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51.
A. G. Voloshin S. Yu. Filippovich G. P. Bachurina S. G. Besaeva S. G. Ignatov 《Applied Biochemistry and Microbiology》2010,46(3):303-306
A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability
either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial
growth at a concentration above 107 cells/ml. In addition, the method allows discriminating mutants of the fungus Neurospora crassa defective in the nitrogen metabolism from the wild type strains. It is likely that nitrite reductase and nitrate reductase
enzymes regulated by the nit-2 and nit-6 genes are involved in formation of the water soluble volatile compounds of this organism. 相似文献
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I V Filippovich 《Radiobiologiia》1991,31(6):803-814
Consideration is given to various adaptive reactions to low-level radiation, their association with an absorbed dose, dose rate, radiation quality and time-interval between exposures, as well as with a cell cycle phase. Possible mechanisms of the adaptive response and the character and role of DNA damages, that can induce gene expression of the adaptive response, are discussed. The data on the influence of a preliminary long-term exposure to low-level radiation on the radiosensitivity of biological objects are analyzed with due regard for the adaptive cell response. It is concluded that the adaptive response of cells to ionizing radiation is a particular case of the phenomenon of cell adaptation to the effect of genotoxic factors of the environment. 相似文献
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Chemical inductors of differentiation were shown to cause chromatin degradation in thymus lymphocytes. This process was prevented by the protein synthesis inhibitors. The fragments formed after the effect of chemical differentiation inductors on thymocytes were fully identical to chromatin internucleosome degradation products formed in the exposed cells. Chromatin degradation under the effect of chemical differentiation inductors was most pronounced in a more radiosensitive thymocyte fraction. 相似文献
56.
It was established that the postirradiation changes in incorporation of 14C-adenine into acid-soluble and acid-insoluble fractions of thymocytes reflected cell death. When added after irradiation, nalidixic and oxolinic acids exerted a radioprotective action. The effect was absent after single washing of thymocytes incubated with these compounds for 60 min before or after irradiation. Both substances inhibited utilization of 14C-adenine and incorporation thereof into the acid-insoluble fraction of nonirradiated thymocytes and did not influence the viability of cells. 相似文献
57.
Filippovich SIu Rybakov IuA Afanas'eva TP Bachurina GP Lukina GP Ezhova IE Nosova AV Artiushkina TV Sineokiĭ SP Kritskiĭ MS 《Prikladnaia biokhimiia i mikrobiologiia》2001,37(5):554-561
The specific activity of lipoxygenase from several strains of the zygomycete Mortierella varied from 1.02 to 2.02 microMol diene per min per mg protein). The enzyme equally used linoleic or arachidonic acid as a substrate. An increase in lipoxygenase activity was observed after adding corn oil to the culture medium. Tests with inhibitors having different chemical structures revealed that the lipoxygenase activity from Mortierella cells was inhibited only by esculetin, ethanol and nordihydroguaiaretic acid (NDGA). NDGA inhibited the enzyme in vitro (IC50 = 142 microM), but its addition in the exponential phase of growth activated the enzyme. 相似文献
58.
Samsonova M. V. Min'kova N. O. Lapteva T. I. Mikodina E. V. Filippovich Yu. B. 《Russian Journal of Developmental Biology》2003,34(1):14-18
We studied the activities of the marker enzymes of physiological state and adaptive reactions, aspartate and alanine aminotransferases, in early development of the keta Oncorhynchus keta. Aspartate aminotransferase with pH optima 6.8, 7.0, 7.6, and 8.0 and alanine aminotransferase with pH optima 7.0, 7.4, 7.6, 7.8, 8.0, and 8.2 were found in the eggs, larvae, and fry. The succession of enzymes with different pH takes place during ontogenesis, as well as stage specific changes in their activity. The maximum enzymatic activity was recorded in the larvae during their rise for afloat. A correlation was established between the dynamics of enzymatic activity and soluble nitrogen and amine nitrogen contents. 相似文献
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