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21.
22.
Analysis of Chlamydomonas reinhardtii histones and chromatin 总被引:3,自引:0,他引:3
Chromatin spreads made from isolated nuclei of the unicellular green alga Chlamydomonas reinhardtii show the beaded fibers typical of eukaryotic polynucleosomes. Micrococcal nuclease digestions confirmed the presence of nucleosomes with a repeat length of 189 base pairs, essentially the same as typical mammalian cells. Basic nuclear proteins extracted from isolated nuclei or chromatin with 1 M calcium chloride and 0.3 M hydrochloric acid are resolved into seven major components by electrophoresis in the presence of sodium dodecyl sulfate (SDS). These seven components were subjected to qualitative peptide mapping with V8 protease on SDS gels for comparison with the major histone components of calf thymus. Finally, the C. reinhardtii basic nuclear proteins were fractionated by reversed phase high performance liquid chromatography and their amino acid composition determined. From these studies, we conclude that C. reinhardtii has a full complement of the five histones with properties very similar to those of both higher animals and higher plants. 相似文献
23.
The expression of a highly expressed Bacillus subtilis gene is not reduced by introduction of multiple codons normally not present in such genes 总被引:1,自引:0,他引:1
Charles A. Loshon Federico Tovar-Rojo Susan E. Goldrick Peter Setlow 《FEMS microbiology letters》1989,65(1-2):59-63
A recombinant DNA Proteus mirabilis L-form expression system, LVI (pJS127), was used to synthesize human fusion interferon alpha 1 (f-IFN-alpha 1). In the expression plasmid used, the complete coding sequence of IFN-alpha 1 was linked to the streptococcal speA promoter and the 5' end of the speA structural gene including its signal sequence coding region. LVI (pJS127) was capable of complete secretion into the culture medium of biologically active f-IFN-alpha 1 whose identity was confirmed by immunological and chemical evidence. In particular, bacterial L-forms were for the first time shown to be capable of correct signal peptide processing, as determined by N-terminal sequencing of the secreted f-IFN. 相似文献
24.
Giovanni Fontana Roberto De Bernardi Federico Ferro Anita Gemignani Maurizio Raiteri 《Journal of neurochemistry》1996,66(1):161-168
Abstract: l -Glutamate, NMDA, dl -α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and kainate (KA) increased the release of somatostatin-like immunoreactivity (SRIF-LI) from primary cultures of rat hippocampal neurons. In Mg2+ -containing medium, the maximal effects (reached at ∼100 µ M ) amounted to 737% (KA), 722% (glutamate), 488% (NMDA), and 374% (AMPA); the apparent affinities were 22 µ M (AMPA), 39 µ M (glutamate), 41 µ M (KA), and 70 µ M (NMDA). The metabotropic receptor agonist trans -1-aminocyclopentane-1,3-dicarboxylate did not affect SRIF-LI release. The release evoked by glutamate (100 µ M ) was abolished by 10 µ M dizocilpine (MK-801) plus 30 µ M 1-aminophenyl-4-methyl-7,8-methylenedioxy-5 H -2,3-benzodiazepine (GYKI 52466). Moreover, the maximal effect of glutamate was mimicked by a mixture of NMDA + AMPA. The release elicited by NMDA was sensitive to MK-801 but insensitive to GYKI 52466. The AMPA- and KA-evoked releases were blocked by 6,7-dinitroquinoxaline-2,3-dione (DNQX) or by GYKI 52466 but were insensitive to MK-801. The release of SRIF-LI elicited by all four agonists was Ca2+ dependent, whereas only the NMDA-evoked release was prevented by tetrodotoxin. Removal of Mg2+ caused increase of basal SRIF-LI release, an effect abolished by MK-801. Thus, glutamate can stimulate somatostatin release through ionotropic NMDA and AMPA/KA receptors. Receptors of the KA type (AMPA insensitive) or metabotropic receptors appear not to be involved. 相似文献
25.
Dr. Federico Carbone Prof. Dr. Ruggero Matteucci Dr. Brian R. Rosen Prof. Dr. Antonio Russo 《Facies》1994,30(1):1-13
Summary From a study of two areas, Jesira and the Bajuni Archipelago, about 400 km apart, a general pattern can be established for
the Recent facies, together with the morphological and taxonomic features of the corals. Present day coral development is
characterized by true fringing reefs in the Bajuni Archipelago and by scattered patches and knolls in the Jesira area. The
coral fauna, consisting of 27 genera and 63 species so far (including all uncertainties, but not sight records), is rather
poor, though coral communities are locally well developed. These figures probably reflect incomplete study and sampling. Although
comparison with other areas may therefore be premature, a preliminary biogeographical analysis suggests that this fauna is
more closely related to that of the Red Sea than to East Africa and the Seychelles. This differs from other published biogeographical
work on Indian Ocean coral faunas, but further study of the corals in this and neighbouring areas of the Indian Ocean is needed
in order to resolve this apparent anomaly. 相似文献
26.
