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排序方式: 共有425条查询结果,搜索用时 15 毫秒
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Andrei Sakai Ana Paula de Sousa Mesquita Erica Valadares de Castro Levatti Anita Hilda Straus Helena B. Nader Carla Cristina Lopes Luciano Caseli 《生物化学与生物物理学报:生物膜》2019,1861(10):182992
Trastuzumab (Tmab) is a monoclonal antibody administered as targeted therapy for HER2-positive breast cancer whose molecular interactions at the HER2 receptor microenvironment are not completely clarified yet. This paper describes the influence of Tmab in the molecular organization of films of biological-relevant molecules at the air water interface. For that, we spread components of tumorigenic and non-tumorigenic cells directly on the air-water interface. The physicochemical properties of the films were investigated with surface pressure-area isotherms and Brewster angle microscopy, and distinction between the cellular lines with higher or lower amount of HER2 could be detected based on the physicochemical properties of the interfacial films. The systems organized at the air-water interface were transferred to solid supports as Langmuir-Blodgett films and the nano-scale morphology investigated with atomic force microscopy. The overall results related to Tmab interacting with the films lead to the conclusion that Tmab tends to condense rich-HER2 films, causing irregular dimerization of the receptor protein, changing the membrane topography of the films, with formation of phases with different levels of reflectivity and aggregation morphology, and finally revealing that the interaction of the antibody with proteo-lipidic biointerfaces is modulated by the film composition. We believe that novel perspectives concerning the molecular interactions in the plasma membrane microenvironment through Langmuir monolayers can be obtained from this work in order to enhance the Tmab-based cancer therapy. 相似文献
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Factors associated with high hospital resource use in a population‐based study of children with orofacial clefts 下载免费PDF全文
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Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells
Dimitri Conomos Michael D. Stutz Mark Hills Axel A. Neumann Tracy M. Bryan Roger R. Reddel Hilda A. Pickett 《The Journal of cell biology》2012,199(6):893-906
Telomeres in cells that use the recombination-mediated alternative lengthening of telomeres (ALT) pathway elicit a DNA damage response that is partly independent of telomere length. We therefore investigated whether ALT telomeres contain structural abnormalities that contribute to ALT activity. Here we used next generation sequencing to analyze the DNA content of ALT telomeres. We discovered that variant repeats were interspersed throughout the telomeres of ALT cells. We found that the C-type (TCAGGG) variant repeat predominated and created a high-affinity binding site for the nuclear receptors COUP-TF2 and TR4. Nuclear receptors were directly recruited to telomeres and ALT-associated characteristics were induced after incorporation of the C-type variant repeat by a mutant telomerase. We propose that the presence of variant repeats throughout ALT telomeres results from recombination-mediated telomere replication and spreading of variant repeats from the proximal regions of the telomeres and that the consequent binding of nuclear receptors alters the architecture of telomeres to facilitate further recombination. 相似文献
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Twan van den Beucken Marianne Koritzinsky Hanneke Niessen Ludwig Dubois Kim Savelkouls Hilda Mujcic Barry Jutten Juraj Kopacek Sylvia Pastorekova Albert J. van der Kogel Philippe Lambin Willem Voncken Kasper M. A. Rouschop Bradly G. Wouters 《The Journal of biological chemistry》2009,284(36):24204-24212
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Juana Maria Lugo Aymé Oliva Antonio Morales Osvaldo Reyes Hilda Elisa Garay Fidel Herrera Ania Cabrales Ever Pérez Mario Pablo Estrada 《Journal of peptide science》2010,16(11):633-643
To date, many technologies have been developed to increase efficiency in aquaculture, but very few successful biotechnology molecules have arrived on the market. In this context, marine biotechnology has an opportunity to develop products to improve the output of fish in aquaculture. Published in vivo studies on the action of the pituitary adenylate cyclase‐activating polypeptide (PACAP) in fish are scarce. Recently, our group, for the first time, demonstrated the biological role of this neuropeptide administrated by immersion baths in the growth and development of larval fish. In this work, we have evaluated the effects of recombinant Clarias gariepinus PACAP administration by intraperitoneal injection on growth performance and feeding behavior in juvenile fish. Our results showed the physiological role of this peptide for growth control in fish, including the juvenile stage, and confirm that its biological functions are well conserved in fish, since C. gariepinus PACAP stimulated growth in juvenile tilapia Oreochromis niloticus. In addition, we have observed that the growth‐promoting effect of PACAP in juvenile tilapia was correlated with higher GH concentration in serum. With regard to the neuroendocrine regulation of growth control by PACAP, it was demonstrated that PACAP stimulates food intake in juvenile tilapia. In general, PACAP appears to act in the regulation of the growth control in juvenile fish. These findings propose that PACAP is a prominent target with the potential to stimulate fish growth in aquaculture. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
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Gabriela C. Olivera Maria C. Albareda Maria G. Alvarez Ana M. De Rissio Laura E. Fichera Gretchen Cooley Pedro Yachelini Hugo A. Hrellac Hilda Riboldi Susana A. Laucella Rick L. Tarleton Miriam Postan 《Microbes and infection / Institut Pasteur》2010,12(5):359-363
Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-γ ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-γ responses were detected in 42% of subjects evaluated (15/26 positive by conventional serology and 22/62 seronegative subjects). Using immunoblotting, T. cruzi-specific IgG reactivity was detected in all seropositive subjects and in 11% (7/61) of subjects negative by conventional serology. Measurements of T cell responses and antibodies by immunoblotting, in conjunction with conventional serology, might enhance the capability of detection of exposure to T. cruzi in endemic areas. 相似文献