A gel-forming (1→3)-β-D-glucan isolated from cyttaria harioti fischer

An alkali-soluble glucan, [α]_D + 11° (M potassium hydroxide) having a degree of polymerization of 220, has been isolated from the fruit bodies of the tree fungus Cyttaria harioti Fischer. Periodate oxidation and methylation analysis show that it consists of a highly branched β-D-(1→3)-linked backbone. Hydrolysis of the methylated polysaccharide yielded 2,3,4,6-tetra-O-methyl- (24.5 mol%), 2,4,6-tri-O-methyl-(39.4 mol%), 2,3,4-tri-O-methyl- (8.6 mol%), and 2,4-di-O-methyl-D-glucose (27.5 mol%). Periodate-oxidation results substantiate the methylation studies. The general structural features of the glucan are discussed.     

Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens

An enzyme immunoassay procedure is described for the identification of major histocompatibility haplotypes and lymphocyte alloantigens in 19 inbred mouse strains. The assay was found to be useful for the genetic monitoring of such mice.     

PEATmoss (Physcomitrella Expression Atlas Tool): a unified gene expression atlas for the model plant Physcomitrella patens

Physcomitrella patens is a bryophyte model plant that is often used to study plant evolution and development. Its resources are of great importance for comparative genomics and evo‐devo approaches. However, expression data from Physcomitrella patens were so far generated using different gene annotation versions and three different platforms: CombiMatrix and NimbleGen expression microarrays and RNA sequencing. The currently available P. patens expression data are distributed across three tools with different visualization methods to access the data. Here, we introduce an interactive expression atlas, Physcomitrella Expression Atlas Tool (PEATmoss), that unifies publicly available expression data for P. patens and provides multiple visualization methods to query the data in a single web‐based tool. Moreover, PEATmoss includes 35 expression experiments not previously available in any other expression atlas. To facilitate gene expression queries across different gene annotation versions, and to access P. patens annotations and related resources, a lookup database and web tool linked to PEATmoss was implemented. PEATmoss can be accessed at https://peatmoss.online.uni-marburg.de