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141.
142.
The present investigation reports, the synthesis of manganese oxide (α-Mn2O3) nanobundles using thermal decomposition and its physicochemical characterization. The α-Mn2O3 nanobundles have been prepared using manganese oxalate dihydrate powders as precursor in the presence of oleylamine and triphenylphosphine as solvent and capping agent. Transmission electron microscopy (TEM) analysis demonstrated Mn2O3 nanobundles compose of nanospheres with diameter 30 nm. The products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT-IR) spectroscopy. Manganese oxide nanocrystals have been prepared under different condition. The controlled experimental results showed that the use of oleylamine and triphenylphosphine as the solvent and capping agent in the chemical process played important role in the formation of the final products. 相似文献
143.
Fatemeh Rafii Miseon Park Gonçalo Gamboa da Costa Luisa Camacho 《Archives of microbiology》2009,191(12):895-902
The production of short-chain fatty acids, reductive enzymes, and hydrolytic enzymes by four gatifloxacin-selected, fluoroquinolone-resistant,
mutant strains of C. perfringens, with stable mutations either in DNA gyrase or in both DNA gyrase and topoisomerase IV, was compared with that produced by
the wild-type parent strains to investigate the effect of mutations associated with the selection of gatifloxacin resistance
on bacterial metabolic activities. The mutants differed from their respective wild-type parent strains in the enzymatic activities
of azoreductase, nitroreductase, and β-glucosidase and in the ratio of butyric acid to acetic acid production. Microarray
analysis of one wild type and the corresponding mutant revealed different levels of mRNA expression for the enzymes involved
in short-chain fatty acid (SCFA) synthesis and for β-glucosidase and oxidoreductases. In addition to mutations in the target
genes, selection of resistance to gatifloxacin resulted in strain-specific physiological changes in the resistant mutants
of C. perfringens that affected their metabolic activities. 相似文献
144.
An isoenzyme analysis was performed on 109 individuals from 11 populations of Juniperus excelsa complex collected from Iran. A total of 61 allozymes from five enzyme systems were scored. The mean numbers of bands per presumed isoenzyme, ranged from 2.07 to 3.23. The value of Euclidean distance ranged from 1.920 to 4.411. The average value of Shannon diversity index (H′) estimated for each population ranged from 0.317 in the population sampled from ‘Fasa’ to 1.053 in the population of ‘Golestan’, and the average H′ value for each enzyme system ranged from 0.031 (SOD-3) to 1.670 (PPO-1). To reveal the relationships among these populations, a cluster analysis based on Euclidean distances was performed. The results of the analysis suggest recognizing three taxa in the complex in Iran: Juniperus polycarpos subsp. polycarpos, Juniperus polycarpos subsp. turcomanica, and one cryptic species not recorded from Iran until now. Moreover, the analysis presented, does not confirm the occurrence of J. excelsa in Iran. 相似文献
145.
Soltani F Mosaffa F Iranshahi M Karimi G Malekaneh M Haghighi F Behravan J 《Cell biology and toxicology》2009,25(3):291-296
The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested.
Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H2O2-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay).
Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 μM) alone or a combination of different concentrations
of UMB (10, 25, 50, 100, 200, and 400 μM) and 25 μM H2O2. Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 μM) and H2O2 (25 μM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively.
Single cells were analyzed with “TriTek Cometscore version 1.5” software. The DNA damage was expressed as percent tail DNA.
UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 μM H2O2 (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0–50 μM, was greater than that of UMB. However, no
significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 μM. 相似文献
146.
Fatemeh Jamali Abbas Sharifi-Tehrani Matthias P. Lutz Monika Maurhofer 《Microbial ecology》2009,57(2):267-275
The production of hydrogen cyanide (HCN) and 2,4-diacetylphloroglucinol (DAPG) is a major factor in the control of soil-borne
diseases by Pseudomonas fluorescens CHA0. We investigated the impact of different biotic factors on the expression of HCN–in comparison to DAPG biosynthetic
genes in the rhizosphere. To this end, the influence of plant cultivar, pathogen infection, and coinoculation with other biocontrol
strains on the expression of hcnA-lacZ and phlA-lacZ fusion in strain CHA0 was monitored on the roots of bean. Interestingly, all the tested factors influenced the expression
of the two biocontrol traits in a similar way. For both genes, we observed a several-fold higher expression in the rhizosphere
of cv. Derakhshan compared with cvs. Goli and Naz, although bacterial rhizosphere colonization levels were similar on all
cultivars tested. Root infection by Rhizoctonia solani stimulated total phlA and hcnA gene expression in the bean rhizosphere. Coinoculation of strain CHA0 with DAPG-producing P. fluorescens biocontrol strains Pf-68 and Pf-100 did neither result in a substantial alteration of hcnA nor of phlA expression in CHA0 on bean roots. To our best knowledge, this is the first study investigating the impact of biotic factors
on HCN production by a bacterial biocontrol strain in the rhizosphere. 相似文献
147.
