排序方式: 共有113条查询结果,搜索用时 203 毫秒
51.
Abdolreza Daneshvar Amoli Nazanin Mohebali Parvaneh Farzaneh Seyed Abolhassan Shahzadeh Fazeli Laleh Nikfarjam Sepideh Ashouri Movasagh Zahra Moradmand Meysam Ganjibakhsh Ahmad Nasimian Mehrnaz Izadpanah Faezeh Vakhshiteh Neda sadat Gohari Najmeh sadat Masoudi Maryam Farghadan Shiva Mohamadi Moghanjoghi Masoud Khalili Kourosh J. Khaledi 《In vitro cellular & developmental biology. Animal》2017,53(4):337-343
Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genetic resources. It will also create an accessible database for researchers. 相似文献
52.
53.
Seyedeh Masoumeh Ghoreishi Mehrangiz Amiri Ali Shabestani Monfared Faezeh Hamidi Hossein Najafzadehvarzi 《Saudi Journal of Biological Sciences》2022,29(8):103353
Due to the growth of diabetic mellitus (DM) and diabetic nephropathy as a significant complication for diabetic patients, study on effective treatment with fewer side effects has been fascinated. In this study for the first time carvedilol effects on both function and structure of kidney in diabetic nephropathy treatment were evaluated. Diabetes was induced by injection of streptozotocin (STZ) intravenously in rats and three groups including control, diabetic, and treatment with carvedilol were considered. Biochemical parameters such as, blood glucose level, BUN, creatinine, uric acid, Na+, K+ was determined. Results showed that glucose (516 to 291 mg/dl), BUN (42 to 21.67 mg/dl), creatinine (0.75 to 0.6 mg/dl), uric acid (4.45 to 1.36 mg/dl), and K+ (7.433 to 5.433 mEq/l) level reduced. Decrease in glucose, BUN, creatinine, uric acid, and K+ and increase in Na+ level (138 to 146.33 mEq/l) confirmed therapeutic effect of carvedilol. Furthermore, the histopathological study was done for each group. Histopathological results confirmed the data obtained by biochemical parameters. For further investigation, SPECT imaging with 99mTc-DMSA, which is a gold standard in diabetic nephropathy detection, was done. SPECT imaging showed that accumulation of 99mTc-DMSA was increased in treated group (5 to 25 kcpm) which means the improvement in renal structure in the treated group compare to the diabetic group (5 kcpm). Finally, obtained results confirmed our hypothesis that carvedilol had a therapeutic effect on diabetic nephropathy. 相似文献
54.
55.
56.
Samira Basharkhah Faezeh Sabet Sanaz Ahmadi Ghezeldasht Arman Mosavat Hamid Reza Jahantigh Elham Barati Khosrow Shamsian Masoud Saleh‐Moghaddam Hiva Sharebyani Tahereh Hassannia Seyed Ali Akbar Shamsian 《Microbiology and immunology》2019,63(11):449-457
Hepatitis C virus (HCV) infection is a major public health problem with about 1.75 million new HCV cases and 71 million chronic HCV infections worldwide. The study aimed to evaluate clinical, serological, molecular, and liver markers to develop a mathematical predictive model for the quantification of the HCV viral load in chronic HCV infected patients. In this cross‐sectional study, blood samples were taken from 249 recently diagnosed HCV‐infected subjects and were tested for liver condition, viral genotype, and HCV RNA load. Receiver operating characteristics (ROC) curves and multiple linear regression analysis were used to predict the HCV‐RNA load. Genotype 3 followed by genotype 1 were the most prevalent genotypes in Mashhad, Northeastern Iran. The maximum levels of viral load were detected in the mixed genotype group, and the lowest levels in the undetectable genotype group. The log of the HCV viral load was significantly associated with thrombocytopenia and higher serum levels of alanine transaminase (ALT). In addition, the log HCV RNA was significantly higher in patients with arthralgia, fatigue, fever, vomiting, or dizziness. Moreover, genotype 3 was significantly associated with icterus. A ROC curve analysis revealed that the best cut‐off points for serum levels of aspartate aminotransferase (AST), ALT, and alkaline phosphatase (ALP) were >31, >34, and ≤246 IU/L, respectively. Sensitivity, specificity, and positive predictive values for AST were 87.7%, 84.36%, and 44.6%, for ALT they were 83.51%, 81.11%, and 36%, and for ALP were 72.06%, 42.81%, and 8.3%, respectively. A mathematical regression model was developed that could estimate the HCV‐RNA load. Regression model: log viral load = 7.69 ? 1.01 × G3 ? 0.7 × G1 + 0.002 × ALT ? 0.86 × fatigue. 相似文献
57.
