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991.
Martins FT Doriguetto AC de Souza TC de Souza KR Dos Santos MH Moreira ME Barbosa LC 《化学与生物多样性》2008,5(2):251-258
The composition of the volatile oil obtained by hydrodistillation from the fruit peel of Garcinia brasiliensis (Mart.) Planch. et Triana was determined by GC/MS. A total of 38 components were identified, including gamma-muurolene (10.3%), spathulenol (8.7%), delta-cadinene (8.3%), torreyol (8.0%), alpha-cadinol (7.0%), cadalene (6.3%), and gamma-cadinene (5.3%). Oxygenated sesquiterpenes (43%) were the main group of compounds. The anti-inflammatory activity of the volatile oil was evaluated through the rat-paw edema model induced by carrageenan. Inhibition of the inflammatory process was noticed 3 h after carrageenan administration. In addition, the volatile oil showed poor antioxidant activity. 相似文献
992.
Karin Neukam Nuria Pastor Felipe Corts 《Mutation Research - Genetic Toxicology and Environmental Mutagenesis》2008,654(1):8-12
Tea polyphenols are promising chemopreventive anticancer agents, the properties of which have been studied both in vitro and in vivo, providing evidence that – within this group of compounds – the tea flavanols are able to inhibit carcinogenesis, an effect that in some cases could be correlated with increased cell apoptosis and decreased cell proliferation. Of four main tea flavanols, namely (−)-epigallocatechin-3-gallate (EGCG), (−)-epigallocatechin (EGC), (+)-catechin (CA) and (−)-epicatechin (EC), it was found that EGCG was the most potent to inhibit dose dependently the topoisomerase II (TOPO II) catalytic activity isolated from hamster ovary AA8 cells. In the range of concentrations that caused TOPO II inhibition, a high level of endoreduplication, a rare phenomenon that consists in two successive rounds of DNA replication without intervening mitosis, was observed, while neither micronuclei nor DNA strand breaks (Comet assay) were detected at the same doses. We propose that the anticarcinogenic effect of tea flavanols can be partly explained by their potency and effectiveness to induce endoreduplication. Concerning such an induction, maximum effect seems to require a pyrogallol structure at the B-ring. Additional substitution with a galloylic residue at the C3 hydroxyl group leads to further augmentation of the effect. Thus, we suggest that the chemopreventive properties of tea flavanols can be at least partly due to their ability to interfere with the cell cycle and block cell proliferation at early stages of mitosis. 相似文献
993.
Remy Lian Ware Felipe Ramon-Portugal Alexandra Magro Christine Ducamp Jean-Louis Hemptinne Michael E. N. Majerus 《BioControl》2008,53(1):189-200
Intraspecific and interspecific predation of eggs is a well documented phenomenon amongst aphidophagous coccinellids. The
invasive species Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) is known to be a top intraguild predator and reported to attack the eggs of many coccinellid
species both in a laboratory setting and in the wild. A previous laboratory study highlighted that while many species’ eggs
were highly palatable to H. axyridis, the eggs of Calvia quatuordecimguttata (Linnaeus) (Coleoptera: Coccinellidae) appeared to be extremely well protected from attack. Here we present the results of
behavioural experiments testing the hypothesis that substances on the egg surface are responsible for this protection, and
report preliminary results of GC-MS analysis of these compounds. When the coatings of C. quatuordecimguttata eggs were removed using hexane, they became significantly more susceptible to predation by neonate H. axyridis larvae. However, their overall palatability was not affected, in that complete consumption was never or rarely observed.
This suggests that the surface compounds are a true indicator of unpalatability in this species. The effect of hexane-washing
on already palatable conspecific eggs was also analysed but had no significant effect on the susceptibility of eggs to cannibalism.
We conclude that the eggs of at least one European species are effectively protected by surface deterrents from intraguild
predation by H. axyridis. This effect might be due to both the diversity and abundance of hydrocarbons present within the egg coating, the presence
of alkenes and/or the presence of patches of a red substance on the eggs’ surface, which is thought to belong to the acid
group. In conjunction with data on the susceptibility of other immature stages of C. quatuordecimguttata, this finding may indicate a decreased risk of the species falling victim to invasive H. axyridis, despite their coincident habitat ranges. 相似文献
994.
