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151.
152.
Fidel J. Lopez Aparicio Isidoro Izquierdo Cubero Maria D. Portal Olea 《Carbohydrate research》1984,134(1):39-47
Hydroxylation of trans-1,3,4-trideoxy-5,6-O-isopropylidene-3-C-methyl-d-glycero-hex-3-enulose with osmium tetraoxide gave a mixture of 1-deoxy-5,6-O-isopropylidene-3-C-methyl-d-arabino- and -d-xylo-hexulose that was partially resolved by acetonation to give 1-deoxy-2,3:4,5-di-O-isopropylidene-3-C-methyl-β-d-fructopyranose (4), 1-deoxy-3,4:5,6-di-O-isopropylidene-3-C-methyl-keto-d-fructose (5), and 1-deoxy-2,3:4,6-di-O-isopropylidene-3-C-methyl-α-d-sorbofuranose (6). Treatment of a mixture of 4 and 5 with sodium borohydride gave, after column chromatography, 4 and 1-deoxy-3,4:5,6-di-O-isopropylidene-3-C-methyl-d-manno- and -d-gluco-hexitol. Deuterated derivatives corresponding to 4–6 were obtained when isopropylidenation was carried out with acetone-d6. Deacetonation of 4 and 5 yielded 1-deoxy-3-C-methyl-d-fructose, and 6 similarly afforded 1-deoxy-3-C-methyl-d-sorbose. 相似文献
153.
Kaio Cesar Simiano Tavares Ana Christina de Oliveira Dias Cícera Regina Lazzarotto Saul Gaudencio Neto Igor de Sá Carneiro Felipe Ledur Ongaratto Antônio Frederico Michel Pinto Luís Henrique de Aguiar Carlos Enrique Mendez Calderón Jorge Roberto Toledo Fidel Ovidio Castro Diogenes Santiago Santos Jocelei Maria Chies Marcelo Bertolini Luciana Relly Bertolini 《Molecular biotechnology》2016,58(1):47-55
154.
Armando Gómez Ojeda Katarzyna Wrobel Alma Rosa Corrales Escobosa Ma. Eugenia Garay-Sevilla Kazimierz Wrobel 《Analytical biochemistry》2014
Bioanalytical relevance of glyoxal (Go) and methylglyoxal (MGo) arises from their role as biomarkers of glycation processes and oxidative stress. The third compound of interest in this work is diacetyl (DMGo), a component of different food products and alcoholic beverages and one of the small α-ketoaldehydes previously reported in urine. The original idea for the determination of the above compounds by reversed phase high-performance liquid chromatography (HPLC) with fluorimetric detection was to use 4-methoxy-o-phenylenediamine (4MPD) as a derivatizing reagent and diethylglyoxal (DEGo) as internal standard. Acetonitrile was added to urine for matrix precipitation, and derivatization reaction was carried out in the diluted supernatant at neutral pH (40 °C, 4 h); after acidification, salt-induced phase separation enabled recovery of the obtained quinoxalines in the acetonitrile layer. The separation was achieved within 12 min using a C18 Kinetex column and gradient elution. The calibration detection limits for Go, MGo, and DMGo were 0.46, 0.39, and 0.28 μg/L, respectively. Within-day precision for real-world samples did not exceed 6%. Several urine samples from healthy volunteers, diabetic subjects, and juvenile swimmers were analyzed. The sensitivity of the procedure proposed here enabled detection of differences between analyte concentrations in urine from patients at different clinical or exposure-related conditions. 相似文献
155.
Enrique Santamaría María I. Mora Elvira Carro-Roldn Manuela Molina Joaquín Fernndez-Irigoyen Peggy Marconi Roberto Manservigi Anna Greco Alberto L. Epstein Jesús Prieto Rubn Hernndez-Alcoceba Fernando J. Corrales 《Journal of Proteomics》2009,73(1):153-160
Recent studies based on animal models have shown the advantages and potential of oncolytic viral therapy using HSV-1 -based replication-competent vectors in the treatment of liver tumors, but little is known about the cellular targets that are modulated during viral infection. In the present work, we have studied the effects of intratumoral injections of HSV-1 Cgal+ strain in a murine model of human hepatoma xenografts. Viral replication was assessed for more than 1 month, leading to a significant reduction of tumor growth rate mediated, in part, by a cyclin B dependent cell proliferation arrest. Early events resulting in this effect were analyzed using a proteomic approach. Protein extracts from xenografted human hepatomas treated with saline or HSV-1 Cgal+ strain during 24 h were compared by 2-D DIGE and differential spots were identified by nanoLC-ESI-MS/MS. Alterations on glutathione S transferase 1 Omega, and ERp29 suggest novel HSV-1 Cgal+ targets in solid liver tumors. Additionally, ERp29 showed a complex differential isoform pattern upon HSV-1 Cgal+ infection, suggesting regulatory mechanisms based on post-translational modification events. 相似文献
156.
Stephen J. Demaso Fidel Hernández Leonard A. Brennan Ralph L. Bingham 《The Journal of wildlife management》2011,75(3):740-746
The simple saddlepoint approximation (SSA) uses the mean, variance, and skewness (a measure of the asymmetry of the distribution) of a data set to algebraically approximate the probability density function of a selected variable. We compared habitat-suitability bounds estimated with SSAs and continuous selection functions. Habitat-suitability bounds for bobwhite nesting based on the SSA method were biologically comparable to the results of the method based on continuous selection functions. The SSA approach allows habitat-suitability bounds to be estimated using algebra and can be calculated in computer spreadsheets. © 2011 The Wildlife Society. 相似文献
157.
158.
History and new insights into host defense against vaginal candidiasis 总被引:12,自引:0,他引:12
Fidel PL 《Trends in microbiology》2004,12(5):220-227
159.
Avila MA García-Trevijano ER Lu SC Corrales FJ Mato JM 《The international journal of biochemistry & cell biology》2004,36(11):2125-2130
5'-Methylthioadenosine (MTA) is a naturally occurring sulfur-containing nucleoside present in all mammalian tissues. MTA is produced from S-adenosylmethionine mainly through the polyamine biosynthetic pathway, where it behaves as a powerful inhibitory product. This compound is metabolized solely by MTA-phosphorylase, to yield 5-methylthioribose-1-phosphate and adenine, a crucial step in the methionine and purine salvage pathways, respectively. Abundant evidence has accumulated over time suggesting that MTA can affect cellular processes in many ways. MTA has been shown to influence numerous critical responses of the cell including regulation of gene expression, proliferation, differentiation and apoptosis. Although most of these responses have been observed at the pharmacological level, their specificity makes it tempting to speculate that endogenous MTA could play a regulatory role in the cell. Finally, observations carried out in models of liver damage and cancer demonstrate a therapeutic potential for MTA that deserves further consideration. 相似文献
160.
Elizabeth Pérez José L Gallegos Leticia Cortés Karla G Calderón José C Luna Febe E Cázares María C Velasquillo Juan B Kouri Fidel C Hernández 《Proteome science》2010,8(1):27