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排序方式: 共有255条查询结果,搜索用时 218 毫秒
211.
Flávia R Rocha Flávia S Papini-Terzi Milton Y Nishiyama Jr Ricardo ZN Vêncio Renato Vicentini Rodrigo DC Duarte Vicente E de Rosa Jr Fabiano Vinagre Carla Barsalobres Ane H Medeiros Fabiana A Rodrigues Eugênio C Ulian Sônia M Zingaretti João A Galbiatti Raul S Almeida Antonio VO Figueira Adriana S Hemerly Marcio C Silva-Filho Marcelo Menossi Gláucia M Souza 《BMC genomics》2007,8(1):1-22
212.
Esther Reefman Marcelus CJM de Jong Hilde Kuiper Marcel F Jonkman Pieter C Limburg Cees GM Kallenberg Marc Bijl 《Arthritis research & therapy》2007,8(6):R156
Apoptotic cells are thought to play an essential role in the pathogenesis of systemic lupus erythematosus (SLE). We hypothesise
that delayed or altered clearance of apoptotic cells after UV irradiation will lead to inflammation in the skin of SLE patients.
Fifteen SLE patients and 13 controls were irradiated with two minimal erythemal doses (MEDs) of ultraviolet B light (UVB).
Subsequently, skin biopsies were analysed (immuno)histologically, over 10 days, for numbers of apoptotic cells, T cells, macrophages,
and deposition of immunoglobulin and complement. Additionally, to compare results with cutaneous lesions of SLE patients,
20 biopsies of lupus erythematosus (LE) skin lesions were analysed morphologically for apoptotic cells and infiltrate. Clearance
rate of apoptotic cells after irradiation did not differ between patients and controls. Influx of macrophages in dermal and
epidermal layers was significantly increased in patients compared with controls. Five out of 15 patients developed a dermal
infiltrate that was associated with increased epidermal influx of T cells and macrophages but not with numbers of apoptotic
cells or epidermal deposition of immunoglobulins. Macrophages were ingesting multiple apoptotic bodies. Inflammatory lesions
in these patients were localised near accumulations of apoptotic keratinocytes similar as was seen in the majority of LE skin
lesions. In vivo clearance rate of apoptotic cells is comparable between SLE patients and controls. However, the presence of inflammatory
lesions in the vicinity of apoptotic cells, as observed both in UVB-induced and in LE skin lesions in SLE patients, suggests
that these lesions result from an inflammatory clearance of apoptotic cells. 相似文献
213.
Ristow P Bourhy P da Cruz McBride FW Figueira CP Huerre M Ave P Girons IS Ko AI Picardeau M 《PLoS pathogens》2007,3(7):e97
Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species. 相似文献
214.
Background
Experimental populations of Escherichia coli have evolved for 20,000 generations in a uniform environment. Their rate of improvement, as measured in competitions with the ancestor in that environment, has declined substantially over this period. This deceleration has been interpreted as the bacteria approaching a peak or plateau in a fitness landscape. Alternatively, this deceleration might be caused by non-transitive competitive interactions, in particular such that the measured advantage of later genotypes relative to earlier ones would be greater if they competed directly. 相似文献215.
Giulio Galla Gianni Barcaccia Angelo Ramina Silvio Collani Fiammetta Alagna Luciana Baldoni Nicolò GM Cultrera Federico Martinelli Luca Sebastiani Pietro Tonutti 《BMC plant biology》2009,9(1):128-17
Background
Olea europaea L. is a traditional tree crop of the Mediterranean basin with a worldwide economical high impact. Differently from other fruit tree species, little is known about the physiological and molecular basis of the olive fruit development and a few sequences of genes and gene products are available for olive in public databases. This study deals with the identification of large sets of differentially expressed genes in developing olive fruits and the subsequent computational annotation by means of different software. 相似文献216.
217.
Garcia AA Kido EA Meza AN Souza HM Pinto LR Pastina MM Leite CS Silva JA Ulian EC Figueira A Souza AP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(2):298-314
Sugarcane (Saccharum spp.) is a clonally propagated outcrossing polyploid crop of great importance in tropical agriculture. Up to now, all sugarcane
genetic maps had been developed using either full-sib progenies derived from interspecific crosses or from selfing, both approaches
not directly adopted in conventional breeding. We have developed a single integrated genetic map using a population derived
from a cross between two pre-commercial cultivars (‘SP80-180’ × ‘SP80-4966’) using a novel approach based on the simultaneous
maximum-likelihood estimation of linkage and linkage phases method specially designed for outcrossing species. From a total
of 1,118 single-dose markers (RFLP, SSR and AFLP) identified, 39% derived from a testcross configuration between the parents
segregating in a 1:1 fashion, while 61% segregated 3:1, representing heterozygous markers in both parents with the same genotypes.
