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241.
G Paganelli G Viale C Rossetti P Magnani F Sudati G Vanoli E Seccamani F Palmucci F Fazio 《Journal of nuclear biology and medicine (Turin, Italy : 1991)》1991,35(1):41-46
A xenograft model of human malignant melanoma was used to compare, in terms of tumor localization, the specific antimelanoma monoclonal antibody (MoAb) 225.28S with an irrelevant antibody (4C4). Both specific and non-specific 99mTc-labeled fragments were injected in 12 nude mice bearing subcutaneous tumor. The animals were then sacrificed at 6 and 24 hours post-injection and immediately dissected. Radioactivity of the tumor and normal tissues was measured in a well scintillation counter and autoradiography of tumor, liver and kidneys was also obtained. Tumor localization of 99mTc-labeled MoAb 225.28S fragments was highly specific compared with 99mTc-labeled irrelevant antibody 4C4. With the exception of the kidneys, already at six hours there was a satisfactory tumor-to-normal tissue ratio, which improved at 24 hours. However, the percentage of injected dose per gram of tumor decreased with time, probably due to the weaker bond of radiolabeled Fab' fragments to tumor cells. These results would indicate 99mTc-fragments of the antimelanoma MoAb 225.28S as a suitable radiotracer in clinical nuclear medicine. 相似文献
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Muktheshwar Gandesiri Saritha Chakilam Jelena Ivanovska Natalya Benderska Matthias Ocker Pietro Di Fazio Maria Feoktistova Hala Gali-Muhtasib Margret Rave-Fränk Olaf Prante Hans Christiansen Martin Leverkus Arndt Hartmann Regine Schneider-Stock 《Apoptosis : an international journal on programmed cell death》2016,21(5):671-674
244.
Martina Padovano Matteo Scopetti Federico Manetti Donato Morena Davide Radaelli Stefano D’Errico Nicola Di Fazio Paola Frati Vittorio Fineschi 《World journal of stem cells》2022,14(8):577-586
The latest achievements in the field of pancreas transplantation and stem cell therapy require an effort by the scientific community to clarify the ethical implications of pioneering treatments, often characterized by high complexity from a surgical point of view, due to transplantation of multiple organs at the same time or at different times, and from an immunological point of view for stem cell therapy. The fundamental value in the field of organ transplants is, of course, a solidarity principle, namely that of protecting the health and life of people for whom transplantation is a condition of functional recovery, or even of survival. The nature of this value is that of a concept to which the legal discipline of transplants entrusts its own ethical dignity and for which it has ensured a constitutional recognition in different systems. The general principle of respect for human life, both of the donor and of the recipient, evokes the need not to put oneself and one’s neighbor in dangerous conditions. The present ethical reflection aims to find a balance between the latest therapeutic advances and several concepts including the idea of the person, the respect due to the dead, the voluntary nature of the donation and the consent to the same, the gratuitousness of the donation, the scientific progress and the development of surgical techniques, and the policies of health promotion. 相似文献
245.
C.E. Henry C. Emmanuel N. Lambie C. Loo B. Kan C.J. Kennedy A. de Fazio N.F. Hacker C.E. Ford 《Translational oncology》2017,10(3):346-356
OBJECTIVE: The ROR1 and ROR2 receptor tyrosine kinases have both been implicated in ovarian cancer progression and have been shown to drive migration and invasion. There is an increasing importance of the role of stroma in ovarian cancer metastasis; however, neither ROR1 nor ROR2 expression in tumor or stromal cells has been analyzed in the same clinical cohort. AIM: To determine ROR1 and ROR2 expression in ovarian cancer and surrounding microenvironment and examine associations with clinicopathological characteristics. METHODS: Immunohistochemistry for ROR1 and ROR2 was used to assess receptor expression in a cohort of epithelial ovarian cancer patients (n = 178). Results were analyzed in relation to clinical and histopathological characteristics and survival. Matched patient sample case studies of normal, primary, and metastatic lesions were used to examine ROR expression in relation to ovarian cancer progression. RESULTS: ROR1 and ROR2 are abnormally expressed in malignant ovarian epithelium and stroma. Higher ROR2 tumor expression was found in early-stage, low-grade endometrioid carcinomas. ROR2 stromal expression was highest in the serous subtype. In matched patient case studies, metastatic samples had higher expression of ROR2 in the stroma, and a recurrent sample had the highest expression of ROR2 in both tumor and stroma. CONCLUSION: ROR1 and ROR2 are expressed in tumor-associated stroma in all histological subtypes of ovarian cancer and hold potential as therapeutic targets which may disrupt tumor and stroma interactions. 相似文献
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Patrícia IS Pinto Pratap B Singh João B Condeça Helena R Teodósio Deborah M Power Adelino VM Canário 《Reproductive biology and endocrinology : RB&E》2006,4(1):67-11
Background
ICI 182,780 (ICI) belongs to a new class of antiestrogens developed to be pure estrogen antagonists and, in addition to its therapeutic use, it has been used to knock-out estrogen and estrogen receptor (ER) actions in several mammalian species. In the present study, the effects and mechanism of action of ICI were investigated in the teleost fish, sea bream (Sparus auratus). 相似文献248.
