The influence of the synthetic antioxidant fenozan-K on the effects of pentobarbital during dissociative learning

Dependence was studied of pentobarbital effects at dissociated learning in rats of KM line after intraperitoneal injection of antioxidant phenozan-K. It was shown that preliminary (before 1.5 hour) injection of 40 mg/kg of phenozan-K to animals changed the composition (index of oxidation S) of cells lipids of rats brain membranes and simultaneously increased the sensitivity to pentobarbital. Injection of half a dose of pentobarbital, which caused dissociated state, against the background of antioxidant action had the same effect as the full dose (15 mg/kg).     

Study of heterogeneity of the chromatin endonuclease fragment by free flow electrophoresis]

The oligomer chromatin fragments relatively uniform in size (8--11 nucleosomes) were prepared by a short-term endonycleolysis. The heterogeneity of these fragments with respect to their electrophoretic mobility was revealed using free flow electrophoresis. The individual fragmentated chromatin subfractions were obtained. These subfractions differed in their protein and RNA content per DNA weight unit, in quantitative ratios of different zones of high molecular weight non-histone proteins and in thermal and alkaline denaturation kinetics. It was also found that the parameters investigated are correlated with electronegativeity of the fragmentated chromatin subfractions.     

Effects of opioid neuropeptides on the prostaglandin system and lipid peroxidation processes in the myocardium during stress-induced damage

The stress was subsequenced by lowering of Thromboxane A2 level and activation of peroxidation of lipids without any changes of prostacyclin content in myocardium. It was found that the pre-increase of the opioid peptides in blood plasma led to the absence of prostacyclin and thromboxane dynamic and myocardial lipids peroxidation inhibition during the subsequent stress.