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我国巴马小型猪SLA-2基因克隆及分子特征 总被引:1,自引:0,他引:1
为研究我国巴马小型猪SLA-Ⅰ分子特征,设计引物克隆了SLA-Ⅰ类分子SLA-2基因(SLA-2*bm),并通过分子生物学软件分析其分子特征。经克隆及序列测定分析,SLA-2*bm为1119bp,其中3~1097为ORF区,共编码364个氨基酸,分别在第125、188、227和283位置出现半胱氨酸残基,含有两对链内二硫键。氨基酸同源性分析显示SLA-2*bm与其它SLA-2、 SLA-3和SLA-1序列的同源率分别为88.4%~96.4%、88.3%~90.5%和87.7%~92.7%。系统进化树显示SLA-2*bm与其它SLA-2等位基因在遗传关系上相对独立,进化程度较低;各功能区分析,SLA-2*bm与人HLA-A2和小鼠H-2K分子结构相似,且保留了人HLA-A2基因的部分功能位点。结果表明,SLA-2*bm属于一个新的等位基因,巴马小型猪是保留原始基因特征的品种。 相似文献
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大量遗传性疾病的发生是由于基因突变引起蛋白质错误折叠而不能运输到作用位点,从而导致功能缺陷.近年来兴起的药物分子伴侣是恢复蛋白质折叠运输缺陷的新疗法,这类化合物一般为目的蛋白的底物类似物、受体配基或酶抑制剂等化学小分子,具细胞通透性,能在内质网中特异性识别并结合突变蛋白,校正并稳定其正确构象,协助其运输到正确位点,直接恢复突变蛋白功能,可治疗各种南蛋白质折叠运输缺陷导致的内分泌及代谢疾病.目前已报道的由药物分子伴侣恢复功能的突变蛋白主要为质膜蛋白及细胞器蛋白,如ATP结合盒转运蛋白、G-蛋白耦联受体及溶酶体酶等.大量的细胞及动物实验结果显示了药物分子伴侣的临床应用前景广阔,目前已有一例临床实验获得了成功. 相似文献
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It has been predicted that nonameric peptides I (VP126–34, RRQHTDVSF), II (VP1157–165, RTLPTSFNY) and III (VP145–53, KEQVNVLDL) from the VP1 capsid protein of the foot-and-mouth disease virus (FMDV) are T cell epitopes. To investigate whether these peptides have immunological activity, BALB/c mice were immunized with peptide I, II or III conjugated with immunostimulating complexes (ISCOMs). A cytotoxic T lymphocyte assay was used to evaluate the cytotoxic activity induced by peptides along with by measuring peptide-specific T-cell proliferation and CD8+ T lymphocyte numbers in whole blood and interferon (IFN)-γ production in peripheral blood mononuclear cells induced by peptides. To further identify the protective efficacy of peptides, an FMDV challenge assay was done in guinea pigs. Peptides I and II stimulated significant increases in T-cell proliferation, CD8+ T lymphocytes, and IFN-γ secretion and cytotoxic activity compared to controls. The FMDV challenge assay indicated peptides I and II can protect over 60% of animals from virus attack. The results demonstrate that peptides I and II encapsulated in liposomes should be CTL epitopes of FMDV and can protect animals from virus attack to some extent. 相似文献
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Jing-Shu Zhang Da-Ming Li Yue Ma Ning He Qing Gu Feng-Shan Wang Shu-Qing Jiang Bing-Qing Chen Jia-Ren Liu 《PloS one》2013,8(2)
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer. 相似文献
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中国近海文蛤属(双壳纲,帘蛤科)的系统分类学研究 总被引:5,自引:0,他引:5
长期以来,我国的文蛤分类存在着严重的问题和混乱现象,它已影响和制约了我国文蛤的水产养殖和遗传育种等相关研究。利用比较形态学和分子生物学技术,对我国沿海分布的文蛤属进行了系统的分类学研究,澄清了混淆种,修订了物种名称,确立了我国沿海文蛤的分类地位,对文蛤属的分类系统进行分子重建。结果表明短文蛤和丽文蛤为近缘种先聚到一起,然后与斧文蛤聚到一起,而琴文蛤、文蛤与该属其它 3 种文蛤的分歧时间较早。研究结果将为文蛤的分类研究、遗传育种、资源保护和利用提供科学依据。 相似文献
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Zhu Hao-Miao Sun Bin Li Yi-Jun Meng Dan-Hua Zheng Shuang Wang Ting-Ting Wang Feng-Shan Sheng Ju-Zheng 《Applied microbiology and biotechnology》2018,102(2):751-761
Applied Microbiology and Biotechnology - Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This... 相似文献
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为研究我国巴马小型猪SLA-Ⅰ分子特征,设计引物克隆了SLA-Ⅰ类分子SLA-2基因(SLA-2*bm),并通过分子生物学软件分析其分子特征。经克隆及序列测定分析,SLA-2*bm为1119bp,其中3~1097为ORF区,共编码364个氨基酸,分别在第125、188、227和283位置出现半胱氨酸残基,含有两对链内二硫键。氨基酸同源性分析显示SLA-2*bm与其它SLA-2、 SLA-3和SLA-1序列的同源率分别为88.4%~96.4%、88.3%~90.5%和87.7%~92.7%。系统进化树显示SLA-2*bm与其它SLA-2等位基因在遗传关系上相对独立,进化程度较低;各功能区分析,SLA-2*bm与人HLA-A2和小鼠H-2K分子结构相似,且保留了人HLA-A2基因的部分功能位点。结果表明,SLA-2*bm属于一个新的等位基因,巴马小型猪是保留原始基因特征的品种。 相似文献