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31.
Juventino J. Garcia María L. Hernndez Hugo Torrens Atilano Gutierrez Federico del Río 《Inorganica chimica acta》1995,230(1-2):173-176
By reacting [(C5Me5)M(SRF)2] (forM = Ir, Rf = C6F5 (1a) or C6F4H-p (1b); for M = Rh, Rf = C6F5 (2a) or C6F4H-p (2a)) in toluene with Na[AuCl4], ionic binuclear compounds with the general formula [(C5Me5)M(μ-SRF)2AuCl2]Cl for M = Ir, R = C6F5 (3a) or C6F4H-p (3a); for M = Rh, RF = C6F5 (4a) or C6F4H-p (4b) can be obtained, together with small amounts of [(C5Me5)2Rh2(μ-SRF)(μ-Cl)2]Cl (RF = C6F5 (5a) or C6F4H-p (5b)) as by-products when 2a and 2b were used. 相似文献
32.
Bret B. Wagenhorst Ramji R. Rajendran Ellen E. Van Niel Richard B. Hessler Amir Bukelman Federico Gonzalez-Fernandez 《Journal of molecular evolution》1995,41(5):646-656
Vitamin A and fatty acids are critical to photoreceptor structure, function, and development. The transport of these nutrients between the pigment epithelium and neural retina is mediated by interphotoreceptor retinoid-binding protein (IRBP). IRBP, a 133-kDa (human) glycolipoprotein, is the major protein component of the extracellular matrix separating these two cell layers. In amphibians and mammals, IRBP consists of four homologous repeats of about 300 amino acids which form two retinol and four fatty acid-binding sites. Here we show that IRBP in teleosts is a simpler protein composed of only two repeats. Western blot analysis shows that goldfish IRBP is half the size (70 kDa) of IRBP in higher vertebrates. Metabolic labeling studies employing Brefeldin A taken together with in situ hybridization studies and the presence of a signal peptide show that goldfish IRBP is secreted by the cone photoreceptors. The translated amino acid sequence has a calculated molecular weight of 66.7 kDa. The primary structure consists of only two homologous repeats with a similarity score of 52.5%. The last repeats of human and goldfish IRBPs are 69.1% similar with hydrophobic regions being the most similar. These data suggest that two repeats were lost during the evolution of the ray-finned fish (Actinopterygii), or that the IRBP gene duplicated between the emergence of bony fish (Osteichthyes) and amphibians. Acquisition of a multirepeat structure may reflect evolutionary pressure to efficiently transport higher levels of hydrophobic molecules within a finite space. Quadruplication of an ancestral IRBP gene may have been an important event in the evolution of photoreceptors in higher vertebrates.
Correspondence to: F. Gonzalez-Fernandez 相似文献
33.
Alessandro Sisto Fabio Bonelli Felice Centini Christopher I. Fincham Edoardo Potier Edith Monteagudo Paolo Lombardi Federico Arcamone Cristina Goso Stefano Manzini Alessandro Giolitti Carlo A. Maggi Mariano Venanzi Basilio Pispisa 《Biopolymers》1995,36(4):511-524
In the course of a program aimed at synthesizing novel, potent NK-1 tachykinin receptor antagonists, we developed upon a bioactive model by comparing the low energy structures of a series of peptide and nonpeptide Substance P antagonists. The comparison was based on the super imposition of the aromatic rings, assuming that the rest of the molecule behaves predominantly as a template to arrange the key aromatic groups in the right spatial position. A series of 2-aminocyclohexane carboxylic acid analogues were then selected as the best templates for reproducing the postulated bioactive structure, leading to several pseudo-peptides with interesting biological activity. According to the molecular modeling, these compounds exhibit a neat parallel facing of the indolyl and naphthyl groups at about 3 Å distance. Ultraviolet absorption and steady state fluorescence measurements support this conclusion, showing a linear correlation between the spectral properties and the binding affinity of these analogues. Stacking of the indole ring with naphthalene gives rise to a complex characterized by a well-defined molar extinction coefficient. Consistently, steady state and lifetime fluorescence measurements suggest that the quenching process is ascribable to ground-state interactions between the chromophores. Implications of the π stacking propensity of aromatic groups in the biological activity of the compounds examined are briefly discussed. © 1995 John Wiley & Sons, Inc. 相似文献
34.
35.
Mutations in the X-linked E1 alpha subunit of pyruvate dehydrogenase: exon skipping, insertion of duplicate sequence, and missense mutations leading to the deficiency of the pyruvate dehydrogenase complex. 总被引:4,自引:2,他引:2 下载免费PDF全文
K Chun N MacKay R Petrova-Benedict A Federico A Fois D E Cole E Robertson B H Robinson 《American journal of human genetics》1995,56(3):558-569
Human pyruvate dehydrogenase (PDH)-complex deficiency is an inborn error of metabolism that is extremely heterogeneous in its presentation and clinical course. In a study of 14 patients (7 females and 7 males), we have found a mutation in the coding region of the E1 alpha gene in all 14 patients. Two female patients had the same 7-bp deletion at nt 927; another female patient had a 3-bp deletion at nt 931. Another female patient was found to have a deletion of exon 6 in her cDNA. Two other female patients were found to have insertions, one of 13 bp at nt 981 and one of 46 bp at nucleotide 1078. Two male patients were found to have a 4-bp insertion at nucleotide 1163. The remaining six patients all had missense mutations. A male patient and a female patient both had an A1133G mutation. The other missense mutations were C214T, C615A, and C787G (two patients). Five of these mutations are novel mutations, five have been previously reported in other patients, and two were published observations in other patients in an E1 alpha-mutation summary. In the four cases where parent DNA was available, only one mother was found to be a carrier of the same mutation as her child. 相似文献
36.
