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941.
To study intraspecific differences in N utilization in response to enhanced UV-B radiation, field experiments were conducted on two Erigeron breviscapus populations (Huguo and Cangshan), which were respectively obtained from low altitude (UV-B sensitive) and high altitude (UBV-B resistant).The effects of soil nitrogen (N) application (0, 15, 30, 45 g m2) on free amino acid content, the activities of nitrate reductase (NR) and glutamine synthetase (GS), total nitrogen content and N mass in leaves were determined under enhanced UV-B radiation (5 kJ m2) for both populations. The results showed that under enhanced UV-B radiation: (1) increases in total N contents in leaves of the Huguo and Cangshan populations correlated with the amount of N applied. Additionally, leaf biomass of Huguo treated with 15 g m?2 N application and Cangshan with 30 g m?2 N application were higher than that of other treatments. Leaf N masses were highest in both E. breviscapus populations treated with 30 g m?2 N; (2) increases in contents of free amino acids in leaves of both E. breviscapus populations correlated with the amount of applied nitrogen; (3) increases of NR activity in leaves correlated with the amount of applied nitrogen; (4) GS activity in leaves of the Huguo and Cangshan E. breviscapus populations were highest with respective N applications of 15 g m?2 N and 30 g m?2 N. In general, under enhanced UV-B radiation, N application might affect NR and GS and change free amino acid content, resulting in changes in total nitrogen content, biomass and N mass. The optimal amount of supplemental N for N accumulation in E. breviscapus was 30 g m?2 N under enhanced UV-B radiation. 相似文献
942.
Rubia austrozhejiangensis Z. P. Lei, Y. Y. Zhou & R. W. Wang, a new species of Rubiaceae from China, is described and illustrated. The new species is similar to R. ovatifolia Z. Ying Zhang and R. argyi (H. Lév. & Vaniot) H. Hara ex Lauener, but differs from the former in having stems and branches cylindrical, not quadrate‐angled, long‐ovate to ovate‐lanceolate leaf blades, many‐flowered inflorescence, and smaller mericarps, 3–4 mm in diameter. In R. ovatifolia, stems and branches are quadrate‐angled, leaf blades ovate, ovate‐cordate to rounded cordate, and the inflorescences are sparsely flowered. Compared to R. argyi, the new species has cylindrical, not quadrate‐angled stems and branches, leaf blades that are long‐ovate to ovate‐lanceolate, 3–5‐veined, and slightly reflexed corolla lobes. In R. argyi, stems and branches are quadrate‐angled or winged, the corolla lobes are spreading, and the mericarps are 5–7 mm in diameter. 相似文献
943.
944.
Sarah M. Strycharz-Glaven Richard H. Glaven Zheng Wang Jing Zhou Gary J. Vora Leonard M. Tender 《Applied and environmental microbiology》2013,79(13):3933-3942
Microbial solar cells (MSCs) are microbial fuel cells (MFCs) that generate their own oxidant and/or fuel through photosynthetic reactions. Here, we present electrochemical analyses and biofilm 16S rRNA gene profiling of biocathodes of sediment/seawater-based MSCs inoculated from the biocathode of a previously described sediment/seawater-based MSC. Electrochemical analyses indicate that for these second-generation MSC biocathodes, catalytic activity diminishes over time if illumination is provided during growth, whereas it remains relatively stable if growth occurs in the dark. For both illuminated and dark MSC biocathodes, cyclic voltammetry reveals a catalytic-current–potential dependency consistent with heterogeneous electron transfer mediated by an insoluble microbial redox cofactor, which was conserved following enrichment of the dark MSC biocathode using a three-electrode configuration. 16S rRNA gene profiling showed Gammaproteobacteria, most closely related to Marinobacter spp., predominated in the enriched biocathode. The enriched biocathode biofilm is easily cultured on graphite cathodes, forms a multimicrobe-thick biofilm (up to 8.2 μm), and does not lose catalytic activity after exchanges of the reactor medium. Moreover, the consortium can be grown on cathodes with only inorganic carbon provided as the carbon source, which may be exploited for proposed bioelectrochemical systems for electrosynthesis of organic carbon from carbon dioxide. These results support a scheme where two distinct communities of organisms develop within MSC biocathodes: one that is photosynthetically active and one that catalyzes reduction of O2 by the cathode, where the former partially inhibits the latter. The relationship between the two communities must be further explored to fully realize the potential for MSC applications. 相似文献
945.
