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排序方式: 共有210条查询结果,搜索用时 31 毫秒
31.
Michael J. Rader Leonard A. Brennan Kyle A. Brazil Fidel Hernández Nova J. Silvy 《The Journal of wildlife management》2011,75(1):61-70
Nest predation is thought to be one of the major factors limiting northern bobwhite (Colinus virginianus) populations. We examined the relative impact of altering nest-predation rate, nesting habitat, and weather (i.e., temp and precipitation) on northern bobwhite population dynamics in a hypothetical 15,000-ha subtropical-rangeland ecosystem in south Texas using a simulation model. The systems model consisted of a 3-stage (i.e., eggs, juv, and ad) bobwhite population with dynamics influenced by variables affecting production, recruitment, nest predation, and mortality. We based model parameters on data collected from a 3-yr nest-predator study employing infrared-camera technology, from ongoing field research using a radio-marked population of wild bobwhites, and from the literature. The baseline simulated bobwhite population dynamics corresponded closely to empirical data, with no difference between medians of simulated (n = 30 yr) and observed bobwhite age ratios over a 28-yr period. Similarly, a time-series comparison of simulated and observed age ratios showed most (89%) observed values fell within the 5th and 95th percentiles of the simulated data over the 28-yr period. We created simulated population scenarios representing 1) baseline historical conditions, 2) predator control, 3) low precipitation, 4) low precipitation with predator control, 5) high temperature, 6) high temperature with predator control, 7) reduced nest-clump availability, and 8) reduced nest-clump availability with predator control that resulted in considerably different median bobwhite densities over 10 yr. For example, under simulated predator control, populations increased by about 55% from the baseline scenario, whereas under simulated reduced nest-clump availability, populations decreased by about 75% from the baseline scenario. Comparisons of time-series for each scenario showed that reduced nest-clump availability, low precipitation, and high temperature reduced bobwhite densities to a larger degree compared to a natural nest predation rate. Reduced nest-clump availability resulted in the most substantial decline of simulated bobwhite densities. Simulations suggested that management efforts should focus on maintaining adequate nest-clump availability and then possibly consider nest predator control as a secondary priority. © 2010 The Wildlife Society 相似文献
32.
Carrasco-Marín E Fernández-Prieto L Rodriguez-Del Rio E Madrazo-Toca F Reinheckel T Saftig P Alvarez-Dominguez C 《The Journal of biological chemistry》2011,286(5):3332-3341
The innate immune response to Listeria monocytogenes depends on phagosomal bacterial degradation by macrophages. Here, we describe the role of LIMP-2, a lysosomal type III transmembrane glycoprotein and scavenger-like protein, in Listeria phagocytosis. LIMP-2-deficient mice display a macrophage-related defect in Listeria innate immunity. They produce less acute phase pro-inflammatory cytokines/chemokines, MCP-1, TNF-α, and IL-6 but normal levels of IL-12, IL-10, and IFN-γ and a 25-fold increase in susceptibility to Listeria infection. This macrophage defect results in a low listericidal potential, poor response to TNF-α activation signals, impaired phago-lysosome transformation into antigen-processing compartments, and uncontrolled LM cytosolic growth that fails to induce normal levels of acute phase pro-inflammatory cytokines. LIMP-2 transfection of CHO cells confirmed that LIMP-2 participates in the degradation of Listeria within phagosomes, controls the late endosomal/lysosomal fusion machinery, and is linked to the activation of Rab5a. Therefore, the role of LIMP-2 appears to be connected to the TNF-α-dependent and early activation of Listeria macrophages through internal signals linking the regulation of late trafficking events with the onset of the innate Listeria immune response. 相似文献
33.
With the proliferation of social networks and blogs, the Internet is increasingly being used to disseminate personal health information rather than just as a source of information. In this paper we exploit the wealth of user-generated data, available through the micro-blogging service Twitter, to estimate and track the incidence of health conditions in society. The method is based on two stages: we start by extracting possibly relevant tweets using a set of specially crafted regular expressions, and then classify these initial messages using machine learning methods. Furthermore, we selected relevant features to improve the results and the execution times. To test the method, we considered four health states or conditions, namely flu, depression, pregnancy and eating disorders, and two locations, Portugal and Spain. We present the results obtained and demonstrate that the detection results and the performance of the method are improved after feature selection. The results are promising, with areas under the receiver operating characteristic curve between 0.7 and 0.9, and f-measure values around 0.8 and 0.9. This fact indicates that such approach provides a feasible solution for measuring and tracking the evolution of health states within the society. 相似文献
34.
