全文获取类型
收费全文 | 5022篇 |
免费 | 310篇 |
国内免费 | 1篇 |
出版年
2024年 | 6篇 |
2023年 | 49篇 |
2022年 | 105篇 |
2021年 | 200篇 |
2020年 | 126篇 |
2019年 | 163篇 |
2018年 | 178篇 |
2017年 | 185篇 |
2016年 | 237篇 |
2015年 | 328篇 |
2014年 | 362篇 |
2013年 | 438篇 |
2012年 | 453篇 |
2011年 | 385篇 |
2010年 | 246篇 |
2009年 | 193篇 |
2008年 | 258篇 |
2007年 | 221篇 |
2006年 | 207篇 |
2005年 | 161篇 |
2004年 | 169篇 |
2003年 | 135篇 |
2002年 | 126篇 |
2001年 | 66篇 |
2000年 | 42篇 |
1999年 | 42篇 |
1998年 | 25篇 |
1997年 | 18篇 |
1996年 | 14篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 18篇 |
1991年 | 13篇 |
1990年 | 12篇 |
1989年 | 12篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 6篇 |
1984年 | 8篇 |
1983年 | 9篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1975年 | 3篇 |
1971年 | 3篇 |
1970年 | 4篇 |
1969年 | 9篇 |
1968年 | 5篇 |
1965年 | 4篇 |
排序方式: 共有5333条查询结果,搜索用时 15 毫秒
991.
Ricardo Dias Peruca Roberta Gomes Coelho Gercina Gonçalves da Silva Hemerson Pistori Luciana Marçal Ravaglia Antonia Railda Roel Glaucia Braz Alcantara 《Arthropod-Plant Interactions》2018,12(2):257-266
As soybean (Glycine max [L.] Merrill) is an important crop throughout the world, the action of herbivorous insects responsible for economic and productivity losses is the subject of constant research. Spodoptera frugiperda (J.E. Smith) caterpillars can cause extensive damage to soybean culture; this work investigated possible harmful effects on these caterpillars associated with the possible induced defenses of soybean plants. For this purpose, we assessed the biology of the insect (leaf consumption and performance traits) and chemical composition of the soybean leaves by ultraviolet–visible spectroscopy and chemometrics of three treatments: control, mechanically wounded soybean leaves, and S. frugiperda-damaged soybean leaves. The results reveal that both types of injuries induce changes in soybean metabolism regarding the production of phenolic substances, although only the herbivore-damaged plants provoke negative effects on insect biology. Variations in carotenoid production during the circadian cycle were also found in the control group. These results confirm that the soybean plants could endure and activate chemical defense mechanisms that impair the developmental lifecycle of the insect, suggesting possibilities for sustainable control strategies. 相似文献
992.
Elis Eleutherio Aline de Araujo Brasil Mauro Braga França Diego Seixas Gomes de Almeida Germana Breves Rona Rayne Stfhany Silva Magalhães 《Fungal biology》2018,122(6):514-525
The yeast Saccharomyces cerevisiae has played a vital role in the understanding of the molecular basis of aging and the relationship of aging process with oxidative stress (non-homeostatic accumulation of Reactive Oxygen Species, ROS). The mammalian and yeast antioxidant responses are similar and over 25 % of human-degenerative disease related genes have close homologues in yeast. The reduced genetic redundancy of yeast facilitates visualization of the effect of a deleted or mutated gene. By manipulating growth conditions, yeast cells can survive only fermenting (low ROS levels) or respiring (increased ROS levels), which facilitates the elucidation of the mechanisms involved with acquisition of tolerance to oxidative stress. Furthermore, the yeast databases are the most complete of all eukaryotic models. In this work, we highlight the value of S. cerevisiae as a model to investigate the oxidative stress response and its potential impact on aging and age-related diseases. 相似文献
993.