Glutathione peroxidase family - an evolutionary overview 总被引:1,自引:0,他引:1
Glutathione peroxidases (EC 1.11.1.9 and EC 1.11.1.12) catalyze the reduction of H(2)O(2) or organic hydroperoxides to water or corresponding alcohols using reduced glutathione. Some glutathione peroxidase isozymes have a selenium-dependent glutathione peroxidase activity and present a selenocysteine encoded by the opal TGA codon. In the present study, insights into the evolution of the whole glutathione peroxidase gene family were obtained after a comprehensive phylogenetic analysis using the improved number of glutathione peroxidase sequences recorded in the PeroxiBase database (http://peroxidase.isb-sib.ch/index.php). The identification of a common ancestral origin for the diverse glutathione peroxidase clusters was not possible. The complex relationships and evolutionary rates of this gene family suggest that basal glutathione peroxidase classes, present in all kingdoms, have originated from independent evolutionary events such as gene duplication, gene losses, lateral gene transfer among invertebrates and vertebrates or plants. In addition, the present study also emphasizes the possibility of some members being submitted to strong selective forces that probably dictated functional convergences of taxonomically distant groups. 相似文献
995.
Taube R Zhu Q Xu C Diaz-Griffero F Sui J Kamau E Dwyer M Aird D Marasco WA 《PloS one》2008,3(9):e3181
Background
Isolation of human antibodies using current display technologies can be limited by constraints on protein expression, folding and post-translational modifications. Here we describe a discovery platform that utilizes self-inactivating (SIN) lentiviral vectors for the surface display of high-affinity single-chain variable region (scFv) antibody fragments on human cells and lentivirus particles.Methodology/Principal Findings
Bivalent scFvFc human antibodies were fused in frame with different transmembrane (TM) anchoring moieties to allow efficient high-level expression on human cells and the optimal TM was identified. The addition of an eight amino acid HIV-1 gp41 envelope incorporation motif further increased scFvFc expression on human cells and incorporation into lentiviral particles. Both antibody-displaying human cells and virus particles bound antigen specifically. Sulfation of CDR tyrosine residues, a property recently shown to broaden antibody binding affinity and antigen recognition was also demonstrated. High level scFvFc expression and stable integration was achieved in human cells following transduction with IRES containing bicistronic SIN lentivectors encoding ZsGreen when scFvFc fusion proteins were expressed from the first cassette. Up to 106-fold enrichment of antibody expressing cells was achieved with one round of antigen coupled magnetic bead pre-selection followed by FACS sorting. Finally, the scFvFc displaying human cells could be used directly in functional biological screens with remarkable sensitivity.Conclusions/Significance
This antibody display platform will complement existing technologies by virtue of providing properties unique to lentiviruses and antibody expression in human cells, which, in turn, may aid the discovery of novel therapeutic human mAbs. 相似文献996.
Grosman AH Janssen A de Brito EF Cordeiro EG Colares F Fonseca JO Lima ER Pallini A Sabelis MW 《PloS one》2008,3(6):e2276
Many true parasites and parasitoids modify the behaviour of their host, and these changes are thought to be to the benefit of the parasites. However, field tests of this hypothesis are scarce, and it is often unclear whether the host or the parasite profits from the behavioural changes, or even if parasitism is a cause or consequence of the behaviour. We show that braconid parasitoids (Glyptapanteles sp.) induce their caterpillar host (Thyrinteina leucocerae) to behave as a bodyguard of the parasitoid pupae. After parasitoid larvae exit from the host to pupate, the host stops feeding, remains close to the pupae, knocks off predators with violent head-swings, and dies before reaching adulthood. Unparasitized caterpillars do not show these behaviours. In the field, the presence of bodyguard hosts resulted in a two-fold reduction in mortality of parasitoid pupae. Hence, the behaviour appears to be parasitoid-induced and confers benefits exclusively to the parasitoid. 相似文献
997.
998.