Stephen J. DeMaso Joseph P. Sands Leonard A. Brennan Fidel Hernández Randy W. Deyoung 《The Journal of wildlife management》2013,77(1):24-32
Density dependence influences northern bobwhite (Colinus virginianus) reproduction and overwinter mortality. However, the functional forms of these density-dependent relationships or the factors that influence them during the annual life cycle events of this bird are not clear. We used a systems analysis approach with a compartment model based on difference equations (Δt = 3 months) for bobwhites in South Texas to simulate population behavior using 16 different functional forms of density-dependent production and overwinter mortality. During the reproductive season, a weak linear density-dependent relationship resulted in the longest population persistence (up to 100.0 yr), whereas a reverse-sigmoid density-dependent relationship had the worst population persistence (2.5–3.5 yr). Regarding overwinter mortality, a sigmoid or weak linear density-dependent relationship and a weak linear or no density-dependent reproduction relationship had the longest population persistence (87.5–100.0 yr). Weak linear density-dependent reproduction with either sigmoid or weak linear overwinter mortality produced stable fall population trends. Our results indicated that density dependence may have a greater influence on overwinter survival of bobwhites than previously thought. Inclusion of density-dependent functional relationships that represent both density-dependent reproduction and overwinter mortality, were critical for our simulation model to function properly. Therefore, integrating density-dependent relationships for both reproductive and overwinter periods of the annual cycle of bobwhite life history events is essential for conducting realistic bobwhite population simulation analyses that can be used to test different management scenarios in an integrated and interdisciplinary manner. © 2012 The Wildlife Society. 相似文献
35.
Fidel A. Roig Jose A. Boninsegna Richard L. Holmes 《Trees - Structure and Function》1992,6(4):199-203
Summary Increment borer samples taken at breast height in Pilgerodendron uviferum (Cupressaceae) trees growing in two Chilean forest stands near latitude 42° south were used to study growth rates in diameter, basal area, and height. Radial average growth is 0.51 mm/year in Santa Luci 0.44 mm/year in the Piuchué stand; the correlation between diameter and age is 0.79 in Santa Lucía and 0.64 in Piuchué. Similar results were obtained in the basal area/age relationship. Both groups of trees have a low rate of growth compared to rates obtained in other studies. Maximum values for radial growth (culmination age) are reached at 110 years of age in Santa Lucia and 50 years in Piuchue. Growth in height, averaging 5.8 cm/year, appears to be similar in all trees analyzed; occasional differences can be attributed to understory position of some trees. Comparison of radial growth and germination of Pilgerodendron plants shows a synchronous relationship, suggesting an influence of climate on the pattern of forest regeneration. 相似文献
36.
Fidel Toldra Gavin Falkous Monica Flores David Mantle 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1996,115(4):445-450
We compared the inhibitory action of individual amino acids in vitro on the activities of alanyl-, arginyl-, leucyl- and pyroglutamyl aminopeptidases purified from human and porcine skeletal muscle tissues. The range of susceptibility to inhibition by individual amino acids (<25 mM) for different aminopeptidase types broadly paralleled that for the respective substrate specificities (in terms of relative rates of hydrolysis of amino acyl-AMC derivatives) for these enzymes. Thus, alanyl aminopeptidase (which hydrolyses a broad range of aminoacyl-AMC substrates) was inhibited by a correspondingly broad range of amino acids (although the respective ranking order of amino acids was not identical in each case), whereas pyroglutamyl aminopeptidase (which hydrolyses only pyroglutamyl AMC as substrate) was inhibited by pyroglutamic acid only. The mode of inhibition (competitive/non-competitive) varied for different enzyme types, both within and between each species. For enzymes purified from human muscle, alanyl, arginyl and leucyl aminopeptidases were inhibited by amino acids via the non-competitive mode (pyroglutamyl aminopeptidase via the competitive mode), whereas corresponding enzymes purified from porcine muscle were inhibited via the competitive mode. The data obtained indicate that the same aminopeptidase types are present in human and porcine skeletal muscle tissues, with corresponding enzymes having broadly similar assay characteristics and susceptibilities to inhibition by amino acids (although the mode of inhibition for corresponding enzymes may differ in each species). Such data obtained in vitro may prove of value in devising experimental strategies to manipulate protein turnover/muscle deposition in vivo, via inhibition of aminopeptidase action after administration of an appropriate admixture of amino acids. 相似文献
37.