Solange Oliveira Juliana Aizawa Herbert Sousa Soares Daniela Pontes Chiebao Márcio Botelho de Castro Aline S. Hora Marcos Gomes Lopes Gereon Schares Mark C. Jenkins Oliver C.H. Kwok Ricardo Augusto Dias Solange Maria Gennari Jitender P. Dubey Hilda Fátima Jesus Pena 《International journal for parasitology》2018,48(2):117-123
The importance of birds in the biological cycle of Neospora caninum is not clear. We report unsuccessful Neospora infection in chickens (Gallus gallus domesticus) using two isolates of N. caninum. In experiment #1, 30 White Leghorn chickens were orally inoculated with viable N. caninum oocysts (NC-SP1 isolate, 200 oocysts per bird) via the crop at 21 days of age. Groups of three birds were euthanised at intervals of 7 days (a total of 9 weeks) and one group was challenged with the same oocyst dose at 37 days p.i. and observed for 11 weeks. Blood samples were collected weekly, and sera were tested using IFAT. Chicken tissues were collected for PCR, quantitative PCR and immunohistochemistry. Two dogs approximately 45 days of age were fed with tissues from chickens euthanised at 138 and 159 days p.i. The results indicated that the chickens were resistant to neosporosis as revealed by failure to seroconvert, to detect parasite DNA or N. caninum antigen by immunohistochemistry in inoculated bird tissues, and by no oocyst excretion by the dogs fed avian tissues. Similar results were obtained in experiment #2, in which 34 1-week-old chickens were each s.c. inoculated with 100,000 tachyzoites of the NcWTDMn1 isolate of N. caninum. The chickens were euthanised on days 7, 15, 22, 28, 36 and 60 p.i. At necropsy, all tissues and serum from each bird were collected. All chickens remained asymptomatic, and N. caninum antigen was not detected by immunohistochemistry. Seven chickens euthanised at day 60 p.i. demonstrated low (1:25 dilution) levels of antibodies by using the Neospora agglutination test. Two 12-week-old dogs fed tissues pooled from 10 inoculated chickens euthanised at day 60 p.i. did not excrete N. caninum oocysts. This investigation indicates that chickens are resistant to experimental infection by N. caninum. 相似文献
994.
PDGFB as a vascular normalization agent in an ovarian cancer model treated with a gamma‐secretase inhibitor
下载免费PDF全文
![点击此处可从《Journal of cellular physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
995.
Environmental gradients and competition influence aquatic macrophyte distribution in estuaries. The competition-to-stress hypothesis states that some species are excluded from lower estuaries (high salinity) due to abiotic stress and others from upper estuaries (low salinity) by competition. The growth of Crinum americanum L. and Spartina alterniflora Loisel. in monoculture (10:0/0:10) and mixed culture (5:5) under different salinity levels (4/12/26) was analysed by a laboratory experiment (3 cultures × 3 sediment types × 3 replicate) to understand the role of competition and salinity on the distribution of these species in a tropical estuary as well as to verify whether the competition-to-stress hypothesis explains their zonation. We tested the hypothesis that S. alterniflora is not established in the upper estuary due to the effect of competition with C. americanum, whereas the latter presents restrictions to high salinity and has greater competitive ability in the upper estuary. Our data confirm the competition-to-stress hypothesis but not as proposed originally. We conclude that abiotic stress (low nutrient availability) is responsible for the absence of S. alterniflora in the upper estuary and that the competition between the two species is responsible for the absence of C. americanum in the lower estuary. 相似文献
996.
