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51.
Caruana F Jezzini A Sbriscia-Fioretti B Rizzolatti G Gallese V 《Current biology : CB》2011,21(3):195-199
Evidence from a large number of brain imaging studies has shown that, in humans, the insula, and especially its anterior part, is involved in emotions and emotion recognition. Typically, however, these studies revealed that, besides the insula, a variety of other cortical and subcortical areas are also active. Brain imaging studies are correlative in nature, and, as such, they cannot give indications about the necessary contribution of the different centers involved in emotions. In the present study, we aimed to define more clearly the role of the insula in emotional and social behavior of the monkey by stimulating it electrically. Using this technique, one may determine whether direct activation of the insula can produce specific emotional or social behaviors and exactly which parts of this structure are responsible for these behaviors. The results showed that two emotional behaviors, a basic one (disgust) and a social one (affiliative state), were easily elicited by electrical stimulation of specific parts of the insula. Both behaviors were characterized by specific motor and vegetative responses and by a dramatic change in the monkey's responsiveness to external stimuli. 相似文献
52.
Nanopharmaceutics composed of a carrier and a protein have the potential to improve the activity of therapeutical proteins. Therapy for lysosomal diseases is limited by the lack of effective protein delivery systems that allow the controlled release of specific proteins to the lysosomes. Here we address this problem by developing functional polyelectrolyte-based nanoparticles able to promote acidic pH-triggered release of the loaded protein. Trimethyl chitosan (TMC) was synthesized and allowed to form polyelectrolyte complexes (PECs) with the lysosomal enzyme α-GAL through self-assembly and ionotropic gelation, with average particle size <200 nm, polydispersity index (PDI) <0.2, ζ potential of ~ 20 mV, and a protein loading efficiency close to 65%. These polyelectrolyte nanoparticles were stable and active under physiological conditions and able to release the enzyme at acidic pH, as demonstrated by in situ atomic force microscopy (AFM). These nanoparticles were further functionalized with Atto 647N for single-particle characterization and tracking their cellular uptake and fate using high-resolution fluorescence microscopy. In contrast with their precursor, TMC, PECs were efficiently internalized by human endothelial cells and mostly accumulated in lysosomal compartments. The superior physicochemical characteristics of the TMC/α-GAL PECs together with their excellent cellular uptake properties indicate their enormous potential as advanced protein delivery systems for the treatment of lysosomal storage diseases. 相似文献
53.
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55.
We used light microscopy to study placental structure of the lizard Sceloporus mucronatus throughout 6 months of embryonic development. Three stages of placental development could be assigned to embryos based on the arrangement of the extraembryonic membranes. A highly vascular choriovitelline placenta was present in the embryonic hemisphere and a nonvascular bilaminar omphalopleure covered most of the abembryonic hemisphere of the egg during embryonic Stages 10-28. A chorioallantoic placenta replaced the choriovitelline placenta by embryonic Stage 29 and an omphaloplacenta covered the abembryonic hemisphere at this stage. The combination of these two placental types occurred in Stage 29-36 embryos. The final stage of placentation, embryonic Stages 37-40, was characterized by an omphalallantoic placenta in the abembryonic hemisphere and a chorioallantoic placenta in the embryonic hemisphere of the egg. The choriovitelline and chorioallantoic placentae are well vascularized, with closely apposed maternal and embryonic blood vessels. These structures are the most likely sites of respiratory exchange. In contrast, the omphaloplacenta and omphalallantoic placentae contain cuboidal or columnar epithelia and these structures may function in histotrophic exchange. Placentation of S. mucronatus is similar to that of predominantly lecithotrophic species in other squamate lineages suggesting that the evolution of this placental morphology is a response to similar factors and is independent of phylogeny. 相似文献
56.
Effect of ion-binding and chemical phospholipid structure on the nanomechanics of lipid bilayers studied by force spectroscopy
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The nanomechanical response of supported lipid bilayers has been studied by force spectroscopy with atomic force microscopy. We have experimentally proved that the amount of ions present in the measuring system has a strong effect on the force needed to puncture a 1,2-dimyristoyl-sn-glycero-3-phosphocholine bilayer with an atomic force microscope tip, thus highlighting the role that monovalent cations (so far underestimated, e.g., Na(+)) play upon membrane stability. The increase in the yield threshold force has been related to the increase in lateral interactions (higher phospholipid-phospholipid interaction, decrease in area per lipid) promoted by ions bound into the membrane. The same tendency has also been observed for other phosphatidylcholine bilayers, namely, 2-dilauroyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and 1,2-dioleoyl-sn-3-phosphocholine, and also for phosphatidylethanolamine bilayers such as 1-palmitoyl-2-oleoyl-sn-3-phosphoethanolamine. Finally, this effect has been also tested on a natural lipid bilayer (Escherichia coli lipid extract), showing the same overall tendency. The kinetics of the process has also been studied, together with the role of water upon membrane stability and its effect on membrane nanomechanics. Finally, the effect of the chemical structure of the phospholipid molecule on the nanomechanical response of the membrane has also been discussed. 相似文献
57.