IDENTIFICATION AND EXPRESSION ANALYSIS OF TWO 14‐3‐3 PROTEINS IN THE MOSQUITO Aedes aegypti,AN IMPORTANT ARBOVIRUSES VECTOR
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Abel Trujillo‐Ocampo Febe Elena Cázares‐Raga Antonio Celestino‐Montes Leticia Cortés‐Martínez Mario H. Rodríguez Fidel de la Cruz Hernández‐Hernández 《Archives of insect biochemistry and physiology》2016,93(3):143-159
The 14‐3‐3 proteins are evolutionarily conserved acidic proteins that form a family with several isoforms in many cell types of plants and animals. In invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14‐3‐3 proteins are scaffold molecules that form homo‐ or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation‐dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, proliferation, apoptosis, and cancer. Here, we describe the presence of two isoforms of 14‐3‐3 in the mosquito Aedes aegypti, the main vector of dengue, yellow fever, chikungunya, and zika viruses. Both isoforms have the conserved characteristics of the family: two protein signatures (PS1 and PS2), an annexin domain, three serine residues, targets for phosphorylation (positions 58, 184, and 233), necessary for their function, and nine alpha helix‐forming segments. By sequence alignment and phylogenetic analysis, we found that the molecules correspond to ? and ζ isoforms (Aeae14‐3‐3ε and Aeae14‐3‐3ζ). The messengers and protein products were present in all stages of the mosquito life cycle and all the tissues analyzed, with a small predominance of Aeae14‐3‐3ζ except in the midgut and ovaries of adult females. The 14‐3‐3 proteins in female midgut epithelial cells were located in the cytoplasm. Our results may provide insights to further investigate the functions of these proteins in mosquitoes. 相似文献
38.
Yann Gager Emilia Tarland Dietmar Lieckfeldt Matthieu Ménage Fidel Botero-Castro Stephen J. Rossiter Robert H. S. Kraus Arne Ludwig Dina K. N. Dechmann 《PloS one》2016,11(3)
A fundamental condition for any work with free-ranging animals is correct species identification. However, in case of bats, information on local species assemblies is frequently limited especially in regions with high biodiversity such as the Neotropics. The bat genus Molossus is a typical example of this, with morphologically similar species often occurring in sympatry. We used a multi-method approach based on molecular, morphometric and acoustic information collected from 962 individuals of Molossus bondae, M. coibensis, and M. molossus captured in Panama. We distinguished M. bondae based on size and pelage coloration. We identified two robust species clusters composed of M. molossus and M. coibensis based on 18 microsatellite markers but also on a more stringently determined set of four markers. Phylogenetic reconstructions using the mitochondrial gene co1 (DNA barcode) were used to diagnose these microsatellite clusters as M. molossus and M. coibensis. To differentiate species, morphological information was only reliable when forearm length and body mass were combined in a linear discriminant function (95.9% correctly identified individuals). When looking in more detail at M. molossus and M. coibensis, only four out of 13 wing parameters were informative for species differentiation, with M. coibensis showing lower values for hand wing area and hand wing length and higher values for wing loading. Acoustic recordings after release required categorization of calls into types, yielding only two informative subsets: approach calls and two-toned search calls. Our data emphasizes the importance of combining morphological traits and independent genetic data to inform the best choice and combination of discriminatory information used in the field. Because parameters can vary geographically, the multi-method approach may need to be adjusted to local species assemblies and populations to be entirely informative. 相似文献
39.
Aspergillus nidulans beta-tubulin genes are unusually divergent 总被引:14,自引:0,他引:14
40.