Cumulative ecological effects of a Neotropical reservoir cascade across multiple assemblages 总被引:2,自引:0,他引:2
Natália Carneiro Lacerda dos Santos Emili García-Berthou Juliana Déo Dias Taise Miranda Lopes Igor de Paiva Affonso William Severi Luiz Carlos Gomes Angelo Antonio Agostinho 《Hydrobiologia》2018,806(1):77-93
Kettle holes are often abundant within agriculturally used moraine landscapes. They are highly enriched with nutrients and considered hotspots of carbon turnover. However, data on their primary productivity remain rare. We analysed two kettle holes typical to Germany with common aquatic plant communities during one year. We hypothesised that gross primary production (GPP) rates would be high compared to other temperate freshwater ecosystems, leading to high sediment deposition. Summer GPP rates (4.5–5.1 g C m?2 day?1) were higher than those of most temperate freshwater systems, but GPP rates were reduced by 90% in winter. Macrophytes dominated GPP from May to September with emergent macrophytes accounting for half of the GPP. Periphyton contributed to most of the system GPP throughout the rest of the year. Sediment deposition rates were high and correlated with GPP in one kettle hole. In contrast, due to prolonged periods of anoxia, aerobic sediment mineralisation was low while sediment phosphorus release was significant. Our results suggest that kettle holes have a high potential for carbon burial, provided they do not fully dry up during warm years. Due to their unique features, they should not be automatically grouped with ponds and shallow lakes in global carbon budget estimates. 相似文献
997.
Luis?Carlos?MartínezEmail authorView authors OrcID profile
return OK on get Angelica?Plata-Rueda Guilherme?da?Silva Neves Jamile?Fernanda?Cossolin Marcelo?Henrique?dos?Santos José?Cola?Zanuncio José?Eduardo?Serr?o 《Protoplasma》2018,255(6):1693-1702
The osmeterium, found in papilionoid larvae, is an eversible organ with an exocrine gland that produces substances in response to the mechanical disturbances caused by natural enemies. The anatomy, histology and ultrastructure of the osmeterium, and the chemical composition of its secretion in Heraclides thoas (Lepidoptera: Papilionidae) were studied. Heraclides thoas larvae have a Y-shaped osmeterium in the thorax. The surface of the osmeterium has a rough cuticle lining cells with papillae and irregular folds, whereas the cells that limited the gland pores are irregular, folded, and devoid of papillae. Two types of cells are found: (i) cuticular epidermal cells on the surface of the tubular arms of the osmeterium and (ii) secretory cells of the ellipsoid gland within the region of the glandular pore. Cuticular epidermal cells show a thick cuticle, with several layers divided into epicuticle and lamellar endocuticle. Secretory cells are polygonal, with extensive folds in the basal plasma membrane that formed extracellular channels. The cytoplasm has mitochondria, ribosomes, and numerous vacuoles, whereas the nucleus is irregular in shape with decondensed chromatin. The chemical composition of the osmeterial secretion comprised (Z)-α-bisabolene (25.4%), α-bisabol (20.6%), β-bisabolene (13.1%), (E)-α-bisabolene 8%), β-pinene (9.91%), longipinene epoxide (8.92%), (Z)-β-farnesene (6.96%), β-caryophyllene (2.05%), farnesol (1.86%), linalyl propionate (1.86%), and 1-octyn-4-ol (1.07%). The morphological features suggest that the cuticular epidermal cells play a major role in the maintenance and protection of the osmeterium, whereas secretory cells are responsible for production of osmeterial secretions. 相似文献
998.
Ana?Carvalho Fernanda?Leal Manuela?Matos José?Lima-BritoEmail authorView authors OrcID profile
return OK on get 《Protoplasma》2018,255(6):1725-1740
Grapevine varieties respond differentially to heat stress (HS). HS ultimately reduces the photosynthesis and respiratory performance. However, the HS effects in the leaf nuclei and mitotic cells of grapevine are barely known. This work intends to evaluate the HS effects in the leaf mitotic cell cycle and chromosomes of four wine-producing varieties: Touriga Franca (TF), Touriga Nacional (TN), Rabigato, and Viosinho. In vitro plants with 11 months were used in a stepwise acclimation and recovery (SAR) experimental setup comprising different phases: heat acclimation period (3 h—32 °C), extreme HS (1 h—42 °C), and two recovery periods (3 h—32 °C and 24 h—25 °C), and compared to control plants (maintained in vitro at 25 °C). At the end of each SAR phase, leaves were collected, fixed, and used for cell suspensions and chromosome preparations. Normal and abnormal interphase and mitotic cells were observed, scored, and statistically analyzed in all varieties and treatments (control and SAR phases). Different types of chromosomal anomalies in all mitotic phases, treatments, and varieties were found. In all varieties, the percentage of dividing cells with anomalies (%DCA) after extreme HS increased relative to control. TF and Viosinho were considered the most tolerant to HS. TF showed a gradual MI reduction from heat acclimation to HS and the lowest %DCA after HS and 24 h of recovery. Only Viosinho reached the control values after the long recovery period. Extrapolating these data to the field, we hypothesize that during consecutive hot summer days, the grapevine plants will not have time or capacity to recover from the mitotic anomalies caused by high temperatures. 相似文献
999.