Achiridae is an important family of the order Pleuronectiformes widely distributed in North, Central, and South America with
freshwater and marine species. In the present study cytogenetic analyses comprising conventional and molecular techniques
were carried out in seven species of this family. The following diploid numbers (2n) and fundamental numbers (FN) were obtained: Achirus declivis 2n = 34, FN = 52; Achirus lineatus 2n = 40, FN = 66; Catathyridium jenynsi 2n = 40 and FN = 50; Gymnachirus nudus 2n = 36 and FN = 50; Hypoclinemus mentalis 2n = 38 and FN = 54; Trinectes paulistanus 2n = 42 and FN = 52; and Trinectes sp. 2n = 38 and FN = 54. All species presented a single nucleolar organizer region (NOR) bearing chromosome pair and C-band positive
segments mainly distributed at the pericentromeric position. The wide variation observed in chromosome number and FN suggests
the occurrence of larger chromosome rearrangements in the family Achiridae if compared with other families of the same order. 相似文献
58.
The family Loricariidae with about 690 species divided into six subfamilies, is one of the world’s largest fish families.
Recent studies have shown the existence of several problems in the definition of natural groups in the family, which has made
the characterization of the subfamilies and even of some genera quite difficult. With the main objective of contributing for
a better understanding of the relationships between loricariids, cytogenetic analysis were conducted with two species of Neoplecostominae
and nine species of Hypostominae that, according to morphological and molecular data, may belong to a new monophyletic unit.
The results obtained showed a marked chromosomal conservation with the presence of 2n = 54 chromosomes and single interstitial Ag-NORs in all species analyzed. Considering that Neoplecostominae is the primitive
sister-group of all other loricariids, with exception of Lithogeneinae, this karyotypic structure may represent the primitive
condition for the family Loricariidae. The cytogenetic characteristics partaken by the species of Neoplecostominae and Hypostominae
analyzed in the present study reinforce the hypothesis that the species of both these subfamilies might belong to a natural
group. 相似文献
59.
Weigert C Brodbeck K Staiger H Kausch C Machicao F Häring HU Schleicher ED 《The Journal of biological chemistry》2004,279(23):23942-23952
60.
Carneiro FA Lapido-Loureiro PA Cordo SM Stauffer F Weissmüller G Bianconi ML Juliano MA Juliano L Bisch PM Da Poian AT Poian AT 《European biophysics journal : EBJ》2006,35(2):145-154
The entry of enveloped animal viruses into their host cells always depends on membrane fusion triggered by conformational
changes in viral envelope glycoproteins. Vesicular stomatitis virus (VSV) infection is mediated by virus spike glycoprotein
G, which induces membrane fusion between the viral envelope and the endosomal membrane at the acidic environment of this compartment.
In this work, we evaluated VSV interactions with membranes of different phospholipid compositions, at neutral and acidic pH,
using atomic force microscopy (AFM) operating in the force spectroscopy mode, isothermal calorimetry (ITC) and molecular dynamics
simulation. We found that the binding forces differed dramatically depending on the membrane phospholipid composition, revealing
a high specificity of G protein binding to membranes containing phosphatidylserine (PS). In a previous work, we showed that
the sequence corresponding amino acid 164 of VSV G protein was as efficient as the virus in catalyzing membrane fusion at
pH 6.0. Here, we used this sequence to explore VSV–PS interaction using ITC. We found that peptide binding to membranes was
exothermic, suggesting the participation of electrostatic interactions. Peptide–membrane interaction at pH 7.5 was shown to
be specific to PS and dependent on the presence of His residues in the fusion peptide. The application of the simplified continuum
Gouy–Chapman theory to our system predicted a pH of 5.0 at membrane surface, suggesting that the His residues should be protonated
when located close to the membrane. Molecular dynamics simulations suggested that the peptide interacts with the lipid bilayer
through its N-terminal residues, especially Val145 and His148.
Fabiana A.Carneiro and Pedro A. Lapido-Loureiro contributed equally to this work
An erratum to this article can be found at 相似文献