John G. Frazier Harry L. Fierstine Sallie C. Beavers Federico Achaval Hiroyuki Suganuma Robert L. Pitman Yuichiro Yamaguchi Carlos Ma. Prigioni 《Environmental Biology of Fishes》1994,39(1):85-96
Synopsis Billfishes have long been known to impale a great variety of objects, but there are only two brief, obscure records of marine
turtles being speared. Details are presented on these two, as well as on two other confirmed records; data from two additional
unconfirmed records are also presented. In total, three species of marine turtles are known to have been impaled by three
species of billfishes; a fourth species of fish and a fourth species turtle are listed in an unconfirmed case. Records come
from the eastern and western Pacific as well as the eastern Atlantic. Of the four confirmed cases, the turtles survived in
two, and apparently died as an effect of the spearing in the other two. In three confirmed cases only the impaled rostrum
was encountered, and in one confirmed case the entire fish was found, with its rostrum piercing the turtle. There is no obvious
advantage — or clear disadvantage — involved in impaling turtles. It is argued that these attacks are accidental, and the
result of attempts made by the billfish to capture prey that are near the turtle. These spearings indicate that the chelonians
serve as shelters for prey animals on the high seas, and thus, are further evidence of the pelagic existence of marine turtles.
The impalings are evidence of a singular ecological role of the turtles — as live fish aggregation devices. 相似文献
27.
Vincenzo La Bella Filippo Brighina Prof. Federico Piccoli Rosa Guarneri 《Neurochemical research》1993,18(2):171-175
Beta-N-oxalylamino-l-alanine (BOAA), a non-protein amino acid present in the seeds of Lathyrus Sativus (LS), is one of several neuroactive glutamate analogs reported to stimulate excitatory receptors and, in high concentrations, cause neuronal degeneration. In the present study, the in vivo acute effects of synthetic BOAA and LS seed extract were investigated on rat cerebellar cyclic GMP following intraperitoneal (10–100 mg/kg) or oral (100 mg/kg) administration of subconvulsive doses of toxin. Furthermore, the BOAA content in LS seeds and in the cerebellum of injected rats was determined by high performance liquid chromatograph analysis. A dose- and time-dependent increase of cerebellar cyclic guanosine monophosphate (cGMP) level was observed after intraperitoneal administration of synthetic BOAA or LS extract. The neurotoxin evoked a maximum stimulation 90 min after injection within the dose range of 50–75 mg/kg, elevating cGMP from basal levels of 5.3±0.5 pmol/mg protein to 15±1.3 pmol/mg protein. Similarly, the oral intake of LS-extracted neurotoxin resulted in the elevation of cGMP content. Kynurenic acid (300 mg/kg i.p.), a non specific excitatory amino acid antagonist, was effective in blocking LS BOAA-elicited cGMP enhancement. The data suggest that in the cerebellum acute administration of low concentrations of BOAA exert in vivo activation of glutamate receptors involved in the regulation of cGMP level. 相似文献
28.
29.
Osmium-fixed, undecalcified, callus tissue from healing fractures of rat tibias was sectioned with a diamond knife for study with the electron microscope. Large multinucleated cells were found adjacent to bone. A characteristic labyrinthine infolded border was consistently seen in parts of the cells close to the bone surface. The innermost parts of this "ruffled border" gave rise to vacuoles. The bone surface was always disrupted under the "ruffled border" of the cells. Needle-like crystals were seen at the osseous fringe, within folds in the ruffled border as well as within vacuoles deeper in the cells. Collagen fibers denuded of crystals were never observed. Mitochondria, containing clusters of fine granules, were abundant. The part of the cell away from bone contained rough endoplasmic reticulum and the cell membrane was thrown into irregular microvilli. These observations are discussed in relation to current concepts of osteoclastic resorption of bone. 相似文献
30.
Federico R Conde M Sandra Churio Carlos M Previtali 《Photochemical & photobiological sciences》2004,3(10):960-967
In vitro studies on the structurally related mycosporine-like amino acids (MAAs) porphyra-334 and shinorine in aqueous solutions were carried out aiming at their full photochemical and photophysical characterization and expanding the evidence on the assigned UV-photoprotective role of the molecules in vivo. The experiments on shinorine confirmed a high photostability and a poor fluorescence quantum yield, in concordance with previous results on porphyra-334. The estimation of triplet production quantum yields for both MAAs was achieved by laser-flash photolysis measurements. In particular, photosensitization experiments on porphyra-334 support the participation of the triplet state in the photodecomposition mechanism yielding a more precise value of [capital Phi](T). As well, photoacoustic calorimetry experiments allowed the first direct quantification of the nonradiative relaxation pathways of the excited MAAs in solution, corroborating that the vast majority (ca. 97%) of the absorbed energy is promptly delivered to the surroundings as heat, consistently with the low photodecomposition and emission yields observed. 相似文献