Insulin-like factor binding protein-3 promotes the G1 cell cycle arrest in several cancer cell lines

Insulin-like growth factor binding protein-3 (IGFBP-3) is a multi-functional protein known to induce apoptosis of various cancer cells in an insulin-like growth factor (IGF)-dependent and IGF-independent manner. In our previous study, we found that IGFBP-3 induced apoptosis through the activation of caspases in 786-O cells. In this study, we further examined that whether IGFBP-3 induced apoptosis through the induction of cell cycle arrest in 786-O, A549 and MCF-7 cells. Our results showed that overexpressed IGFBP-3 resulted in typical apoptotic ultrastructures in A549 cells under transmission electron microscope. The result of flow cytometry analysis indicated that IGFBP-3 arrested the cell cycle at G1-S phase in 786-O, A549 and MCF-7 cells. In A549 cells, quantitative real-time PCR and Western blot analysis showed a significant change in the expression of cell cycle-regulated proteins—a decrease in cyclin E1 expression, an increase in p21 expression. These results indicate a possible mechanism for G1 cell cycle arrest by IGFBP-3. Taken together, cyclin E1 and p21 may play important roles in the IGFBP-3-inducing G1 cell cycle arrest and apoptosis in several human cancer cells.     

三江源大型食草动物数量无人机自动监测方法

应用无人机进行动物调查的研究越来越多,但是缺乏有效的自动处理图像方法,使得目视解释的工作量较大。为研究快速与准确估算三江源区域大型食草动物数量的方法,本文使用两种无人机进行7个站点的遥感影像采集。首先对无人机影像进行灰度化,使矩阵维数下降但梯度信息仍然保留,使运算速度大幅度提高;其次对影像开展高斯滤波,高斯滤波将数据进行能量转化,排除掉属于低能量部分的噪声;第三开展阈值处理得到二值化图像;采用样本中动物形态学特征开展形态运算,先用开运算消除小物体尽可能排除干扰,同时不误删牲畜,再用闭运算排除小型黑洞将同一对象连通不重复计数;最后从二值图像中检索轮廓,并返回检测到的轮廓的个数;从而自动获得主要大型食草动物物种数量与空间分布。精度检验方法为手工计数与自动计数结果比较,相对误差3.1%~6.5%,大多数情况均可达到此水平。采用计算机自动处理图像后,每张图像处理和计数的平均时间小于3 s。无人机影像的自动处理方法可为今后大规模进行藏羊、牦牛、西藏野驴和藏原羚等动物调查提供一种有效、可靠的技术途径。     

沙土鼠脑缺血和再灌流后钙调素蛋白激酶Ⅱ活性变化及其与脑电图的关系

结扎蒙古沙土鼠双侧颈总动脉制作急性脑缺血模型,观察了急性脑缺血和再灌流后钙调素依赖性蛋白激酶Ⅱ(CaM-PKⅡ)和蛋白磷酸酶(CaM-PrP)活性的变化以及脑电图(EEG)的表现。结果表明:(1)CaM-PKⅡ活性随缺血时间延长而逐渐降低,缺血10min酶活性即显著降低;(2)缺血10min再灌流,CaM～PKⅡ活性可部分恢复;(3)急性脑缺血时CaM-PrP活性无明显改变;(4)缺血10min再灌流,EEG在1h基本恢复正常,缺血20min再灌流,EEG在3h仍未恢复正常。上述结果提示:CaM-PKⅡ活性对缺血非常敏感,而CaM-PrP活性对缺血不敏感,脑缺血再灌流后EEG的表现与CaM-PKⅡ活性有一定的相关性。     

Single-Cell Sequencing of Brain Cell Transcriptomes and Epigenomes

1. Download : Download high-res image (161KB)
2. Download : Download full-size image