Feng Hou 《Ethnic and racial studies》2013,36(9):1591-1609
While black–white intermarriage is uncommon in the USA, blacks in Canada are just as likely to marry whites as to marry blacks. Asians, in contrast, are more likely to marry whites in the USA than in Canada. We test the claim that high rates of interracial marriage are indicative of high levels of social integration against Peter Blau's ‘macrostructural’ thesis that relative group size is the key to explaining differences in intermarriage rates across marriage markets. Using micro-data drawn from the American Community Survey and the Canadian census, we demonstrate that the relative size of racial groups accounts for over two-thirds of the USA–Canada difference in black–white unions and largely explains the cross-country difference in Asian–white unions. Under broadly similar social and economic conditions, a large enough difference in relative group size can become the predominant determinant of group differences in the prevalence of interracial unions. 相似文献
946.
Xiao K. Wang Qiu F. Ma Wei Jiang Jing Lv Wei D. Pan Tao Song 《Geomicrobiology journal》2013,30(6):296-303
Magnetotactic bacteria synthesize intracellular magnetic particles, magnetosomes, which arrange in chain(s) and confer on cell a magnetic dipolar moment. To explore the function of geomagnetic field to magnetotactic bacteria, the effects of hypomagnetic field on magnetosome formation in Magnetospirillum magneticum AMB-1 were studied. Cells were cultivated in a specially designed device where geomagnetic field was reduced by about 100-fold to less than 500nT. AMB-1 cultures were incubated in hypomagnetic field or geomagnetic field. Results showed that hypomagnetic field had no significant effects on the average number of magnetic particles per bacterium and bacterial iron depletion. However, the growth (OD) of cell at stationary-phase was lower and cellular magnetism (R mag) at exponential growth phase was higher than that of bacteria cultivated in geomagnetic field. Statistic results on transmission electron microscopy (TEM) micrographs showed that the average size of magnetic particles in AMB-1 cells in hypomagnetic field group was larger than that of in geomagnetic field group and more ratio of larger-size magnetic particles (>50 nm) was observed when cultivated 16 h under hypomagnetic field. Furthermore, the influences of hypomagnetic field on gene expression were studied in AMB-1 cells. Quantitative RT-PCR results showed that hypomagnetic field up-regulated mms13, down-regulated mms6 and had no effect on magA. Together, the results showed that hypomagnetic field could affect the growth of AMB-1 at the stationary-phase, the crystallization process of magnetosomes, and mms13, mms6 expressions. In addition, our results suggested that the geomagnetic field plays an important role in the biomineralization of magnetosomes. 相似文献
947.
Jing Wang Hairong Li Yonghua Li Jiangping Yu Linsheng Yang Fujian Feng Zhuo Chen 《Biological trace element research》2013,156(1-3):367-375
To clarify the relationship between the soil selenium distribution and its bioavailability with the distribution of Kashin–Beck disease (KBD) endemic areas on the eastern edge of the Tibetan Plateau, samples of natural soil (0–20 cm), cultivated topsoil, and main crops of the region (highland barley) were collected at different altitudes according to topographical and geomorphological features in both KBD and non-KBD areas of Songpan County. These samples were used for determination and analysis of total selenium content in soil and highland barley and available selenium that can be absorbed and utilized by plants. The results showed that the average total selenium content of natural and cultivated topsoil in KBD areas was lower than that in non-KBD areas (natural soil, P?=?0.061; cultivated soil, P?=?0.002), which is in agreement with the geographical distributions of selenium in other KBD-affected areas. However, the total soil selenium content exhibits certain micro-spatial distribution features, namely, the total selenium content in some endemic areas was significantly higher than that of non-KBD areas. This result was contrary to the general distribution that total selenium content in a KBD-affected area is lower than that in a non-KBD area. We further studied the extraction rate and content of soil selenium in six different fractions. The results indicated that the content and extraction rate of available selenium in KBD-affected areas were significantly lower than those in non-KBD areas. There is a distinct positive correlation between plant-available selenium and highland barley selenium (r?=?0.875, P?=?0.001) and a distinct negative correlation with altitude (r?=??0.801, P?=?0.010). Therefore, in KBD endemic areas, the selenium content in crops decreases as the available selenium content in soil decreases and is closely related to the geographical environment features (such as altitude and precipitation). These results suggest that the soil available selenium and ecological features are important factors that restrict the dietary selenium flux for residents in KBD endemic areas of the Tibetan Plateau, providing a theoretical and experimental basis for implementing agricultural measures to regulate the ecological cycle of the selenium flux in the KBD endemic area. 相似文献
948.
