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81.
Fatemeh Sadeghi Monish Kumar Irfan N. Bandey Xiaoyang Li Badrinath Roysam Navin Varadarajan 《Biotechnology and bioengineering》2022,119(1):199-210
Ligand inducible proteins that enable precise and reversible control of nuclear translocation of passenger proteins have broad applications ranging from genetic studies in mammals to therapeutics that target diseases such as cancer and diabetes. One of the drawbacks of the current translocation systems is that the ligands used to control nuclear localization are either toxic or prone to crosstalk with endogenous protein cascades within live animals. We sought to take advantage of salicylic acid (SA), a small molecule that has been extensively used in humans. In plants, SA functions as a hormone that can mediate immunity and is sensed by the nonexpressor of pathogenesis-related (NPR) proteins. Although it is well recognized that nuclear translocation of NPR1 is essential to promoting immunity in plants, the exact subdomain of Arabidopsis thaliana NPR1 (AtNPR1) essential for SA-mediated nuclear translocation is controversial. Here, we utilized the fluorescent protein mCherry as the reporter to investigate the ability of SA to induce nuclear translocation of the full-length NPR1 protein or its C-terminal transactivation (TAD) domain using HEK293 cells as a heterologous system. HEK293 cells lack accessory plant proteins including NPR3/NPR4 and are thus ideally suited for studying the impact of SA-induced changes in NPR1. Our results obtained using a stable expression system show that the TAD of AtNPR1 is sufficient to enable the reversible SA-mediated nuclear translocation of mCherry. Our studies advance a basic understanding of nuclear translocation mediated by the TAD of AtNPR1 and uncover a biotechnological tool for SA-mediated nuclear localization. 相似文献
82.
Samadi Mitra Beigi Laleh Yadegari Fatemeh Ansari Alireza Madjid Majidzadeh-A Keivan Eskordi Maryam Farahmand Leila 《Genetica》2022,150(5):289-297
Genetica - Although predicting the effects of variants near intron-exon boundaries is relatively straightforward, predicting the functional Exon Splicing Enhancers (ESEs) and the possible effects... 相似文献
83.
Nesaei Abolfazl Naderi Ghale-noie Zari Khorshid Shamshiri Asma Afzaljavan Fahimeh Rivandi Mahdi Tajbakhsh Amir Homaei Shandiz Fatemeh Pasdar Alireza 《Molecular biology reports》2022,49(5):3549-3557
Molecular Biology Reports - Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease... 相似文献
84.
Pourmoghadasiyan Bahareh Tavakkoli Fatemeh Beram Farzaneh Mahmoudi Badmasti Farzad Mirzaie Amir Kazempour Reza Rahimi Shahrzad Larijani Setare Farokhi Hejabi Faranak Sedaghatnia Kamand 《Molecular biology reports》2022,49(5):3597-3608
Molecular Biology Reports - In this study, the optimized niosomal formulation containing paclitaxel using non-ionic surfactants and cholesterol was designed and its cytotoxic effects against... 相似文献
85.
rafiei Ali Khodabakhsh Farnaz Cohan Reza Ahangari Azadegan-Dehkordi Fatemeh Salimian Morteza 《Molecular biology reports》2022,49(5):3389-3399
Molecular Biology Reports - Aberrant expression of long non-coding RNAs (lncRNAs) can contribute to the pathogenesis of coronary artery disease (CAD). In this study, we aimed to evaluate the... 相似文献
86.
Yousefi Fatemeh Najafi Hadi Behmanesh Mehrdad Soltani Bahram M. 《Molecular biology reports》2022,49(5):3377-3387
Molecular Biology Reports - Aberrant activation of the Wnt signaling pathway is observed in most colorectal cancers (CRC). OCC-1D is a splice variant of OCC-1 gene which is considered as a long... 相似文献
87.
BioMetals - The toxic nature of arsenic has left a trail of disastrous health consequences around the world. Microorganisms have developed various strategies to deal with arsenic. The presence of... 相似文献
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An electrodeposition method was applied to form gold-platinum (AuPt) alloy nanoparticles on the glassy carbon electrode (GCE) modified with a mixture of an ionic liquid (IL) and chitosan (Ch) (AuPt-Ch-IL/GCE). AuPt nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and electrochemical methods. AuPt-Ch-IL/GCE electrocatalyzed the reduction of H(2)O(2) and thus was suitable for the preparation of biosensors. Cholesterol oxidase (ChOx) was then, immobilized on the surface of the electrode by cross-linking ChOx and chitosan through addition of glutaraldehyde (ChOx/AuPt-Ch-IL/GCE). The fabricated biosensor exhibited two wide linear ranges of responses to cholesterol in the concentration ranges of 0.05-6.2 mM and 6.2-11.2 mM. The sensitivity of the biosensor was 90.7 μA mM(-1) cm(-2) and the limit of detection was 10 μM of cholesterol. The response time was less than 7 s. The Michaelis-Menten constant (K(m)) was found as 0.24 mM. The effect of the addition of 1 mM ascorbic acid and glucose was tested on the amperometric response of 0.5 mM cholesterol and no change in response current of cholesterol was observed. 相似文献