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31.
In experiments in vitro, the effects of polychromatic visible (VIS) light combined with polychromatic infrared light (VIS-IR, 480–3400 nm) and the effects of the entire spectrum of VIS radiation (385–750 nm) on viability and proliferative activity of the murine hepatoma cells MH22a were studied. In experiments in vivo, changes in the tumorigenic properties of cells MH22a were studied after the same kinds of light exposure. It was shown that irradiation of hepatoma cells with two kinds of polychromatic light at a wide range of doses (4.8–38.4 J/cm2) did not lead to an increase in the number of dead cells for 24–72 h of cultivation and did not cause deceleration of the hepatoma cell proliferation; moreover, the VIS-IR light at a dose of 4.8 J/cm2 and the VIS light at a dose 38.4 J/cm2 even promoted more intense cell proliferation after 24 h. In cells irradiated with VIS-IR and VIS light, the proliferation index rose by 1.6 and 1.4 times, respectively, and the time of the cells’ number doubling decreased as compared with control. Studying the tumorigenic properties of irradiated tumor cells has shown that, for 30 days after transplantation to syngenic mice C3HA of hepatoma cells 24 h after their irradiation with VIS-IR light at a dose of 4.8 J/cm2, the tumor volume decreased significantly (2.6–4.1 times) at all periods of observation, while the incidence of tumor formation decreased, whereas the survival of the tumor-bearing mice did not change. Transplantation of cells irradiated with the same light at a dose of 9.6 J/cm2 did not lead to significant changes in the tumor volume, the tumor formation incidence, and animal survival. The main contribution to the antitumor effect of VIS-IR light seems to be made by the VIS component, as transplantation into mice of cells irradiated with VIS light alone at a dose of 38.4 J/cm2 also stimulating proliferation of hepatoma cells in vitro resulted in a decrease of their tumorigenic properties. However, the IR component in the combined VIS-IR radiation enhanced the antitumor effect of the VIS light; as a result, it was manifested after use of doses eight times lower (4.8 J/cm2) than in the case of VIS light alone (38.4 J/cm2). Mechanisms of the decrease of tumorigenic properties of hepatoma cells after irradiation with polychromatic light at doses stimulating their proliferation in vitro are studied. 相似文献
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I. I. Tiuriaeva N. A. Filatova Yu. M. Rozanov S. Iu. Demin G. I. Blinova V. A. Ivanov 《Cell and Tissue Biology》2010,4(5):500-510
This work studies the mechanisms of dysdifferentiation at cell neoplastic transformation based on the example of heterogeneity
of the cell populations that form malignant tumors. Two natural fractions of Zajdela rat hepatoma cells are revealed that
differ in the type of growth in the primary culture. Cells of one fraction are attached to substrate and are growing in monolayer
(S-fraction), whereas cells of the other fraction are floating in the culture medium (F-fraction). Using the method of supravital
observation of the primary culture cells (of 1–2 passages) at the limit of resolution of DIC microscopy, it has been established
that both fractions contain cells of several types. Some of these cells are specific to one of the fractions and others are
present in both fractions, but with different frequencies. Using the same method, it has been shown that, at the long-term
separate cultivation of the fractions in vitro (more than 50 passages), both the cell composition and the initial ratio of
cells of different types are changed in both of them. According to the data of flow DNA cytometry, cells of both fractions
are hypotetraploid and have insignificant differences in the amount of DNA. After adaptation to conditions of cultivation
in vitro, S-fraction cells have been found to have elevated proliferative activity compared to the cells of F-fractions; after
long cultivation, the fractions already differ significantly (2.3 times) by this criterion. The content of the cell surface
laminin, a marker of hepatocellular carcinomas, is higher on cells of the F-fraction than on those of the S-fraction. The
interfraction differences are confirmed by immunologic estimations of the resistance of hepatoma cells to lyses of natural
killer cells; cells of the S-fraction of the primary culture are 2.4 times more sensitive than cells of the F-fraction, while,
after long-term cultivation, cells of the F-fraction become almost resistant to the cytotoxic action of natural killer cells.
Based on the obtained data, the most probable pathways of the dysdifferentiation of rat hepatocytes upon the establishment
of Zajdela hepatoma and at the long-term cultivation of cells of this tumor in vitro are discussed. 相似文献
35.
Tatiana S. Filatova Nikolay Naumenko Pavel A. Galenko-Yaroshevsky Denis V. Abramochkin 《Journal of physiology and biochemistry》2017,73(2):167-174
Electrophysiological effects produced by selective activation of M3 cholinoreceptors were studied in isolated left atrium preparations from rat using the standard sharp glass microelectrode technique. The stimulation of M3 receptors was obtained by application of muscarinic agonist pilocarpine (10?5 M) in the presence of selective M2 antagonist methoctramine (10?7 M). Stimulation of M3 receptors induced marked reduction of action potential duration by 14.4 ± 2.4% and 16.1 ± 2.5% of control duration measured at 50 and 90% of repolarization, respectively. This effect was completely abolished by selective M3 blocker 4-DAMP (10?8 M). In isolated myocytes obtained from the rat left atrium, similar pharmacological stimulation of M3 receptors led to suppression of peak L-type calcium current by 13.9 ± 2.6% of control amplitude (measured at +10 mV), but failed to affect K+ currents I to, I Kur, and I Kir. In the absence of M2 blocker methoctramine, pilocarpine (10?5 M) produced stronger attenuation of I CaL and induced an increase in I Kir. This additive inward rectifier current could be abolished by highly selective blocker of Kir3.1/3.4 channels tertiapin-Q (10?6 M) and therefore was identified as I KACh. Thus, in the rat atrial myocardium activation of M3 receptors leads to shortening of action potentials via suppression of I CaL, but does not enhance the major potassium currents involved in repolarization. Joint stimulation of M2 and M3 receptors produces stronger action potential shortening due to M2-mediated activation of I KACh. 相似文献
36.
