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91.
Anum Jazba O’Shea Charlotte Zeeshan Hyder M. Farrukh Sumaira Skriver Karen Malik Saad Imran Yasmin Tayyaba 《Molecular biology reports》2022,49(1):293-302
Molecular Biology Reports - Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential... 相似文献
92.
Farrukh A. Shamsi Seema Husain S. M. Hadi 《Journal of biochemical and molecular toxicology》1996,11(2):67-71
Uric acid is present in human plasma in relatively high concentrations and is considered to be a natural physiological antioxidant. We have earlier shown that in the presence of Cu(II) and molecular oxygen, uric acid causes strand breakage in DNA. In this article, we show that uric acid fluorescence is quenched by addition of DNA, indicating the formation of uric acid-DNA complex. Uric acid-Cu(II)-mediated DNA strand scission is capable of bacteriophage inactivation and such inactivation is mediated through reduction of Cu(II) to Cu(I) and the generation of oxygen-derived radicals. It is indicated that the DNA breakage is repaired in E. coli and involves the repair of DNA polymerase. © 1996 John Wiley & Sons, Inc. 相似文献
93.
Samavia Zafar Huma Ajab Zaib-un-nisa Mughal Jawaid Ahmed zai Sofia Baig Ayesha Baig Zeshan Habib Farrukh Jamil Muhammad Ibrahim Sumaira Kanwal Muhammad Asif Rasheed 《Saudi Journal of Biological Sciences》2022,29(2):1092-1099
Salmonella enteric serovar Typhimurium is the most common enteric pathogen in humans and animals. Consumption of contaminated food or water triggers inflammation that allows Salmonella to spread into the gut and causes gastrointestinal diseases. The infection spreads by intestinal invasion, phagocyte internalization and subsequent dissemination in many other patients. This research used TolA, a Salmonella typhimurium membrane protein, to computationally design a multi-epitope vaccine against the pathogen. Complete consistency of the candidate vaccine was checked In silico, and molecular dynamics simulations confirmed the vaccine's stability. According to docking report, the vaccine has a good affinity with toll-like receptors. In silico cloning and codon optimization techniques improved the vaccine's efficacy in Salmonella typhimurium manifestation process. The candidate vaccine induced an efficient immune response, as determined by In silico immune simulation. Computational studies revealed that the engineered multi-epitope vaccine is structurally stable, capable of eliciting particular immunological reactions, and therefore a candidate for a latent Salmonella typhimurium vaccine. However, wet lab studies and further investigations are required to confirm the results. 相似文献
94.
Adnan Raza Muhammad Asif Rasheed Sohail Raza Muhammad Tariq Navid Amna Afzal Farrukh Jamil 《Saudi Journal of Biological Sciences》2022,29(4):3006
Newcastle disease virus (NDV), an avian orthoavulavirus, is a causative agent of Newcastle disease named (NDV), and can cause even the epidemics when disease is not treated. Previously several vaccines based on attenuated and inactivated viruses have been reported which are rendered useless with the passage of time due to versatile changes in viral genome. Therefore, we aimed to develop an effective multi-epitope vaccine against the haemagglutinin neuraminidase (HN) protein of 26 NDV strains from Pakistan through a modern immunoinformatic approaches. As a result, a vaccine chimaera was constructed by combining T-cell and B-cell epitopes with the appropriate linkers and adjuvant. The designed vaccine was highly immunogenic, non-allergen and antigenic; therefore, the potential 3D-structureof multi epitope vaccine was constructed, refined and validated. A molecular docking study of a multiepitope vaccine candidate with the chicken Toll-like receptor-4 indicated successful binding. An In silico immunological simulation was used to evaluate the candidate vaccine''s ability to elicit an effective immune response. According to the computational studies, the proposed multiepitope vaccine is physically stable and may induce immune responses whichsuggested it a strong candidate against 26 Newcastle disease virus strains from Pakistan. 相似文献
95.
