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61.
An epithermal neutron activation method is used to determine the concentration of mineral elements in human dental enamel.
A large number (252) of samples from ancient and modern origins are analyzed. The analytical results are mathematically processed
using a statistical multivariant method. This allows to differentiate deciduous from permanent teeth and decayed from sound
enamel. It is also possible to distinguish the teeth coming from two different necropoles. The origin and the localization
of determined elements in the mineralized part, or in the aqueous-organic part, of enamel is suggested. Their role, as witnessed
in the physiopathological phenomena of dental enamel, is discussed. 相似文献
62.
以往研究流域开发战略问题,多从流域内资源的开发利用方面考虑,而衡量其方案的优劣也多以经济效益的大小为准绳。现在,环境污染与生态危机已威胁着人类的生存与发展,因此在研究流域战略问题时,必须开拓思路, 相似文献
63.
31例非特异性阴道炎病人阴道微生物群的研究 总被引:2,自引:2,他引:0
本文对31例非特异性阴道炎病人和31例正常人阴道微生物群进行定性、定量分析.结果表明,阴道炎病人的阴道乳杆菌的平均浓度明显低于正常人,而肠杆菌科、葡萄球菌、棒状杆菌、类杆菌属、支原体却明显增多,阴道炎病人未发现特异性病原体,菌群失调可能是其发病因素。乳杆菌为阴道正常优势菌,对改善阴道的微环境,防止条件致病菌引起的内源性感染具有重要的生理作用。 相似文献
64.
Swelling and Ca2+-activated Anion Conductances in C127 Epithelial Cells Expressing WT and ΔF508-CFTR
CFTR is a chloride channel that is required for fluid secretion and salt absorption in many exocrine epithelia. Mutations
in CFTR cause cystic fibrosis. CFTR expression influences some ion channels, but the range of channels influenced, the mechanism
of the interaction and the significance for cystic fibrosis are not known. Possible interactions between CFTR and other ion
channels were studied in C127 mouse mammary epithelial cell lines stably transfected with CFTR, ΔF508-CFTR, or vector. Cell
lines were compared quantitatively using an 125I efflux assay and qualitatively using whole-cell patch-clamp recording. As expected, 125I efflux was significantly increased by forskolin only in the CFTR line, and forskolin-stimulated whole-cell currents were
time- and voltage independent. All three lines responded to hypotonic challenge with large 125I efflux responses of equivalent magnitude, and whole-cell currents were outwardly rectified and inactivated at positive voltages.
Unexpectedly, basal 125I efflux was significantly smaller in the ΔF508-CFTR cell line than in either the CFTR or control cell lines (P < 0.0001), and the magnitude of the efflux response to ionomycin was largest in the vector cell line and smallest in the
cell line expressing ΔF508-CFTR (P < 0.01). Whole-cell responses to ionomycin had a linear instantaneous I-V relation and activated at depolarizing voltages. Forskolin responses showed simple summation with responses to ionomycin
or hypotonic challenge. Thus, we found no evidence for interactions between CFTR and the channels responsible for swelling-mediated
responses. Differences were found in basal and ionomycin-stimulated efflux, but these may arise from variations in the clonally
selected cell lines that are unrelated to CFTR expression.
Received: 15 November 1995/Revised: 16 February 1996 相似文献
65.
氟化物对桑蚕幼虫中肠ATP酶活性影响的电镜细胞化学定位 总被引:2,自引:1,他引:1
氟化物对桑蚕幼虫中肠ATP酶活性影响的电镜细胞化学定位陈玉银,包焕盛,吴玉澄(浙江农业大学杭州3100N)桑蚕Bombyxmori(L.)氟中毒导致蚕茧严重减产是目前蚕业界最为关切的问题,有关桑蚕幼虫氟中毒的机理研究也从各方面展开[11]。已知桑蚕氟... 相似文献
66.
67.
从嗜热蓝藻优雅粘囊藻(Myxosarcina concinna Printz.)中分离到具有放氧活性的藻胆体-类囊体膜复合物。它的吸收峰位于680、628、490、438和420 nm ,在低离子强度(0.1~0.4 m ol/L)磷酸缓冲液中的664 nm 荧光发射峰随离子强度的降低而升高,718 nm 荧光发射峰与此相反(77 。K,Ex= 580 nm )。当把游离的藻胆体和已解离去藻胆体的类囊体膜在蔗糖磷酸缓冲液中重组时,随时间延长(0~60 m in),718 nm 荧光发射峰逐渐升高,685 nm 荧光发射峰逐渐下降(77 。K,Ex = 580 nm ),表明藻胆体与类囊体之间的解离和结合对光能传递的影响 相似文献
68.
M. Kato H. J. Bao C.-K. Kang T. Fukui Y. Doi 《Applied microbiology and biotechnology》1996,45(3):363-370
Pseudomonas sp. 61-3 (isolated from soil) produced a polyester consisting of 3-hydroxybutyric acid (3HB) and of medium-chain-length 3-hydroxyalkanoic
acids (3HA) of C6, C8, C10 and C12, when sugars of glucose, fructose and mannose were fed as the sole carbon source. The polyester produced was a blend of homopolymer
and copolymer, which could be fractionated with boiling acetone. The acetone-insoluble fraction of the polyester was a homopolymer
of 3-hydroxybutyrate units [poly (3HB)], while the acetone-soluble fraction was a copolymer [poly(3HB-co-3HA)] containing both short- and medium-chain-length 3-hydroxyalkanoate units ranging from C4 to C12:44 mol% 3-hydroxybutyrate, 5 mol% 3-hydroxyhexanoate, 21 mol% 3-hydroxyoctanoate, 25 mol% 3-hydroxydecanoate, 2 mol% 3-hydroxydodecanoate
and 3 mol% 3-hydroxy-5-cis-dodecenoate. The copolyester was shown to be a random copolymer of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoate
units by analysis of the 13C-NMR spectrum. The poly(3HB) homopolymer and poly (3HB-co-3HA) copolymer were produced simultaneously within cells from glucose in the absence of any nitrogen source, which suggests
that Pseudomonas sp. 61-3 has two types of polyhydroxy-alkanoate syntheses with different substrate specificities.
Received: 9 June 1995/Received last revision: 30 October 1995/Accepted: 6 November 1995 相似文献
69.
生物复苏——大绝灭后生物演化历史的第一幕 总被引:12,自引:1,他引:11
生命史是一部生物界短期,快速剧变与长期,慢速稳定相互交替的历史。大绝灭(即集群绝灭)事件反映了全球环境的大突变,点断了地质历史中的生命记录及其发展历程,预示着生物界的演化出现了最有意义的飞跃,近年来尝试研究大绝灭后全球生物界的残存-复苏及其基本型式,并探索复苏的控制因素,标志着地质科学中一个重心的转移(即从大绝灭转向其后的生物残存与复苏的研究)。生物复苏揭示了大绝灭后生物演化历史的第一幕,其研究的 相似文献
70.
Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase. 总被引:10,自引:0,他引:10
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M Walfridsson X Bao M Anderlund G Lilius L Bülow B Hahn-Hgerdal 《Applied microbiology》1996,62(12):4648-4651
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation. 相似文献