Rocío González-Vázquez Elia Ramírez-Arriaga Enrique Martínez-Hernández Luis Felipe Jiménez-García Lourdes Teresa Agredano-Moreno 《Grana》2017,56(3):174-186
Desmodium diversity has been mainly classified using fruit characters, and because of variability in these and other characters, the taxonomy has sometimes been problematic and thus, there is a need for clarification and taxonomic reassessments. This is the case for the Desmodium procumbens group, where several infraspecific taxa have been named. The phylogenetic relationships among taxa referred to this group is unknown, and the taxonomic status has been recently revised only for some of the taxa. In this study, pollen morphology and ultrastructure of D. neomexicanum, D. procumbens var. procumbens, D. procumbens var. exiguum, D. procumbens var. longipes and D. procumbens var. transversum, belonging to the Mexican Desmodium procumbens group, were examined using light, phase-contrast, scanning electron and transmission electron microscopy, in order to establish palynological characters that might contribute to a better taxonomic resolution. Pollen grains are tricolporate, radiosymmetric, oblate spheroidal to prolate spheroidal shape, with lalongate endoapertures and rugulate exine with foveolae between rugulae. The exine is tectate with a columellate and/or granulate structure. The absence of a fastigium, features of exine sculpture, a colpus membrane and infrastructure mainly dominated by granules differentiate the pollen grains of the varieties exiguum and transversum from the other taxa studied. Pollen traits and sequence data provide evidence for separating D. neomexicanum from species D. procumbens. In general, the result from the pollen analysis is congruent with results obtained from a preliminary molecular analysis of this group. 相似文献
999.
Effects of single and multiple herbivory by host and non‐host caterpillars on the attractiveness of herbivore‐induced volatiles of sugarcane to the generalist parasitoid Cotesia flavipes
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Maria Fernanda G.V. Peñaflor Felipe G. Gonçalves Camila Colepicolo Patricia A. Sanches José Mauricio S. Bento 《Entomologia Experimentalis et Applicata》2017,165(1):83-93
It is well known that parasitoids are attracted to volatiles emitted by host‐damaged plants; however, this tritrophic interaction may change if plants are attacked by more than one herbivore species. The larval parasitoid Cotesia flavipesCameron (Hymenoptera: Braconidae) has been used intensively in Brazil to control the sugarcane borer, Diatraea saccharalisFabricius (Lepidoptera: Pyralidae) in sugarcane crops, where Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), a non‐stemborer lepidopteran, is also a pest. Here, we investigated the ability of C. flavipes to discriminate between an unsuitable host (S. frugiperda) and a suitable host (D. saccharalis) based on herbivore‐induced plant volatiles (HIPVs) emitted by sugarcane, and whether multiple herbivory (D. saccharalis feeding on stalk + S. frugiperda feeding on leaves) in sugarcane affected the attractiveness of HIPVs to C. flavipes. Olfactometer assays indicated that volatiles of host and non‐host‐damaged plants were attractive to C. flavipes. Even though host‐ and non‐host‐damaged plants emitted considerably different volatile blends, neither naïve nor experienced wasps discriminated suitable and unsuitable hosts by means of HIPVs emitted by sugarcane. With regard to multiple herbivory, wasps innately preferred the odor blend emitted by sugarcane upon non‐host + host herbivory over host‐only damaged plants. Multiple herbivory caused a suppression of some volatiles relative to non‐host‐damaged sugarcane that may have resulted from the unaltered levels of jasmonic acid in host‐damaged plants, or from reduced palatability of host‐damaged plants to S. frugiperda. In conclusion, our study showed that C. flavipes responds to a wide range of plant volatile blends, and does not discriminate host from non‐host and non‐stemborer caterpillars based on HIPVs emitted from sugarcane. Moreover, we showed that multiple herbivory by the sugarcane borer and fall armyworm increases the attractiveness of sugarcane plants to the parasitoids. 相似文献
1000.
José Montalva Victor Sepulveda Felipe Vivallo Daniel Paiva Silva 《Journal of Insect Conservation》2017,21(4):657-666
The Eurasian bumble bee Bombus terrestris Linnaeus has been used commercially for pollination of a large number of crop species worldwide. This species has become invasive in several countries where it has escaped into natural environments. This species has become naturalized in many zones of Chile and southern Argentina, and may potentially invade other regions and countries in South America. These naturalized populations of B. terrestris have been associated with rapid population declines of the native bee B. dahlbomii Guérin-Méneville. We report new records of the exotic bee B. terrestris in the Region de Arica y Parinacota in the far north of Chile, which includes portions of the Atacama Desert. We used species distribution models (SDMs) and multivariate analyses to evaluate whether these occurrences represent new escapes from managed colonies or natural dispersal of the species from its southern invaded range. These reports of B. terrestris indicate a northward expansion of this bee. In our analyses, these new areas of occurrences have environmental conditions similar to those observed in the species’ southern invaded range, and our SDMs predict that B. terrestris dispersal through the Atacama is possible, although not likely given the occasional flower blooming in that region of Chile. These new occurrences in northern Chile reflect a potential for future invasion into other regions of South America by B. terrestris. Future surveys in the area should be intensified to evaluate if viable populations of this invasive species may become established. 相似文献