Naila Abbas Sabir Hussain Farrukh Azeem Tanvir Shahzad Sajjad Haider Bhatti Muhammad Imran Zulfiqar Ahmad Zahid Maqbool Muhammad Abid 《World journal of microbiology & biotechnology》2016,32(11):181
Microbial biotechnologies for the decolorization of textile wastewaters have attracted worldwide attention because of their economic suitability and easiness in handling. However, the presence of high amounts of salts and metal ions in textile wastewaters adversely affects the decolorization efficiency of the microbial bioresources. In this regard, the present study was conducted to isolate salt tolerant bacterial strains which might have the potential to decolorize azo dyes even in the presence of multi-metal ion mixtures. Out of the tested 48 bacteria that were isolated from an effluent drain, the strain NA6 was found relatively more efficient in decolorizing the reactive yellow-2 (RY2) dye in the presence of 50 g L?1 NaCl. Based on the similarity of its 16S rRNA gene sequence and its position in a phylogenetic tree, this strain was designated as Proteus sp. NA6. The strain NA6 showed efficient decolorization (>90 %) of RY2 at pH 7.5 in the presence of 50 g L?1 NaCl under static incubation at 30 °C. This strain also had the potential to efficiently decolorize other structurally related azo dyes in the presence of 50 g L?1 NaCl. Moreover, Proteus sp. NA6 was found to resist the presence of different metal ions (Co+2, Cr+6, Zn+2, Pb+2, Cu+2, Cd+2) and was capable of decolorizing reactive dyes in the presence of different levels of the mixtures of these metal ions along with 50 g L?1 NaCl. Based on the findings of this study, it can be suggested that Proteus sp. NA6 might serve as a potential bioresource for the biotechnologies involving bioremediation of textile wastewaters containing the metal ions and salts. 相似文献
96.
Ensuring adequate food production is a major issue in the context of an increasing human population, limit to the areas of new land that can be cultivated, and loss of existing cultivated lands to abiotic stresses. Of these stresses, salinity consistently has the greatest impact in reducing the area of cultivated land, often due to inappropriate irrigation techniques. To increase food supply, there is a need to produce salt-tolerant crops, which can grow successfully on salt-affected lands. Among crops, vegetables possess a central position in the human diet because of their nutritional value providing vitamins, carbohydrates, proteins, and mineral nutrients. There are many vegetable crops of local importance around the world but others that are very widely cultivated. All of these vegetable crops are affected by salinity more or less severely. Salinity affects every aspect of vegetable crop development including their morphology, physiological function and yield. Although efforts have been made to understand the mechanisms of salt tolerance in vegetable crops, less attention has been paid to these than to the staple crops. Where attempts have been made to improve salt tolerance of vegetables, the strategies have ranged from exogenous application of fertilizers, compatible solutes or plant growth regulators, to use of advanced molecular techniques for genetic modifications. This review focuses on the responses of pea, okra, tomato, eggplant, pepper, carrot, broccoli, cauliflower, and potato to salt stress and the strategies being used to enhance their salt tolerance. 相似文献
97.
98.
Plant serine protease inhibitors are defense proteins crafted by nature for inhibiting serine proteases. Use of eco-friendly, sustainable and effective protein molecules which could halt or slow down metabolism of nutrients in pest would be a pragmatic approach in insect pest management of crops. The host-pest complexes that we observe in nature are evolutionary dynamic and inter-depend on other defense mechanisms and interactions of other pests or more generally speaking symbionts with the same host. Insects have co-evolved and adapted simultaneously, which makes it necessary to investigate serine protease inhibitors in non-host plants. Such novel serine protease inhibitors are versatile candidates with vast potential to overcome the host inhibitor-insensitive proteases. In a nutshell exploring and crafting plant serine proteinase inhibitors (PIs) for controlling pests effectively must go on. Non-host PI seems to be a better choice for coevolved insensitive proteases. Transgenic plants expressing wound inducible chimaeric PIs may be an outstanding approach to check wide spectrum of gut proteinases and overcome the phenomenon of resistance development. Thus, this article focuses on an entire array of plant serine protease inhibitors that have been explored in the past decade, their mode of action and biological implications as well as applications. 相似文献
99.
100.
Farrukh Hussain Ilyas Iqbal Mufakhirah Jan Durrani 《云南植物研究》2006,28(3):305-314
Ethnobotanical study of plants revealed that the local community of Ghalegy, District Swat, invariably uses 126 species of 59 families for various purposes. Based on their traditional local uses, fifty-seven species (45.2%) were classifted as medicinal, forty-seven as fire wood (37.3 % ), forty-five as forage (35.7 % ), twenty-eight as honey bee species (22.2%), twenty-seven as vegetable species (21.4%), 25 as edible fruits (14 wild and llcultivated), thirteen as timberwood (10.3%), twelve as ornamental (9.5%), eleven as furniture wood, ten as shelter and thatch makers (7.9%), ten as fencing (7.9%), five as poisonous (4%), four as religious/superstitious species, three species used in making hand sticks and wooden tools (2.4%), three as utensil cleaner species (2.4%), three as evil repellent and one as fish poisoning species. The area is in plant resources and traditional knowledge but it needs ecological management for its sustainability. 相似文献