The markers segregating 3:1 were used to establish linkage between the testcross markers. The final map comprised of 357 linked
markers, including 57 RFLPs, 64 SSRs and 236 AFLPs that were assigned to 131 co-segregation groups, considering a LOD score
of 5, and a recombination fraction of 37.5 cM with map distances estimated by Kosambi function. The co-segregation groups
represented a total map length of 2,602.4 cM, with a marker density of 7.3 cM. When the same data were analyzed using JoinMap
software, only 217 linked markers were assigned to 98 co-segregation groups, spanning 1,340 cM, with a marker density of 6.2 cM.
The maximum-likelihood approach reduced the number of unlinked markers to 761 (68.0%), compared to 901 (80.5%) using JoinMap.
All the co-segregation groups obtained using JoinMap were present in the map constructed based on the maximum-likelihood method.
Differences on the marker order within the co-segregation groups were observed between the two maps. Based on RFLP and SSR
markers, 42 of the 131 co-segregation groups were assembled into 12 putative homology groups. Overall, the simultaneous maximum-likelihood
estimation of linkage and linkage phases was more efficient than the method used by JoinMap to generate an integrated genetic
map of sugarcane.
E.A. Kido, A.N. Meza and H.M.B. Souza contributed equally to this work. 相似文献
218.
Eliseu S. Figueira Filho Lúcio F. A. Figueiredo Damares C. Monte-Neshich 《Plant cell reports》1994,13(12):666-670
Summary In search of establishing a system for genetic transformation of Brazilian potato cultivars, Agrobacterium tumefaciens carrying the plasmid pGV1040, was used to transform leaf discs of three cultivars of local importance, i.e., Aracy, Baronesa and Mantiqueira. This plasmid contains marker genes for resistance to kanamycin and phosphinothricin plus the gene for the enzyme -glucuronidase. A two step regeneration/selection procedure produced shoots of potato cultivar Mantiqueira with in vitro resistance to kanamycin and to phosphinothricin. After transfer to the greenhouse, the potentially transgenic plants, sprayed with the herbicide Finale® (20% a.i.; Hoechst®) remained green as compared to control clones that died immediately afterwards. Southern blot analysis and histochemical and fluorimetric assay for -glucuronidase indicated that the gene coding for the enzyme was integrated in the potato genome and could be expressed in potato tissues. No success was obtained for transformation of cultivars Aracy and Baronesa using this procedure.Abbreviations NAA
Naphthalene Acetic Acid
- BAP
Benzyl-aminopurine
- GA3
Gibberellic Acid
- PPT
Phosphinothricin
- PAT
Phosphinothricin Acetyl Transferase 相似文献
219.
Will F. Figueira Sean J. Lyman Larry B. Crowder Gil Rilov 《Environmental Biology of Fishes》2008,81(3):297-311
The demographic responses of reef fish to their environment can be complex and in many cases, quite strong. Growth, mortality,
longevity, and even reproductive effort have been demonstrated to vary for the same species of reef fish over scales of 100s
to 1,000s of kilometers due to physiological and ecological interactions. Though few studies have explicitly documented it,
this sort of habitat-mediated demography can also exist at very local scales. Here we present the results of a 2-year study
of the bicolor damselfish, Stegastes partitus, in the Florida Keys, USA. We measured density and distribution, calculated key demographic rates (growth, survival, and
fecundity), and characterized the environment (resident fish assemblage, substrate type and complexity, and food availability)
of populations living in two adjacent but different habitats, the continuous fore reef and patchy back reef. Fish on the fore
reef had an elevated growth rate and asymptotic size, increased mortality, and higher fecundity than fish on the back reef.
We identified four potential causative mechanisms for these differences: food availability; competition; intraspecific density-dependent
effects; and predation risk. Our data did not support an effect of either food availability or intraspecific density-dependence,
but rather suggested that demographic responses are affected by both competition and predation risk. 相似文献
220.
Moniliophthora perniciosa is the causal agent of the witches' broom disease of cacao. Based on available genomic sequences, we identified 30 new microsatellite loci, which were analysed using 50 isolates from four populations sampled over a wide geographical area in Brazil, including three populations from the Amazon, the fungal putative centre of diversity, plus one from Bahia. Nine loci were polymorphic, with an average of 2.9 alleles per locus. The level of polymorphism observed was low, but these markers may allow the evaluation of pathogen diversity and the establishment of molecular standards for isolate fingerprinting to support cacao breeding. 相似文献