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971.
Mikael Flockhart Lina C. Nilsson Senna Tais Björn Ekblom William Apró Filip J. Larsen 《Cell metabolism》2021,33(5):957-970.e6
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972.
Karim Si-Tayeb Fallon K Noto Ana Sepac Filip Sedlic Zeljko J Bosnjak John W Lough Stephen A Duncan 《BMC developmental biology》2010,10(1):81
Background
The use of lentiviruses to reprogram human somatic cells into induced pluripotent stem (iPS) cells could limit their therapeutic usefulness due to the integration of viral DNA sequences into the genome of the recipient cell. Recent work has demonstrated that human iPS cells can be generated using episomal plasmids, excisable transposons, adeno or sendai viruses, mRNA, or recombinant proteins. While these approaches offer an advance, the protocols have some drawbacks. Commonly the procedures require either subcloning to identify human iPS cells that are free of exogenous DNA, a knowledge of virology and safe handling procedures, or a detailed understanding of protein biochemistry. 相似文献973.
Maes Lauranne Vervenne Thibault Van Hoof Lucas Jones Elizabeth A. V. Rega Filip Famaey Nele 《Biomechanics and modeling in mechanobiology》2023,22(5):1555-1568
Biomechanics and Modeling in Mechanobiology - The pulmonary autograft in the Ross procedure, where the aortic valve is replaced by the patient’s own pulmonary valve, is prone to failure due... 相似文献
974.
D A Filip 《Morphologie et embryologie》1984,30(3):165-173
Morphometric data on mouse atrio-ventricular valve ultrastructure are reported. The statistical analysis of the volumetric density percentage for cellular and extracellular valve components and of endothelial plasmalemmal vesicle density for the different endocardial domains (atrial, valvular and ventricular) showed: the bicuspid compared to the tricuspid valve has a more important lymphatic drainage, less vascularization, higher endothelial plasmalemmal vesicle density and more macrophages, striated muscle cells and collagen along with fewer interstitial cells, nervous terminals and elastin in the leaflet; the valvular endothelium as compared to other endocardial domains has a higher density of plasmalemmal vesicles, considering the results for both endothelial fronts (luminal and abluminal). 相似文献
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Regulation of Bacterial Cell Division: Genetic and Phenotypic Analysis of Temperature-Sensitive, Multinucleate, Filament-Forming Mutants of Escherichia coli
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Jane Smith Allen Camille C. Filip Ralph A. Gustafson Robert G. Allen James R. Walker 《Journal of bacteriology》1974,117(3):978-986
Three mutants of Escherichia coli K-12 which form filaments during 42 C incubation have been characterized. The mutant strains AX621, AX629, and AX655 continued to grow and to synthesize deoxyribonucleic acid at 42 C for 150 to 180 min, after which time growth ceased. When cultures of the mutants were transferred from 42 to 28 C, septation of the filaments began after a 25- to 30-min period and continued at a greater than normal rate until no filaments remained. Addition of chloramphenicol at the time of transfer from 42 to 28 C prevented cell division in strain AX655 and caused lysis of strains AX621 and AX629. The temperature sensitivity mutation in each strain mapped near leu. For strain AX621, the mutation was specifically located between leu and nadC by P1 transduction. Properties of these strains are compared with those of other cell division mutants. 相似文献
979.
The crude fractions of chitooligosaccharides (COS) and low-molar-mass chitosans (LMWC) were prepared by enzyme hydrolysis
of chitosan (CS). Specific growth rate of B. adolescentis, B. bifidum, B. breve, B. catenulatum, B. infantis and B. longum ssp. longum was determined in the presence of 0.025 and 0.5 % COS (<5 kDa), LMWC (5–10 kDa), and 0.025, 0.1 and 0.5 % of CS, chitosan
succinate and chitosan glutamate in vitro. Minimum inhibitory concentrations (MIC; assayed by colony counting on TPY agar plates) of COS-LMWC and CS ranged from 0.025
% to 0.75 % of CS-LMWC. The growth of all bifidobacterial strains in the presence of chitosan, its derivatives and LMWC decreased
at a concentration of 0.025 %; the bacterial growth was completely inhibited at a concentration of 0.5 %. COS did not show
any inhibitory effect, an increased growth rate was even observed in the case of B. bifidum, B. catenulatum and B. infantis. 相似文献
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