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排序方式: 共有85条查询结果,搜索用时 15 毫秒
61.
62.
Moiz Ahmed Khan Kehkashan Imtiaz Humaira Shafaq Joveria Farooqi Mohammad Hassan Afia Zafar Maureen T. Long Kelli L. Barr Erum Khan 《中国病毒学》2022,37(5):774-777
Highlights:
1 Potential transfusion-related transmission of DENV, WNV and JEV was investigated in healthy blood donors from the blood bank of Aga Khan University during July to December 2018.
2 ELISA for DENV, WNV and JEV IgM antibodies and RT-PCR for viral RNA detection were performed.
3 Of the 360 blood donors screened, IgM antibodies for DENV and WNV were positive in 3.9% and JEV in 0.28% respectively while none of the blood donors tested positive for RT-PCR.
4 Majority of the seropositive donors were between 19 to 30 years of age and residents of urban areas, mainly from Karachi City.
5 DENV and WNV seropositivity were significantly associated with residence in Malir District of Karachi. 相似文献
1 Potential transfusion-related transmission of DENV, WNV and JEV was investigated in healthy blood donors from the blood bank of Aga Khan University during July to December 2018.
2 ELISA for DENV, WNV and JEV IgM antibodies and RT-PCR for viral RNA detection were performed.
3 Of the 360 blood donors screened, IgM antibodies for DENV and WNV were positive in 3.9% and JEV in 0.28% respectively while none of the blood donors tested positive for RT-PCR.
4 Majority of the seropositive donors were between 19 to 30 years of age and residents of urban areas, mainly from Karachi City.
5 DENV and WNV seropositivity were significantly associated with residence in Malir District of Karachi. 相似文献
63.
A. H. Abad Farooqi Y. N. Shukla Srikant Sharma F. Bangerth 《Plant Growth Regulation》1989,8(3):225-232
In the tubers of medicinal yam (Dioscorea floribunda Mart. & Gal.) abscisic acid (ABA) content was high in all the parts during the dormant condition in the winter, but it decreased to a very low level in the actively growing plants. ABA content of the entire tuber was negatively correlated with temperature and photoperiod. Growth inhibitors including batatasin-I and phthalic acid were identified in the dormant tuber.CIMAP Communication No. 673. 相似文献
64.
M. Farooqi P. Sosnitza M. Saleemuddin R. Ulber T. Scheper 《Applied microbiology and biotechnology》1999,52(3):373-379
A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described.
Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated
by ammonium sulphate fractionation followed by ion-exchange chromatography. Immobilization of glucose oxidase and horseradish
peroxidase was achieved by initially binding the enzymes to a Sepharose matrix coupled with IgG isolated from anti-(glucose
oxidase) and anti-(horseradish peroxidase) sera, respectively. This was followed by alternate incubation with the IgG and
the enzyme to assemble layers of enzyme and antibody on the support. The immunoaffinity-layered preparations obtained thus
were highly active and, after six binding cycles, the amount of enzyme immobilized could be raised about 25 times over that
bound initially. It was also possible to assemble layers of glucose oxidase using unfractionated antiserum in place of the
IgG. The bioaffinity-layered preparations of glucose oxidase and horseradish peroxidase exhibited good enzyme activities and
improved resistance to heat-induced inactivation. The sensitivity of a flow injection analysis system for measuring glucose
and hydrogen peroxide could be remarkably improved using immunoaffinity-layered glucose oxidase and horseradish peroxidase.
For the detection of glucose, a Clark-type oxygen electrode, constructed as a small flow-through cell integrated with a cartridge
bearing immunoaffinity-layered glucose oxidase was employed. The hydrogen peroxide concentration was analysed spectrophotometrically
using a flow-through cell and the layered horseradish peroxidase packed into a cartridge. The immunoaffinity-layered enzymes
could be conveniently solubilized at acid pH and fresh enzyme loaded onto the support. Immunoaffinity-layered glucose oxidase
was successfully used for the on-line monitoring of the glucose concentration during the cultivation of Streptomyces cerevisiae.