Q. Li X. Z. Li T. Wang L. W. Zhou H. Q. Feng L. Gao J. R. Pei C. Lin C. X. Jiang 《Biological trace element research》2013,151(1):14-17
The aim of the present study was to explore the role of selenoprotein P (SePP) in the etiology of the endemic sudden cardiac death in Yunnan, China. The levels of SePP of 124 subjects and glutathione peroxidase (GPx) of 119 subjects were measured. The subjects were from the old and new endemic areas and non-endemic areas. The levels of SePP and GPx of the subjects of the old endemic area were significantly higher than those of the subjects of the new endemic area and the non-endemic areas, respectively. The Pearson’s correlation among SePP, GPx, and the number of the incident cases of the disease were statistically significant. These correlations show that there is an inverse relationship among the number of patients and the levels of SePP (r? = ?? 0.9800, P ?= ?0.0200) and GPx (r? = ?? 0.961, P ?= ?0.009). The results show that selenium deficiency might play an important role in the incidence of the disease. 相似文献
949.
Wei Zhang Hongqi Feng Yanhui Gao Liyan Sun Jing Wang Yuanyuan Li Cheng Wang Lijun Zhao Xinxin Hu Huixin Sun Yudan Wei Dianjun Sun 《Biological trace element research》2013,151(2):269-276
Although studies have shown that arsenic exposure can induce apoptosis in a variety of cells, the exact molecular mechanism of chronic arsenicosis remains unclear. Based on our previous study on human serum, the present study was to determine whether pigment epithelium-derived factor (PEDF) plays a role in the damage induced by chronic arsenic exposure in a rat model and to explore the possible signaling pathway involved. Thirty male Wistar rats were randomly divided into three groups and the arsenite doses administered were 0, 10, and 50 mg/L, respectively. The experiment lasted for 6 months. Our results showed that level of arsenic increased significantly in serum, liver, brain, and kidney in arsenic-exposed groups. It was indicated that PEDF protein was widely distributed in the cytoplasm of various types of cells in liver, brain, and kidney. PEDF protein level was only changed when the arsenite dose reached 50 mg/L in liver and brain, whereas it was not changed in the kidney. In order to investigate the possible mechanism of PEDF-exerted damages upon arsenite exposure, apoptosis in liver and brain was assessed. The proportion of apoptotic cells gradually increased with increasing arsenic administration. The ratio of Bax/Bcl-2 in the high arsenic group (50 mg/L) was significantly higher than that in the control group. Therefore, we thought PEDF played a role in cell apoptosis of liver and brain which induced by sodium arsenite exposure, and the results also demonstrated that Bax and Bcl-2 might be two key targets in the action of PEDF. 相似文献
950.
Jing Zhang Yang Zhou Lin Ma Shunquan Huang Ruijin Wang Rongrong Gao Youjun Wu Hongjun Shi Jun Zhang 《Biological trace element research》2013,152(2):267-274
Nickel is an important kind of metal and a necessary trace element in people’s production and livelihood; it is also a well-confirmed human carcinogen. In the past few years, researchers did a large number of studies about the molecular mechanisms of nickel carcinogenesis, and they focused on activation of proto-oncogenes and inactivation of anti-oncogenes caused by gene point mutation, gene deletion, gene amplification, DNA methylation, chromosome condensation, and so on that were induced by nickel. However, the researches on tumorigenic molecular mechanisms regulated by microRNAs (miRNAs) are rare. In this study, we established nickel-induced tumor by injecting Ni3S2 compounds to Wistar Rattus. By establishing a cDNA library of miRNA from rat muscle tumor tissue induced by Ni3S2, we found that the expression of miR-222 was significantly upregulated in tumor tissue compared with the normal tissue. As we expected, the expression levels of target genes of miR-222, CDKN1B and CDKN1C, were downregulated in the nickel-induced tumor. The same alteration of miR-222 and its target genes was also found in malignant 16HBE cells induced with Ni3S2 compounds. We conclude that miR-222 may promote cell proliferation infinitely during nickel-induced tumorigenesis in part by regulating the expression of its target genes CDKN1B and CDKN1C. Our study elucidated a novel molecular mechanism of nickel-induced tumorigenesis. 相似文献