Fernanda Pozzan Paim Kim Valenta Colin A. Chapman Adriano Pereira Paglia Helder Lima de Queiroz 《Primates; journal of primatology》2018,59(4):395-404
Integration between ecology and biogeography provides insights into how niche specialization affects the geographical distribution of species. Given that rivers are not effective barriers to dispersal in three parapatric species of squirrel monkeys (Saimiri vanzolinii, S. cassiquiarensis and S. macrodon) inhabiting floodplain forests of Central Amazonia, we tested whether forest structure and tree diversity may explain species differences in niche specialization and spatial segregation. We sampled 6617 trees of 326 species in three habitats (high várzea, low várzea and chavascal) used by three Saimiri species, and estimated tree species richness in each of them. For each tree, we measured variables known to influence habitat use in primates, such as crown area and presence of lianas, epiphytes and hemi-epiphytes. We used ANOVA to compare these variables and performed multivariate analyses (NMDS, ANOSIM and SIMPER) to evaluate dissimilarities in forest structure among each habitat inhabited by the three Saimiri species. We identified differences in the tree species richness, crown area and presence of lianas, epiphytes and hemi-epiphytes between the three habitats for all Saimiri species. NMDS demonstrated that areas of high and low várzeas occupied by S. vanzolinii were clearly separated from the other species. We also found that different plant species contributed to dissimilarity among Saimiri ranges. Our findings support the hypothesis that tree community structure may promote niche specialization and spatial segregation among primates. We discuss how these patterns could have been favored by historical changes in forest flood patterns, the evolutionary history of Saimiri spp., and past competition. 相似文献
1000.
The influence of environmental features in the content of mycosporine‐like amino acids in red marine algae along the Brazilian coast
下载免费PDF全文
![点击此处可从《Journal of phycology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bruno Briani Marina N. Sissini Leidson A. Lucena Manuela B. Batista Iara O. Costa José M. C. Nunes Caroline Schmitz Fernanda Ramlov Marcelo Maraschin Nathalie Korbee Leonardo Rörig Paulo A. Horta Félix L. Figueroa José B. Barufi 《Journal of phycology》2018,54(3):380-390
Mycosporine‐like amino acids (MAA) are ultraviolet screen substances synthesized by marine algae. The physiological function of these substances is related to cellular protection against UV radiation and as a protective mechanism against oxidative stress. These substances can be found mainly in the ocean, among red seaweeds. Its concentration in organisms has been related to ultraviolet radiation and availability of inorganic nitrogen in the environment. We start our study of MAA content in different species to understand if environmental conditions influence the concentration of MAAs in red seaweeds. The Brazilian coast presents abiotic factors that interact to create different physical‐chemical features in the environment. We collected 441 samples from 39 species of red seaweed easily found in the intertidal zone, in low tide, during the summer of 2015. The sampling encompassed a latitudinal gradient (3° S to 28°5′ S) at 23 points along the coast. We quantified and identified the content of MAAs in species through the method of high performance liquid chromatography. We detected for the first time the occurrence of MAAs in certain species of red algae that have not been reported to contain MAAs before. We confirmed that some environmental factors influenced the content of MAAs. Enhanced MAA contents, for example, were found in environments with a basic pH, a high ultraviolet index, and high concentrations of phosphate and nitrate. Salinity, dissolved oxygen and variations of sea surface temperature also influenced, in a secondary way, MAA content in algae in their natural environments. 相似文献