Received: 16 November 1998 / Received revision: 22 March 1999 / Accepted: 26 March 1999 相似文献
65.
ABSTRACT: Cancer is a multifaceted molecular disorder that is modulated by a combination of genetic, metabolic and signal transduction aberrations, which severely impair the normal homeostasis of cell growth and death. Accumulating findings highlight the fact that different genetic alterations, such as mutations in tumor suppressor genes, might be related to distinct and differential sensitivity to targeted therapies. It is becoming increasingly apparent that a multipronged approach that addresses genetic milieu (alterations in upstream and/or parallel pathways) eventually determines the response of individual tumors to therapy. Cancerous cells often acquire the ability to evade death by attenuating cell death pathways that normally function to eliminate damaged and harmful cells. Therefore impaired cell death nanomachinery and withdrawal of death receptors from cell surface are some of major determinants for the development of chemotherapeutic resistance encountered during treatment. It is therefore essential to emphasize underlying factors which predispose cells to refractoriness against TRAIL mediated cell death pathway and the relevant regulatory components involved. We bring to limelight the strategies to re-sensitize TRAIL resistant cells via vitamins to induce apoptosis. 相似文献
66.
Aliki Perdikari Tessa Cacciottolo Elana Henning Edson Mendes de Oliveira Julia M. Keogh I. Sadaf Farooqi 《Open biology》2022,12(3)
Obesity, defined as an excess of adipose tissue that adversely affects health, is a major cause of morbidity and mortality. However, to date, understanding the structure and function of human adipose tissue has been limited by the inability to visualize cellular components due to the innate structure of adipocytes, which are characterized by large lipid droplets. Combining the iDISCO and the CUBIC protocols for whole tissue staining and optical clearing, we developed a protocol to enable immunostaining and clearing of human subcutaneous white adipose tissue (WAT) obtained from individuals with severe obesity. We were able to perform immunolabelling of sympathetic nerve terminals in whole WAT and subsequent optical clearing by eliminating lipids to render the opaque tissue completely transparent. We then used light sheet confocal microscopy to visualize sympathetic innervation of human WAT from obese individuals in a three-dimensional manner. We demonstrate the visualization of sympathetic nerve terminals in human WAT. This protocol can be modified to visualize other structures such as blood vessels involved in the development, maintenance and function of human adipose tissue in health and disease. 相似文献
67.
68.
Farooqi IS 《Cell metabolism》2011,14(1):7-8
The field of vascular molecular imaging is searching for the "holy grail" of an imaging technique that will quantitatively and reliably assess vulnerable coronary plaques. Fluorescence imaging with indocyanine green specifically identifies lipid-rich plaques in rabbits and in humans and represents a promising, though invasive, approach. 相似文献
69.
Farooqi AA Rana A Riaz AM Khan A Ali M Javed S Mukhtar S Minhaj S Rao JR Rajpoot J Amber R Javed FA Waqar-Un-Nisa Khanum R Bhatti S 《Molecular biology reports》2012,39(4):4909-4914
The astonishing development of broad genomics and proteomics tools have catalyzed a new era in both therapeutic interventions and nutrition in prostate cancer. The terms pharmacogenomics and nutrigenomics have been derived out of their genetic forbears as large-scale genomics technologies have been established in the last decade. It is unquestionable that rationale of both disciplines is to individualize or personalize medicine and food and nutrition, and eventually health, by tailoring the drug or the food to the individual genotype. The purpose of this review is to significantly inspect results from current research concerning the mechanisms of action of phytonutrients and potential effects on prostate cancer. Substantial emerging data supports the synergistic adiministration of nutraceuticals with TRAIL in prostate cancer progression to circumvent TRAIL refractoriness. Nonetheless, developing novel scientific methods for discovery, validation, characterization and standardization of these multicomponent phyto-therapeutics is vital to their recognition into mainstream medicine. The key to interpret a personalized response is a greater comprehension of nutrigenomics, proteomics and metabolomics. 相似文献
70.