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61.
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63.
The relationship between chromosomal nondisjunction and semen quality was studied in two groups of males who differ highly
in their semen quality: 12 individuals with low-quality semen caused by varicocele, and 8 subjects with high-quality semen,
selected from sperm donors for in vitro fertilization. Chromosomal nondisjunction was inferred from the rate of disomy found
in mature sperm cells. To determine the rate of disomy, we applied fluorescence in situ hybridization using satellite-specific
probes for chromosomes 1, 15, 18, X and Y. In sperm cells of males with low-quality semen, the mean rate of disomy for each
of the autosomes and of hetero-disomy for the sex chromosomes (XY) was significantly higher than that observed in the high-quality
semen samples: more than 15-fold higher for chromosomes 1 and 15, and 7-fold higher for chromosomes 18 and XY. Yet, the homo-disomy
rate for each of the sex chromosomes (XX and YY) was almost the same in both types of semen. The large discrepancy between
the low- and high-quality semen in the rate of sex chromosome hetero-disomy versus the similar rate of homo-disomy strongly
suggests that the abnormal chromosomal segregation in meiocytes of males with low-quality semen resulted from chromosomal
nondisjunction at the first meiotic division. The results indicate that men showing poor semen quality are at an increased
risk for meiotic nondisjunction, similar to women at the end of their reproductive years.
Received: 30 June 1997 / Accepted: 17 September 1997 相似文献
64.
The study of anisotropy light scattering from tapioca and potato starches has continued with the recording of more detailed experimental single-granule Hv scattering patterns and, for the first time, single-granule Vv patterns. Quantitative analysis of the higher order scattering maxima to the granule morphology, permitting an analysis of the latter in terms of a lyered structure. For tapioca starch, this analysis indicates that if layering is present at all, the layer thickness is comparable to the wavelength of the incident radiation, and most likely is considerably less than 0.5 μ in thickness. On the other hand, the potato starch morphology is characterized by a relatively coarse layering with few layers and considerable difference in the anisotropy between successive layers. The models for the two starches in best agreement with experimental data are as follows: almost perfectly spherulitic anisotropic structure with very thin shell-like layers—if any—for tapioca, and alternating layers of varying anisotropy several microns in thickness and probably simultaneously present with an isotropic center, for potato starch. The Vv pattern for tapioca starch is in agreement with this model, although its information content is lower owing to the experimental difficulty of recording higher order maxima. Suggestions for further morphological study of starches are presented. 相似文献
65.
The Effect of Surface Charge on the Voltage-Dependent Conductance Induced in Thin Lipid Membranes by Monazomycin 总被引:11,自引:5,他引:6
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Differences in the behavior of phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) thin lipid membranes treated with monazomycin are shown to be due to the negative surface charge on PG membranes. We demonstrate that shifts of the conductance-voltage (g-V) characteristic of PG films produced by changes of univalent or divalent cation concentrations result from changes of the membrane surface potential on one or both sides. In particular, if divalent cations are added to the aqueous phase not containing monazomycin, the resulting asymmetry of the surface potentials results in an intramembrane potential difference not recordable by electrodes in the bulk phases. Nevertheless, this intramembrane potential difference is "seen" by the monazomycin, and consequently the g-V characteristic is shifted along the voltage axis. These changes are accounted for by diffuse double layer theory. Thus we find it unnecessary to invoke specific binding of Mg++ or Ca++ to the negative charges of PG membranes to explain the observation that concentrations of these ions some 100-fold lower than that of the univalent cation present produce large shifts of the g-V characteristic. We suggest that analogous shifts of g-V characteristics in axons produced by changes of divalent cation concentration are also best explained by diffuse double layer theory. 相似文献
66.
Assay of Chick Interferons by the Inhibition of Viral Ribonucleic Acid Synthesis 总被引:8,自引:2,他引:6
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Michael McWilliams Martin S. Finkelstein Patton T. Allen David J. Giron 《Applied microbiology》1971,21(5):959-961
A method for assaying chick interferons by their inhibition of viral ribonucleic acid synthesis was devised and evaluated. The technique yielded results faster and had more flexibility than other methods with similar sensitivity and reproducibility. 相似文献
67.
Identification and quantitation of alditol acetates of neutral and amino sugars from mucins by automated gas-liquid chromatography 总被引:14,自引:0,他引:14
L J Griggs A Post E R White J A Finkelstein W E Moeckel K G Holden J E Zarembo J A Weisbach 《Analytical biochemistry》1971,43(2):369-381
An automated GLC method has been described that allows easy identification and quantitation on a single column of the alditol acetates of the commonly occurring neutral and amino sugars found in epithelial mucins on a new high-temperature stable column. Due to various losses that occur during the derivatization procedure, a constant, termed LF, must be entered into the calculation of the amount of each sugar present. When this factor is applied, the correlation between colorimetric and GLC results is very good. 相似文献
68.
Induction kinetics of the L-arabinose operon of Escherichia coli 总被引:23,自引:12,他引:11
After addition of l-arabinose to growing Escherichia coli, the l-ribulokinase (EC 2.7.1.16) and l-arabinose isomerase (EC 5.3.1.4) first appear at about 0.7 and 1.4 min, respectively. These times are consistent with the distances of the genes from the ribonucleic acid polymerase initiation site in the operon. The kinetics of appearance of these enzymes as well as those of beta-galactosidase (EC 3.2.1.23) in the same strain are consistent with a peptide elongation rate of no less than 14 amino acids per second. A measurement of the average peptide elongation rate made by measuring the kinetics of radioactive amino acid appearance in completed polypeptides yielded a rate of about 12 amino acids per s. Convenient assays of the arabinose isomerase and ribulokinase are also given. 相似文献
69.
70.
The Hedgehog (Hh) and Epidermal growth factor receptor (EGFR) signaling pathways play critical roles in pattern formation and cell proliferation in invertebrates and vertebrates. In this study, we demonstrate a direct link between these two pathways in Drosophila melanogaster. Hh and EGFR signaling are each required for the formation of a specific region of the head of the adult fruitfly. We show that hh and vein (vn), which encodes a ligand of the Drosophila EGFR (Schnepp, B., Grumbling, G., Donaldson, T. and Simcox, A. (1996) Genes Dev. 10, 2302-13), are expressed in adjacent domains within the imaginal primordium of this region. Using loss- and gain-of-function approaches, we demonstrate that Hh activates vn expression. We also show that Hh activation of vn is mediated through the gene cubitus interruptus (ci) and that this activation requires the C-terminal region of the Ci protein. Finally, we demonstrate that wingless (wg) represses vn expression, thereby limiting the domain of EGFR signaling. 相似文献