Secondary Metabolites Produced by the Marine Bacterium Halobacillus salinus That Inhibit Quorum Sensing-Controlled Phenotypes in Gram-Negative Bacteria 下载免费PDF全文
Margaret E. Teasdale Jiayuan Liu Joselynn Wallace Fatemeh Akhlaghi David C. Rowley 《Applied microbiology》2009,75(3):567-572
Certain bacteria use cell-to-cell chemical communication to coordinate community-wide phenotypic expression, including swarming motility, antibiotic biosynthesis, and biofilm production. Here we present a marine gram-positive bacterium that secretes secondary metabolites capable of quenching quorum sensing-controlled behaviors in several gram-negative reporter strains. Isolate C42, a Halobacillus salinus strain obtained from a sea grass sample, inhibits bioluminescence production by Vibrio harveyi in cocultivation experiments. With the use of bioassay-guided fractionation, two phenethylamide metabolites were identified as the active agents. The compounds additionally inhibit quorum sensing-regulated violacein biosynthesis by Chromobacterium violaceum CV026 and green fluorescent protein production by Escherichia coli JB525. Bacterial growth was unaffected at concentrations below 200 μg/ml. Evidence is presented that these nontoxic metabolites may act as antagonists of bacterial quorum sensing by competing with N-acyl homoserine lactones for receptor binding. 相似文献
148.
149.
Yurong Xin Benjamin Chanrion Meng-Min Liu Hanga Galfalvy Ramiro Costa Boro Ilievski Gorazd Rosoklija Victoria Arango Andrew J. Dwork J. John Mann Benjamin Tycko Fatemeh Haghighi 《PloS one》2010,5(6)
Background
Emerging evidence suggests that DNA methylation plays an expansive role in the central nervous system (CNS). Large-scale whole genome DNA methylation profiling of the normal human brain offers tremendous potential in understanding the role of DNA methylation in brain development and function.Methodology/Significant Findings
Using methylation-sensitive SNP chip analysis (MSNP), we performed whole genome DNA methylation profiling of the prefrontal, occipital, and temporal regions of cerebral cortex, as well as cerebellum. These data provide an unbiased representation of CpG sites comprising 377,509 CpG dinucleotides within both the genic and intergenic euchromatic region of the genome. Our large-scale genome DNA methylation profiling reveals that the prefrontal, occipital, and temporal regions of the cerebral cortex compared to cerebellum have markedly different DNA methylation signatures, with the cerebral cortex being hypermethylated and cerebellum being hypomethylated. Such differences were observed in distinct genomic regions, including genes involved in CNS function. The MSNP data were validated for a subset of these genes, by performing bisulfite cloning and sequencing and confirming that prefrontal, occipital, and temporal cortices are significantly more methylated as compared to the cerebellum.Conclusions
These findings are consistent with known developmental differences in nucleosome repeat lengths in cerebral and cerebellar cortices, with cerebrum exhibiting shorter repeat lengths than cerebellum. Our observed differences in DNA methylation profiles in these regions underscores the potential role of DNA methylation in chromatin structure and organization in CNS, reflecting functional specialization within cortical regions. 相似文献150.
An overview of Iranian mangrove ecosystems, northern part of the Persian Gulf and Oman Sea 下载免费PDF全文
Mohammad Ali Zahe Fatemeh Rouhani Soraya Mohajeri Farshid Bateni Leila Mohajeri 《农业工程》2010,30(4):240-244
Iranian mangrove forests occur between longitude 25°19′ and 27°84′, in the north part of the Persian Gulf and Oman Sea. In 2002, it was estimated that 93.37 km2 of Iranian shorelines were covered with mangrove forests, with the largest area (67.5 km2) occurring between the Khamir Port and the northwest side of Qeshm Island, and the smallest area (0.01 km2) in the Bardestan estuary. Only two species of mangrove are found in the Persian Gulf: Avicennia marina from Avicenniaceae and Rhizophora macrunata from Rhizophoraceae. A. marina is the dominant specie in these forests whereas Rh. macrunata is found only in the Sirik region. Overexploitation of mangrove leaves and oil pollution are the main causes of mangrove destruction in this region. 相似文献