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21.
Ioanna Ntai Richard D. LeDuc Ryan T. Fellers Petra Erdmann-Gilmore Sherri R. Davies Jeanne Rumsey Bryan P. Early Paul M. Thomas Shunqiang Li Philip D. Compton Matthew J. C. Ellis Kelly V. Ruggles David Feny? Emily S. Boja Henry Rodriguez R. Reid Townsend Neil L. Kelleher 《Molecular & cellular proteomics : MCP》2016,15(1):45-56
22.
Rebecca Torisky John P. Fellers Glenn B. Collins 《Plant Molecular Biology Reporter》1996,14(2):124-133
The DuPont/BioRad PDS1000/He has become an important tool in genetic transformation. With the present design, however, it
is difficult to accurately predict the impact area. This report discusses targeting devices that were designed to accurately
focus biolistic particles in a given area. Tobacco leaves and soybean tissue were used to evaluate the efficiency of the device.
With the focusing device, GUS positive foci were confined in a defined ring on soybean cotyledonary nodes. Approximately 65
percent of the GUS positive foci on tobacco leaves were concentrated in a ring 8 to 16 mm from the center of the short, compared
to 27 percent without the device. The results demonstrate that PDS-1000 biolistic particles can both be accurately and predictably
delivered at target tissue. 相似文献
23.
BAC-FISH in wheat identifies chromosome landmarks consisting of different types of transposable elements 总被引:12,自引:0,他引:12
Fluorescence in situ hybridization (FISH) has been widely used in the physical mapping of genes and chromosome landmarks in plants and animals. Bacterial artificial chromosomes (BACs) contain large inserts making them amenable for FISH mapping. We used BAC-FISH to study genome organization and evolution in hexaploid wheat and its relatives. We selected 56 restriction fragment length polymorphism (RFLP) locus-specific BAC clones from libraries of Aegilops tauschii (the D-genome donor of hexaploid wheat) and A-genome diploid Triticum monococcum. Different types of repetitive sequences were identified using BAC-FISH. Two BAC clones gave FISH patterns similar to the repetitive DNA family pSc119; one BAC clone gave a FISH pattern similar to the repetitive DNA family pAs1. In addition, we identified several novel classes of repetitive sequences: one BAC clone hybridized to the centromeric regions of wheat and other cereal species, except rice; one BAC clone hybridized to all subtelomeric chromosome regions in wheat, rye, barley and oat; one BAC clone contained a localized tandem repeat and hybridized to five D-genome chromosome pairs in wheat; and four BAC clones hybridized only to a proximal region in the long arm of chromosome 4A of hexaploid wheat. These repeats are valuable markers for defined chromosome regions and can also be used for chromosome identification. Sequencing results revealed that all these repeats are transposable elements (TEs), indicating the important role of TEs, especially retrotransposons, in genome evolution of wheat.Communicated by P.B. Moens 相似文献
24.
25.
Development and Characterization of a Nonmorphogenetic Cell Line of Wheat (Triticum aestivum) 总被引:1,自引:1,他引:0
Arron C. Guenzi Kay Scheets J. Larry Green John P. Fellers 《Plant Cell, Tissue and Organ Culture》2004,78(1):23-28
This study was conducted to compare characteristics of a wheat (Triticum aestivum L.) cell line to those of the maize (Zea mays L.) black Mexican sweet (BMS) cell line and to compare protoplasts isolated from suspension cells of these cell lines. The
wheat cell line was established from immature-embryo derived callus of the experimental line ‘ND7532’ and was conditioned
for growth in suspension culture. For both cell lines, measurements of packed cell volume (PCV), fresh weight (FW), and dry
weight (DW) were taken at 3 day intervals from suspension cultures. Measurements of FW of calluses cultured from suspension
cells of both cell lines were taken at 6 day intervals. The morphogenetic potential of the wheat ND7532 cell line was tested
in both callus and suspension cultures using media promoting regeneration and/or organogenesis. Growth rates of ND7532 cells
in suspension culture were comparable to those of BMS cells. However, relative growth rates of calluses recovered from ND7532
suspension cells were slower than those of calluses recovered from BMS suspension cells. The ND7532 cell line has very limited
morphogenetic potential and has been maintained as rapidly growing callus tissue for 11 years. Yields of protoplasts from
suspension cells of the two cell lines were comparable, though ND7532 protoplasts were typically smaller. The wheat cell line
has is now designated ND7532-NM (nonmorphogenetic) and is available for cellular and molecular biology research. 相似文献
26.
Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat 总被引:20,自引:0,他引:20
We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a gene-rich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat. 相似文献
27.
Leah V. Schaffer Robert J. Millikin Rachel M. Miller Lissa C. Anderson Ryan T. Fellers Ying Ge Neil L. Kelleher Richard D. LeDuc Xiaowen Liu Samuel H. Payne Liangliang Sun Paul M. Thomas Trisha Tucholski Zhe Wang Si Wu Zhijie Wu Dahang Yu Michael R. Shortreed Lloyd M. Smith 《Proteomics》2019,19(10)
A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post‐translational modifications. In top‐down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top‐down proteomic workflows. In this review, some recent advances are outlined and current challenges and future directions for the field are discussed. 相似文献
28.
29.
Fellers CR 《Journal of bacteriology》1926,12(3):181-202
30.
Li W Zhang P Fellers JP Friebe B Gill BS 《The Plant journal : for cell and molecular biology》2004,40(4):500-511
We investigated the composition and the basis of genome expansion in the core Triticeae genome using Aegilops tauschii, the D-genome donor of bread wheat. We sequenced an unfiltered genomic shotgun (trs) and a methylation-filtration (tmf) library of A. tauschii, and analyzed wheat expressed sequence tags (ESTs) to estimate the expression of genes and transposable elements (TEs). The sampled D-genome sequences consisted of 91.6% repetitive elements, 2.5% known genes, and 5.9% low-copy sequences of unknown function. TEs constituted 68.2% of the D-genome compared with 50% in maize and 14% in rice. The DNA transposons constituted 13% of the D-genome compared with 2% in maize. TEs were methylated unevenly within and among elements and families, and most were transcribed which contributed to genome expansion in the core Triticeae genome. The copy number of a majority of repeat families increased gradually following polyploidization. Certain TE families occupied discrete chromosome territories. Nested insertions and illegitimate recombination occurred extensively between the TE families, and a majority of the TEs contained internal deletions. The GC content varied significantly among the three sequence sets examined ranging from 42% in tmf to 46% in trs and 52% in the EST. Based on enrichment of genic sequences, methylation-filtration offers one option, although not as efficient as in maize, for isolating gene-rich regions from the large genome of wheat. 相似文献