M Saceda P García-Morales J M Mato W J Malaisse I Valverde 《Biochemistry international》1984,8(3):445-452
Glucose provokes a transient stimulation of phospholipid methylation in rat pancreatic islets, possibly by increasing phospholipid methyltransferase activity. The association of DL-homocysteine and 3-deazaadenosine inhibits phospholipid methylation. The methylation of phospholipids may play a role in the stimulus-secretion coupling for glucose-induced insulin release. 相似文献
37.
Cesare Peschle Maria Cristina Magli Clemente Cillo Federico Lettieri Francesco Pizzella Arturo Genovese Andrea Soricelli 《Life sciences》1977,21(6):773-778
A single administration of testosterone propionate (TP) in ex-hypoxic polycythemic mice induces, at 24 hr after androgen, an amplification of the erythroid burst-forming unit (BFU-E or B) pool in marrow. This phenomenon is not associated with an amplification of the erythroid colony-forming unit (CFU-E or E) compartment and is followed by its depletion. In the other hand, the 36–49 hr rise of erythropoietin (Ep) levels in serum is followed by a 60-hr amplification of the E pool. It is suggested that the latter phenomenon is mediated by enhanced Ep production, whereas the early amplification of the B compartment may derive from a direct influence of TP at the stem cell level. 相似文献
38.
Germination response to temperature,salinity, light and depth of sowing of ten tropical dune species
This study describes the germination responses of ten tropical dune species to several factors to which their seeds are exposed in the field. Species studied were: three sand dune endemics (Amaranthus greggii, Palafoxia lindenii, and Trachypogon gouini), three pantropical coastal species (Sesuvium portulacastrum, Sporobolus virginicus and Ipomoea stolonifera) and four cosmopolitan grasses also found inland (Panicum repens, Panicum maximum, Pappophorum vaginatum and Andropogon glomeratus). Six species germinated when exposed to different constant temperatures. Four required temperature fluctuation (S. portulacastrum, P. lindenii, S. virginicus, P. repens). Light promoted germination of three species (S. Portulacastrum, P. vaginatum, P. lindenii). Species varied in their degree of sensitivity to salinity. Seeds of T. gouini, I. stolonifera, S. portulacastrum, P. repens, P. Maximum and P. vaginatum were able to germinate under some of the salinity concentrations. Not all species were able to recover after being transferred to distilled water. Seedling emergence was inhibited when seeds were buried. This response was related to depth and to seed size. S. portulacastrum and S. virginicus were the most affected. Nitrates only affected germination response of two species. Seed age promoted germination under a wider range of conditions. P. lindenii showed very heterogeneous responses depending on seed cohort. No dormancy mechanisms were found, other than a thermoperiod and/or light requirement for some of the species. Cosmopolitan grasses tolerated both sand burial and salinity, although the endemics were the most successful in emerging from sand burial; coastal pantropicals were very tolerant to high saline concentrations and recovered completely. 相似文献
39.
M.Carmen Aragón Cecilio Giménez Federico Mayor Juan G. Marvizón Fernando Valdivieso 《生物化学与生物物理学报:生物膜》1981,646(3):465-470
Tyrosine uptake by membrane vesicles derived from rat brain has been investigated. The uptake is dependent on an Na+ gradient ([). The uptake is transport into an osmotically active space and not a binding artifact as indicated by the effect of increasing the medium osmolarity. The process is stimulated by a membrane potential (negative inside) as demonstrated by the effect of the ionophores valinomycin and carbonyl cyanide and anions with different permeabilities. Kinetic data show that tyrosine is accumulated by two systems with different affinities. Tyrosine uptake is inhibited by the presence of phenylalanine and tryptophan. 相似文献
40.
Photosynthetic fructose-1,6-diphosphatase (FDPase) fractions I and II, earlier purified from spinach leaves, show a similar amino acid composition, with the exception of a higher glutamic acid content in the latter. In both fractions glutamic and aspartic acids are the main amino acids. pH activity profiles of fractions I and II are similar, with optima at 8·65–8·70, both showing a high specificity for fructose- 1,6-diphosphate. These two fractions are Mg2+-dependent for activity, with an Optimum Mg2+ concentration of 10 mM in standard conditions, which shifts to 5 mM when the MG2+/EDTA ratio is increased to 10; Mn2+ and Co2+ are slightly active. EDTA enhances FDPase activity slightly, with an optimum at 0·4–0·8 mM. Cysteine has no activating effect, and acts as an inhibitor above 10 mM. Both I and II have an optimum substrate concentration of 4 mM, and the substrate inhibits at concns above this value. Kinetic velocity curves are sigmoidal, with the concave zone located in the range of physiological substrate concns. (Hill coefficient 1·75 for both). This suggests a strong regulatory role of fructose-1,6-diphosphate. Km values are 1·4 × 10−3 M (fraction I) and 1·1 × 10−3 M (fraction II). The highest activity rate occurs at 60°, in accordance with the high thermostability of both fractions; the activation energies are 14·3 kcal/mol (fraction I) and 13·0 kcal/mol (fraction II). 相似文献