REDUCING INCIDENTAL MORTALITY OF FRANCISCANA DOLPHIN PONTOPORIA BLAINVILLEI WITH ACOUSTIC WARNING DEVICES ATTACHED TO FISHING NETS 总被引:1,自引:1,他引:0
P. Bordino S. Kraus D. Albareda A. Fazio A. Palmerio M. Mendez S. Botta 《Marine Mammal Science》2002,18(4):833-842
We conducted a double blind experiment in an artisanal gillnet fishery in Argentina to determine the effectiveness of acoustic deterrents (pingers) at reducing bycatch of the Franciscana dolphin ( Pontoporia blainvillei ). The fishery was conducted by small inflatable and fiberglass vessels operating between 0.5 and 7 krn from the coast. Each vessel carried an independent observer who was rotated from vessel to vessel throughout the course of the experiment. Information on the number of dolphins captured, geographic position, depth, configuration of fishing gear, soak time, biomass of fish caught, and sea lion predation in a string/net producing any damage was recorded. Equivalent numbers of active and silent pingers were used during the experiment. A total of 45 dolphins were caught in the silent nets, and seven were caught in the active pinger nets, demonstrating a highly significant reduction in bycatch for this species. However, sea-lions ( Otaria flavescens ) damaged the fish in active pinger nets significantly more than silent nets, and the damage increased over the course of the experiment. Although pingers show promise as a management tool for this species, pinniped depredation suggests that higher pinger frequencies will be needed to avoid a "dinner bell" effect. 相似文献
249.
Alejandra T. Fazio María D. Bertoni Mónica T. Adler Laura B. Ruiz María L. Rosso Lucia Muggia Armin Hager Elfie Stocker-Wörgötter Marta S. Maier 《Mycological Progress》2009,8(4):359-365
A strain of the lichen mycobiont isolated from a thallus of Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were
studied. The isolated fungal colonies developed well and showed a remarkable morphogenetic capacity on most of the assayed
solid media, e.g., malt extract 2%-yeast extract 0.2% (MEYE), malt extract 1%-yeast extract 0.4%-sucrose 10% (MY10), and the
original Lilly & Barnett medium (LB). The identity of the isolated fungus was confirmed by its ITS rDNA-sequence. Atranorin,
the major cortical lichen depside, was produced when the colonies were grown over 5 and 10 months on solid LB medium, combined
with a dessication treatment. Atranorin was identified by matching of UV spectra obtained from HPLC running and a reference
substance in a spectrum library. Colonies grown on MEYE and MY10 with a dessication treatment did not produce any lichen secondary
metabolite. Mycobionts grown for 5 months on solid MEYE without a dessication treatment produced triacylglycerides as the
major metabolites, and the fatty acids were characterized as their methyl esters. Analysis by TLC and HPLC-DAD of extracts
of colonies grown on LB and MY10 without dessication revealed that the typical secondary compounds of the natural lichen were
not produced. The major metabolites of the natural lichen thallus were identified by chromatographic and spectroscopic methods. 相似文献
250.