose inhaled corticosteroid treated group had significantly thinner skin at all three sites (group median thicknesses: prednisolone treated group 28-33% less than controls; high dose inhaled corticosteroid treated group 15-19% less than controls). Differences in skin thicknesses between the low dose inhaled corticosteroid treated group and the controls were trivial. The prevalence of purpura was significantly greater in patients receiving oral prednisolone (12/15 patients) and high dose inhaled corticosteroids (10/21) than in controls (2/17). CONCLUSION--Skin thinning and purpura represent further evidence of systemic effects of high dose inhaled corticosteroids. 相似文献
809.
Cell adhesion and invasion mechanisms in microbial pathogenesis 总被引:10,自引:0,他引:10
B B Finlay 《Current opinion in cell biology》1990,2(5):815-820