全文获取类型
收费全文 | 2396篇 |
免费 | 175篇 |
出版年
2024年 | 2篇 |
2023年 | 31篇 |
2022年 | 58篇 |
2021年 | 111篇 |
2020年 | 87篇 |
2019年 | 100篇 |
2018年 | 86篇 |
2017年 | 80篇 |
2016年 | 118篇 |
2015年 | 177篇 |
2014年 | 154篇 |
2013年 | 191篇 |
2012年 | 179篇 |
2011年 | 172篇 |
2010年 | 106篇 |
2009年 | 78篇 |
2008年 | 117篇 |
2007年 | 119篇 |
2006年 | 102篇 |
2005年 | 101篇 |
2004年 | 74篇 |
2003年 | 68篇 |
2002年 | 56篇 |
2001年 | 25篇 |
2000年 | 11篇 |
1999年 | 11篇 |
1998年 | 18篇 |
1997年 | 12篇 |
1996年 | 16篇 |
1995年 | 11篇 |
1994年 | 10篇 |
1993年 | 10篇 |
1992年 | 10篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 8篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 9篇 |
1981年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有2571条查询结果,搜索用时 15 毫秒
21.
22.
Longhe Xu Felipe Matsunaga Jin Xi Min Li Jingyuan Ma 《Journal of biomolecular structure & dynamics》2013,31(11):1833-1840
We recently demonstrated that the anionic detergent sodium dodecyl sulfate (SDS) specifically interacts with the anesthetic binding site in horse spleen apoferritin, a soluble protein which models anesthetic binding sites in receptors. This raises the possibility of other detergents similarly interacting with and occluding such sites from anesthetics, thereby preventing the proper identification of novel anesthetic binding sites. n-Dodecyl β-D-maltoside (DDM) is a non-ionic detergent commonly used during protein-anesthetic studies because of its mild and non-denaturing properties. In this study, we demonstrate that SDS and DDM occupy anesthetic binding sites in the model proteins human serum albumin (HSA) and horse spleen apoferritin and thereby inhibit the binding of the general anesthetics propofol and isoflurane. DDM specifically interacts with HSA (Kd?=?40?μM) with a lower affinity than SDS (Kd?=?2?μM). DDM exerts all these effects while not perturbing the native structures of either model protein. Computational calculations corroborated the experimental results by demonstrating that the binding sites for DDM and both anesthetics on the model proteins overlapped. Collectively, our results indicate that DDM and SDS specifically interact with anesthetic binding sites and may thus prevent the identification of novel anesthetic sites. Special precaution should be taken when undertaking and interpreting results from protein-anesthetic investigations utilizing detergents like SDS and DDM. 相似文献
23.
Daniel R. Henríquez Felipe J. Bodaleo Carolina Montenegro-Venegas Christian González-Billault 《PloS one》2012,7(12)
Microtubule-associated protein 1B (MAP1B) is a neuronal protein involved in the stabilization of microtubules both in the axon and somatodendritic compartments. Acute, genetic inactivation of MAP1B leads to delayed axonal outgrowth, most likely due to changes in the post-translational modification of tubulin subunits, which enhances microtubule polymerization. Furthermore, MAP1B deficiency is accompanied by abnormal actin microfilament polymerization and dramatic changes in the activity of small GTPases controlling the actin cytoskeleton. In this work, we showed that MAP1B interacts with a guanine exchange factor, termed Tiam1, which specifically activates Rac1. These proteins co-segregated in neurons, and interact in both heterologous expression systems and primary neurons. We dissected the molecular domains involved in the MAP1B-Tiam1 interaction, and demonstrated that pleckstrin homology (PH) domains in Tiam1 are responsible for MAP1B binding. Interestingly, only the light chain 1 (LC1) of MAP1B was able to interact with Tiam1. Moreover, it was able to increase the activity of the small GTPase, Rac1. These results suggest that the interaction between Tiam1 and MAP1B, is produced by the binding of LC1 with PH domains in Tiam1. The formation of such a complex impacts on the activation levels of Rac1 confirming a novel function of MAP1B related with the control of small GTPases. These results also support the idea of cross-talk between cytoskeleton compartments inside neuronal cells. 相似文献
24.
Monja-Mio Kelly M. Olvera-Casanova Diego Herrera-Herrera Gaston Herrera-Alamillo Miguel Ángel Sánchez-Teyer Felipe L. Robert Manuel L. 《In vitro cellular & developmental biology. Plant》2020,56(5):662-669
In Vitro Cellular & Developmental Biology - Plant - 相似文献
25.
Santos Valdenice F. Costa Maria S. Campina Fábia F. Rodrigues Renato R. Santos Ana L. E. Pereira Felipe M. Batista Karla L. R. Silva Rafael C. Pereira Raquel O. Rocha Bruno A. M. Coutinho Henrique D. M. Teixeira Claudener S. 《Probiotics and antimicrobial proteins》2020,12(1):82-90
Probiotics and Antimicrobial Proteins - The use of natural products together with standard antimicrobial drugs has recently received more attention as a strategy to combat infectious diseases... 相似文献
26.
27.
28.
29.
Olívia Moraes Ruberti Andressa Silva Sousa Laís Rosa Viana Moiss Felipe Pereira Gomes Alessandra Medeiros Maria Cristina Cintra Gomes Marcondes Luciano de Figueiredo Borges Carlos Cesar Crestani Cristiano Mostarda Telma Ftima da Cunha Moraes Rafael Renatino Canevarolo Maria Andreia Delbin Bruno Rodrigues 《Journal of cellular physiology》2021,236(2):1105-1115
This study aimed to evaluate the impact of aerobic training (AT) on autonomic, cardiometabolic, ubiquitin‐proteasome activity, and inflammatory changes evoked by myocardial infarction (MI) in ovariectomized rats. Female Wistar rats were ovariectomized and divided into four groups: sedentary + sham (SS), sedentary + MI (SI), AT + sham surgery (TS), AT + MI (TI). AT was performed on a treadmill for 8 weeks before MI. Infarcted rats previously subjected to AT presented improved physical capacity, increased interleukin‐10, and decreased pro‐inflammatory cytokines. Metabolomic analysis identified and quantified 62 metabolites, 9 were considered significant by the Vip Score. SS, SI, and TS groups presented distinct metabolic profiles; however, TI could not be distinguished from the SS group. MI dramatically increased levels of dimethylamine, and AT prevented this response. Our findings suggest that AT may be useful in preventing the negative changes in functional, inflammatory, and metabolic parameters related to MI in ovariectomized rats. 相似文献
30.
Edgar E. Becerril-García Francesca Pancaldi Ariel A. Cruz-Villacorta Alma R. Rivera-Camacho Carlos A. Aguilar-Cruz Darren A. Whitehead Rogelio González-Armas Marcial Arellano-Martínez Felipe Galván-Magaña 《Journal of fish biology》2021,99(4):1524-1528
The aim of this study is to provide preliminary observations on the microanatomy of Rhincodon typus skin using histology and electron microscopy analyses. Skin biopsies were obtained from a deceased juvenile male shark (548 cm total length) stranded in La Paz, Mexico, during February 2018. The results of this study evidenced the basic structure of the dermal denticles in the epidermis of the trunk of the shark, as well as the composition of the connective tissue in the hypodermis. Histological images of the hypodermis showed a high concentration of collagen fibres, formed by a large number of fine and wavy fibres of compact shape and little intercellular substance. 相似文献