全文获取类型
收费全文 | 2044篇 |
免费 | 156篇 |
国内免费 | 205篇 |
专业分类
2405篇 |
出版年
2024年 | 4篇 |
2023年 | 25篇 |
2022年 | 83篇 |
2021年 | 137篇 |
2020年 | 86篇 |
2019年 | 113篇 |
2018年 | 111篇 |
2017年 | 67篇 |
2016年 | 94篇 |
2015年 | 109篇 |
2014年 | 156篇 |
2013年 | 164篇 |
2012年 | 175篇 |
2011年 | 168篇 |
2010年 | 95篇 |
2009年 | 90篇 |
2008年 | 114篇 |
2007年 | 79篇 |
2006年 | 59篇 |
2005年 | 59篇 |
2004年 | 48篇 |
2003年 | 53篇 |
2002年 | 42篇 |
2001年 | 22篇 |
2000年 | 19篇 |
1999年 | 36篇 |
1998年 | 18篇 |
1997年 | 23篇 |
1996年 | 21篇 |
1995年 | 14篇 |
1994年 | 18篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 21篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 6篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1973年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有2405条查询结果,搜索用时 0 毫秒
981.
982.
983.
984.
Peng Li Yongyun Li Liqi Zhu Zhi Yang Jie He Lihua Wang Qingfeng Shang Hui Pan Huixue Wang Xiong Ma Bin Li Xianqun Fan Shengfang Ge Renbing Jia He Zhang 《生物化学与生物物理学报:疾病的分子基础》2018,1864(3):709-720
Liver fibrosis is overly exuberant wound healing that leads to portal hypertension or liver cirrhosis. Recent studies have demonstrated the functions of bone morphogenetic protein 9 (BMP9) in liver fibrosis, and thus, targeting liver-specific BMP9 abnormalities will become an attractive approach for developing therapeutics to treat liver fibrosis. Here, we reveal that BMP9 serves as a valuable serum diagnostic indicator and efficient therapeutic target to attenuate liver fibrogenesis. Our analysis of biopsies from liver fibrotic patients revealed that higher BMP9 levels accompanied advanced stages of liver fibrosis. In mouse models, recombinant Bmp9 overexpression accelerated liver fibrosis, and adenovirus-mediated Bmp9 knockdown attenuated liver fibrogenesis. Intriguingly, BMP9 directly stimulated hepatic stellate cell activation via the SMAD signaling pathway to enhance hepatic fibrosis. Moreover, an inhibitory monoclonal antibody targeting Bmp9 was efficacious in treatment of mice with liver fibrosis. These observations delineate a novel model in which BMP9 directly drives SMAD/ID1 signaling in hepatic stellate cells, which modulates liver fibrogenesis development. Moreover, the findings unveil a promising surrogate biomarker for the diagnosis of hepatic fibrosis, thereby representing an efficient “BMP9 neutralization” approach in alleviating hepatic fibrosis. 相似文献
985.
Junjie Zhang Yimin Shang Entao Wang Wenfeng Chen Philippe de Lajudie Benyin Li Chen Guo Xu Yang Jianqiang Zheng Chunzeng Liu 《Plant and Soil》2018,425(1-2):201-215
Background and aims
Common bean (Phaseolus vulgaris L.) nodulates with a wide range of rhizobia. Amongst these is Bradyrhizobium, which is inefficient but able to induce profuse nodulation on this crop. Based on this observation, we tested whether co-inoculating bradyrhizobia with a more standard common bean symbiont, Rhizobium tropici, could stimulate growth and nodulation of common bean, thus contributing to a more effective symbiosis.Methods
Rhizobium tropici was co-inoculated with two Bradyrhizobium strains applied at three different doses (104, 106, and 108 CFU seed?1) under sterile conditions, and at a single dose (108 CFU seed?1) in non-sterile soil. Plant biomass, nodulation, and N accumulation in plant tissues were evaluated.Results
Co-inoculated plants produced more nodules, and accumulated more shoot dry biomass and nitrogen than plants inoculated with R. tropici alone under gnotobiotic conditions. Significant responses were observed at the highest inoculum dose and a significant correlation between dose and shoot dry weight was observed. Co-inoculation increased biomass and N accumulation in non-sterile soil, although with a smaller magnitude.Conclusions
Altogether, our findings suggest that the co-inoculation with bradyrhizobia contributed to an improved symbiotic interaction between R. tropici and common beans.986.
987.
988.
989.
Xiuling Shang Xin Chai Xuemei Lu Yuan Li Yun Zhang Guoqiang Wang Chen Zhang Shuwen Liu Yu Zhang Jiyin Ma Tingyi Wen 《Biotechnology letters》2018,40(2):383-391
Objective
To identify useful native promoters of Corynebacterium glutamicum for fine-tuning of gene expression in metabolic engineering.Results
Sixteen native promoters of C. glutamicum were characterized. These promoters covered a strength range of 31-fold with small increments and exhibited relatively stable activity during the whole growth phase using β-galactosidase as the reporter. The mRNA level and enzymatic activity of the lacZ reporter gene exhibited high correlation (R 2 = 0.96) under the control of these promoters. Sequence analysis found that strong promoters had high similarity of the -10 hexamer to the consensus sequence and preference of the AT-rich UP element upstream the -35 region. To test the utility of the promoter library, the characterized native promoters were applied to modulate the sucCD-encoded succinyl-CoA synthetase expression for l-lysine overproduction.Conclusions
The native promoters with various strengths realize the efficient and precise regulation of gene expression in metabolic engineering of C. glutamicum.990.