In order to investigate the capability of two chelators deferasirox (DFX or ICL670) and deferiprone (L1) in removing lead from the body, the present research was performed. Two does levels of 40 and 80 mg/kg body weight of lead (II) chloride was given to rats as biological model for 45 days. After 45 days, some toxicity symptoms were observed in rats such as loss of hair and weight, appearance of red dots around eyes, weakness and irritability. After lead application, chelation therapy with DFX and L1 as mono and combined (DFX, L1 and DFX + L1) was done for 10 days. After chelation therapy, lead level in different tissues reduced. The combined chelation therapy results showed that these chelators are able to remove lead from the body and toxicity symptoms decreased. The combined therapy results (DFX + L1) show higher efficacy and lower toxicity compared to single therapies. 相似文献
58.
Dadkhah A Fatemi F Kazemnejad S Rasmi Y Ashrafi-Helan J Allameh A 《Molecular and cellular biochemistry》2006,281(1-2):145-152
Recently we reported that ferric reducing ability of plasma (FRAP) assay, as an index of total antioxidant activity, increases
in growing rats in response to high dose of vitamin K. In this study, it was found that acetaminophen (APAP) can cause elevation
in FRAP in suckling and adult rats. This study was initiated to assess the contribution of individual antioxidant factors
on elevation in FRAP. A surge in FRAP, 1 h after high dose APAP (250 or 450 mg/kg BW) administration was recorded in both
young as well as adults. Whereas, low dose drug (25 mg/kg) failed to alter FRAP in both the age groups. Time-course studies
show that drug-dependent elevation in FRAP begin rapidly, reaching a maximum at 1 h (> 500%). Increased FRAP was associated
with a marked increase (∼14-fold) in plasma bilirubin, 6 h after drug administration at 450 mg/kg only in suckling rats. Similarly,
APAP-related increase in superoxide dismutase activity in erythrocytes was limited to young rats of both the age groups. Other
factors measured during this period viz., plasma uric acid, bilirubin and total protein together with catalase activity of
erythrocytes remained unchanged in treated rats. Under these circumstances, APAP-related depletion in liver glutathione was
almost similar in both the age groups. During a 12 h study, the concentration of lipid peroxidation products, in liver of
treated groups remained within the levels of respective controls. The endpoint hepatotoxic effects of APAP was almost similar
in both the age groups, suggesting that like adults, immature rats can cope with toxic effects of APAP owing to their drug-dependent
induction in certain antioxidant factors. 相似文献
59.
Fatemeh Hosseininaveh Parvin Razavi Tabatabaei Ali Darvishzadeh Faezeh Bagheri 《Archives Of Phytopathology And Plant Protection》2013,46(14):1704-1715
Pistachio fruit hull borer, Arimania komaroffi Ragonot (Lep.: Pyralidae), is one the most important pests of pistachio in Iran. The larvae spin web as well as bore into young fruits, and the infested fruits fall off the trees. The second-generation adult moths appear in August and September, and their offspring feed on the fruit hull. Results indicated the presence of α-amylase, α-glucosidase, β-glucosidase, α-galactosidase, β-galactosidase and some proteases in the digestive tract of the pest. Highest activities of α-amylase, α-glucosidase, β-glucosidase, α-galactosidase and β-galactosidase were at pH 10, 7, 7, 6 and pH 6, respectively. Highest activities of trypsin, chymotrypsin and elastase of larval midgut were at pH 11. Zymogram analysis of α-amylase, α-glucosidase, β-glucosidase, tryptic, chymotryptic and elastase using native-PAGE revealed 1, 1, 2, 3, 3 and 2 bands of activity respectively, in A. komaroffi. One band was disappeared in the presence of the inhibitor TLCK, but no further inhibition by the inhibitors TPCK was observed. The results can be of help for designing new strategies for controlling the pistachio fruit hull borer based on natural proteases and carbohydrase inhibitors. 相似文献
60.