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61.
Al-Obeidat Feras Bani-Hani Anoud Adedugbe Oluwasegun Majdalawieh Munir Benkhelifa Elhadj 《Cluster computing》2021,24(3):2341-2353
Cluster Computing - Social data analysis has become a vital tool for businesses and organisations for mining data from social media and analysing for diverse purposes such as customer opinion... 相似文献
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63.
Tamara S. Al-Qudah Rida A. Shibli Feras Q. Alali 《In vitro cellular & developmental biology. Plant》2011,47(4):496-505
A protocol for in vitro propagation of the wild germander (Teucrium polium L.) was developed. In vitro plants were developed from ex vitro axillary buds. Then, shoot tips were excised and established on Murashige and Skoog medium. Proliferation of shoots was tested
with different levels of 6-furfurylaminopurin, 6-benzyladenine, or thiadiazuron. The highest proliferation of T. polium was obtained when 6-benzyladenine and 6-furfurylaminopurin were used at 2.0 and 1.6 mg l−1, respectively. Thiadiazuron gave the lowest response for shoot proliferation. Rooting was experimented at different levels
of Indol-3-butric acid, Indol-3-acetic acid, or 1-naphthaleneacetic acid. 1-Naphthaleneacetic was the only growth regulator
which promoted root induction. Rooted plants were acclimatized successfully with 75% survival and grown in the greenhouse.
In vitro- and in vivo-grown plants were analyzed for essential oil production. In vitro-grown T. polium on MS medium supplemented with 6-benzyladenine and 1-naphthaleneacetic gave higher oil yield than that grown on hormone-free
Murashige and Skoog medium. In vivo (wild)-grown T. polium produced different oil yield when collected in different months (April and October). β-caryophyllene, used as a marker compound
in the essential oil, was identified and quantified by gas chromatography (GC) analysis. Gas chromatography/mass (GC-MS) spectrometry
analysis was also used to identify other components of in vitro cultures and to compare with in vivo-grown plants. 相似文献
64.
Andre Bazzone Wagner Steuer Costa Markus Braner Octavian C?linescu Lina Hatahet Klaus Fendler 《Journal of visualized experiments : JoVE》2013,(75)
The electrophysiological method we present is based on a solid supported membrane (SSM) composed of an octadecanethiol layer chemisorbed on a gold coated sensor chip and a phosphatidylcholine monolayer on top. This assembly is mounted into a cuvette system containing the reference electrode, a chlorinated silver wire.After adsorption of membrane fragments or proteoliposomes containing the membrane protein of interest, a fast solution exchange is used to induce the transport activity of the membrane protein. In the single solution exchange protocol two solutions, one non-activating and one activating solution, are needed. The flow is controlled by pressurized air and a valve and tubing system within a faraday cage.The kinetics of the electrogenic transport activity is obtained via capacitive coupling between the SSM and the proteoliposomes or membrane fragments. The method, therefore, yields only transient currents. The peak current represents the stationary transport activity. The time dependent transporter currents can be reconstructed by circuit analysis.This method is especially suited for prokaryotic transporters or eukaryotic transporters from intracellular membranes, which cannot be investigated by patch clamp or voltage clamp methods. 相似文献