全文获取类型
收费全文 | 157篇 |
免费 | 19篇 |
出版年
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 28篇 |
2011年 | 8篇 |
2010年 | 3篇 |
2009年 | 7篇 |
2008年 | 5篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 10篇 |
2004年 | 5篇 |
2002年 | 5篇 |
2001年 | 3篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1992年 | 2篇 |
1989年 | 3篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1926年 | 1篇 |
1925年 | 1篇 |
1924年 | 1篇 |
1921年 | 1篇 |
1920年 | 1篇 |
1915年 | 2篇 |
1914年 | 1篇 |
1909年 | 1篇 |
1902年 | 1篇 |
1901年 | 1篇 |
1897年 | 2篇 |
1892年 | 1篇 |
1891年 | 1篇 |
1889年 | 1篇 |
1888年 | 1篇 |
1887年 | 1篇 |
1883年 | 3篇 |
1882年 | 5篇 |
1881年 | 2篇 |
1880年 | 1篇 |
1879年 | 6篇 |
1878年 | 2篇 |
1877年 | 2篇 |
1876年 | 1篇 |
排序方式: 共有176条查询结果,搜索用时 15 毫秒
101.
102.
Few Ne Chew Wen Siang Tan Huey Chern Boo 《Preparative biochemistry & biotechnology》2013,43(6):535-550
An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. Three variables (agitation rate, temperature, and time of induction) exerted significant effects on functional GFP production in E. coli shake flask cultivation and were optimized subsequently using the Box–Behnken design. An agitation rate of 206 rpm at 31°C and induction of the protein expression when the cell density (OD600nm) reaches 1.04 could enhance the yield of functional GFP production from 0.025 g/L to 0.241 g/L, which is about ninefold higher than the unoptimized conditions. Unoptimized cultivation conditions resulted in protein aggregation and hence reduced the quantity of functional GFP. The model and regression equation based on the shake flask cultivation could be applied to a 2-L bioreactor for maximum functional GFP production. 相似文献
103.
HANDE GURER-ORHAN HILMI ORHAN SIBEL SUZEN M. ORHAN PÜSKÜLLÜ ERDEM BUYUKBINGOL 《Journal of enzyme inhibition and medicinal chemistry》2013,28(2):241-247
New, except 1d, melatonin analogue benzimidazole derivatives were synthesized and characterized in the present study. The potential role of melatonin as an antioxidant by scavenging and detoxifying ROS raised the possibility that compounds that are analogous to melatonin can also be used for their antioxidant properties. Therefore the antioxidant effects of the newly synthesized compounds were investigated in vitro by means of their inhibitory effect on hydrogen peroxide-induced erythrocyte membrane lipid peroxidation (EMLP) and on various erythrocyte antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and glucose-6-phosphate dehydrogenase (G6PD). The synthesized benzimidazole derivatives showed remarkable antioxidant activity in vitro in the H2O2-induced EMLP system. Furthermore their effects on various antioxidant enzymes are discussed and evaluated from the perspective of structure- activity relationships. 相似文献
104.
Frédéric D Chevalier Claudia LL Valentim Philip T LoVerde Timothy JC Anderson 《BMC genomics》2014,15(1)
Background
Identification of parasite genes that underlie traits such as drug resistance and host specificity is challenging using classical linkage mapping approaches. Extreme QTL (X-QTL) methods, originally developed by rodent malaria and yeast researchers, promise to increase the power and simplify logistics of linkage mapping in experimental crosses of schistosomes (or other helminth parasites), because many 1000s of progeny can be analysed, phenotyping is not required, and progeny pools rather than individuals are genotyped. We explored the utility of this method for mapping a drug resistance gene in the human parasitic fluke Schistosoma mansoni.Results
We staged a genetic cross between oxamniquine sensitive and resistant parasites, then between two F1 progeny, to generate multiple F2 progeny. One group of F2s infecting hamsters was treated with oxamniquine, while a second group was left untreated. We used exome capture to reduce the size of the genome (from 363 Mb to 15 Mb) and exomes from pooled F2 progeny (treated males, untreated males, treated females, untreated females) and the two parent parasites were sequenced to high read depth (mean = 95-366×) and allele frequencies at 14,489 variants compared. We observed dramatic enrichment of alleles from the resistant parent in a small region of chromosome 6 in drug-treated male and female pools (combined analysis: = 11.07, p = 8.74 × 10-29). This region contains Smp_089320 a gene encoding a sulfotransferase recently implicated in oxamniquine resistance using classical linkage mapping methods.Conclusions
These results (a) demonstrate the utility of exome capture for generating reduced representation libraries in Schistosoma mansoni, and (b) provide proof-of-principle that X-QTL methods can be successfully applied to an important human helminth. The combination of these methods will simplify linkage analysis of biomedically or biologically important traits in this parasite.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-617) contains supplementary material, which is available to authorized users. 相似文献105.
106.
107.
In 1950, Raphael Patai published his research on Venta Prieta, a Mexican town in which some residents lived as Jews despite having little knowledge of Judaism. Like other visitors, Patai was perplexed. Why did they wish to live as Jews? While Patai never answered this question to his satisfaction, he believed the answer would be found by developing a psychological profile of the residents, an approach in keeping with culture and personality theorists of the day. The present article provides a different solution. Drawing on additional sources, and short visits, we argue that Venta Prieta was not only a stop on the Jewish tourist circuit by 1950 but also developed out of a unique exchange. While U.S. Jews, as evangelical Protestants before them, provided a model for upwardly mobile Mexicans, Venta Prieta enabled middle-class tourists to experience Judaism in a pastoral setting and to "repair the world" (tikkun). [Keywords: Judaism, tourism, dialogical anthropology, social change, social mobility] 相似文献
108.
109.
110.