Filatova EV Egorov AIu Kucher EO Kulagina KO 《Zhurnal evoliutsionno? biokhimii i fiziologii》2011,47(5):404-410
Individual characteristics of animal behavior can serve a prognostic parameter of predisposition to use of alcohol. The goal of the work was to study dynamics of formation of preference of alcohol at early stages in the process of forced alcoholization in male and female rats as well as the accompanying changes in behavior parameters. For 3 months, the rats were submitted to the forced alcoholization with 10 % ethanol. Each week the rats were tested in the "two-glass trial". Individual peculiarities of all animals were evaluated prior to, after 6 weeks, and after the end of the experiment with aid of the "open field test" and "Suok-test". Results showed that the male rats demonstrating by the end of the experiment the significantly higher level of the alcohol preference, at the initial stages of the forced alcoholization demonstrated the significantly lower preference as compared with the remaining ones. These rats also showed the lower levels of the motor and exploratory activities before alcoholization as compared with control. On the contrary, the individuals that by the end of the experiment did not differ from control by the level of the alcohol preference demonstrated prior to alcoholization in the "Suok-test" the higher anxiety level. In female groups, no statistically significant differences were observed both in parameters of the motor and exploratory activities and in the anxiety level in both tests. Thus, in male rats, the prognostic parameter predicting formation of the abuse can serve the degree of alcohol preference at the initial stages. 相似文献
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38.
A. V. Kamynina M. P. Filatova D. O. Koroev A. Y. Abramov O. M. Volpina 《Russian Journal of Bioorganic Chemistry》2013,39(2):113-120
Molecular mechanisms of β-amyloid toxic effect on brain cells during Alzheimer’s disease is associated with oxidative stress, intracellular Ca2+ increase in neurons and astrocytes and activation of reactive oxygen species production. Prion protein is known to be involved in beta-amyloid toxicity. Here we investigated an effect of affinity purified antibodies to synthetic fragment 95–123 of the prion protein (PrP-(95–123)) on β-amyloid induced cell death, Ca2+-signal, reactive oxygen species production and caspase 3 activation. We have shown that antibodies to PrP-(95–123) are able to protect neurons and astrocytes from beta-amyloid induced cell death with no effect on the intracellular Ca2+ concentration altered by beta-amyloid treatment. However, the antibodies significantly reduced β-amyloid induced reactive oxygen species production in astrocytes and decreased caspase 3 activation in neurons and astrocytes. Thus, induction of antibodies to PrP-(95–123) of the prion protein provides a new approach to anti-Alzheimer’s disease vaccine design. 相似文献
39.
L P Troitskaia L S Filatova V S Popova G N Pleskovskaia V A Nasonova 《Tsitologiia》1987,29(11):1226-1232
A study was made of the ultrastructure and polypeptide composition of liver cell nuclear matrix of F1NZB/NZW hybrid mice imitating human systemic Lupus erythematosus. Electron microscopy reveals enlargement in fibrous lamina diameter and increase in pore complex density up to the age of 8-9 months. In the terminal stages of the disease (12-13 months of age) a gradual attenuation of the intranuclear matrix and disappearance of pore complexes is observed along with a segregation and subsequent fragmentation of residual nucleoli which results eventually in the general degradation of the nuclear matrix. 30-35 polypeptide bands with molecular weight from 200 to 10 kD are revealed in polyacrylamide gel electrophoresis of the liver cell nuclear matrix of hybrid mice. Several protein bands in the high molecular weight region of 200-150 kD are strongly enhanced, and a triplet with molecular weight 70-60 kD is distinctly visible. The results obtained are interpreted as an indication of a protecting cellular reaction against antinuclear autoantibodies in the earlier stages, and a degradation of the nuclear matrix in terminal stages of the disease. It is supposed that the electron microscopic and electrophoretic patterns of the nuclear matrix indicate an accumulation of collagenous proteins. 相似文献
40.
A K Mirakhmedov P Mirkhamidova G T Shamsutdinova L S Filatova D Kh Khamidov I B Zbarski? 《Tsitologiia》1992,34(6):3-12
Morphological and biochemical investigations of pregnant rats and embryo liver cell nuclei after in vivo irradiation in the doses of 1 and 2 Gr revealed their high radiosensitivity at all stages of gestation and embryonal development. At damaging effect of radiation, we managed to observe sharp accumulation of products of lipid peroxide oxidation and suppression of the activities of such enzymes as cytochrome-c-oxidase, NAD.N-cytochrome-c-reductase, ATPase and RNAase in liver nuclei of pregnant rats and embryos. The changes of such a kind are shown to intensify with the increasing of irradiation doses. The most profound inhibition of the activities of these enzymes in liver nuclei of embryos irradiated in utero was observed during the period of organogenesis (the 13th day of the development) and in fetal period of embryogenesis (the 17th day of the development), as well as at the 13th and 17th day of gestation. The morphological data also demonstrate the high level of cell nucleus sensitivity to the action of radiation during gestation and embryogenesis. 相似文献