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排序方式: 共有199条查询结果,搜索用时 31 毫秒
81.
Function of the conserved FHIPEP domain of the flagellar type III export apparatus,protein FlhA
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Clive S. Barker Tomoharu Inoue Irina V. Meshcheryakova Seiya Kitanobo Fadel A. Samatey 《Molecular microbiology》2016,100(2):278-288
The Type III flagellar protein export apparatus of bacteria consists of five or six membrane proteins, notably FlhA, which controls the export of other proteins and is homologous to the large family of FHIPEP export proteins. FHIPEP proteins contain a highly‐conserved cytoplasmic domain. We mutagenized the cloned Salmonella flhA gene for the 692 amino acid FlhA, changing a single, conserved amino acid in the 68‐amino acid FHIPEP region. Fifty‐two mutations at 30 positions mostly led to loss of motility and total disappearance of microscopically visible flagella, also Western blot protein/protein hybridization showed no detectable export of hook protein and flagellin. There were two exceptions: a D199A mutant strain, which produced short‐stubby flagella; and a V151L mutant strain, which did not produce flagella and excreted mainly un‐polymerized hook protein. The V151L mutant strain also exported a reduced amount of hook‐cap protein FlgD, but when grown with exogenous FlgD it produced polyhooks and polyhook‐filaments. A suppressor mutant in the cytoplasmic domain of the export apparatus membrane protein FlhB rescued export of hook‐length control protein FliK and facilitated growth of full‐length flagella. These results suggested that the FHIPEP region is part of the gate regulating substrate entry into the export apparatus pore. 相似文献
82.
Fadel Mansour 《BioControl》1988,33(3):371-375
One primary parasite,Trioxys pallidus (Haliday) (Hym.: Braconidae), and a secondary parasite,Aphidencyrtus sp., were identified from the blackmargined aphid,Monellia caryella (Fitch) (Hom.: Aphididae), in Israeli pecan orchards. The average total parasitism for all locations sampled was 13.5%. Although a hyperparasite was
discovered, it had no significantly detrimental effect on the parasite.
Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, No. 2034-E, 1987 series. 相似文献
83.
Safaa S. Hassan Nashwa El-Khazragy Amal A. Elshimy Marwa M. Aboelhussein Shereen A. Saleh Sayed Fadel Hend A. Atia Safa Matbouly Natalie Tamer 《Journal of cellular biochemistry》2020,121(4):2811-2817
Hepatitis C virus (HCV) infection is a major public health problem, having a high prevalence in Egypt. Leukemia and lymphoma have been associated with HCV infection. MicroRNA-155 (miR-155) has been reported to play a regulatory role in cancer, inflammation, and immune response to infection. The expression level of miR-155 in HCV viremic patients is controversial; although high miR-155 levels were demonstrated in HCV genotypes 1,2, and 3, low levels of miR-155 were detected in Egyptian patients with HCV genotype 4. Several studies have investigated the correlation between the levels of miRNA-155 and the replication of HCV, others have evaluated miRNA-155 as a prognostic biomarker in different types of cancer. No studies have investigated the impact of miRNA-155 knockdown on HCV pediatric patients associated with childhood acute lymphoblastic leukemia (ALL). We knocked-out the miR_155a in cultured polymorphonuclear cells (PBMCs) obtained from 60 children with ALL; 30 were associated with HCV-4 infection and 30 were HCV negative. The miR_155a, HCV viral load, and cell proliferation werre assessed in treated and untreated cells using TaqMan assay quantitative polymerase chain reaction. We found that miRNA-155 was significantly upregulated by seven folds in the HCV-4 associated ALL group; while being linked to high HCV viral load and leukemic burden, miR_155a knock-out can improve the disease outcome. We conclude that miR-155 is a critical miRNA that is considered a therapeutic target in pediatric HCV leukemic patients. 相似文献
84.
The direct effects of ethanol and caffeine on embryogenesis were investigated using the whole rat embryo culture system. Compared to control embryos, the crown-rump length, number of somites, branchial bars, and morphological score were significantly reduced in embryos exposed to ethanol, caffeine, or both substances. Development of the craniofacial region, cardiac primordium and forelimb was delayed following ethanol treatment. Compared to the controls, the anterior neuropore lagged in development following caffeine treatment; closure of the posterior neuropore was significantly delayed in each of the treatment groups. The optic and olfactory primordia were not affected. The results indicate that alcohol and caffeine independently affect the embryo, but when combined their effects were not potentiated. 相似文献
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C André Lévesque Henk Brouwer Liliana Cano John P Hamilton Carson Holt Edgar Huitema Sylvain Raffaele Gregg P Robideau Marco Thines Joe Win Marcelo M Zerillo Gordon W Beakes Jeffrey L Boore Dana Busam Bernard Dumas Steve Ferriera Susan I Fuerstenberg Claire MM Gachon Elodie Gaulin Francine Govers Laura Grenville-Briggs Neil Horner Jessica Hostetler Rays HY Jiang Justin Johnson Theerapong Krajaejun Haining Lin Harold JG Meijer Barry Moore Paul Morris Vipaporn Phuntmart Daniela Puiu Jyoti Shetty Jason E Stajich Sucheta Tripathy Stephan Wawra Pieter van West Brett R Whitty Pedro M Coutinho Bernard Henrissat Frank Martin Paul D Thomas Brett M Tyler Ronald P De Vries Sophien Kamoun Mark Yandell Ned Tisserat C Robin Buell 《Genome biology》2010,11(7):1-22
88.
Avendaño-Reyes L Hernández-Rivera JA Alvarez-Valenzuela FD Macías-Cruz U Díaz-Molina R Correa-Calderón A Robinson PH Fadel JG 《International journal of biometeorology》2012,56(6):993-999
Heat stress generates a significant economic impact for the dairy industry in arid and semi-arid regions of the world, so that heat abatement is an important issue for dairy producers. The objective of this study was to evaluate effects of two short-term cooling periods on physiological and productive status of lactating Holstein cows during hot ambient temperatures. Thirty-nine multiparous cows were blocked by milk yield and assigned to one of three treatments including: control group (C), cows cooled before milking time (0500 and 1700 h daily, 1 h cooling); AM group, cows cooled at 1000 h and before milking (2 h cooling); and AM?+?PM group, cows cooled at 1100, 1500 and 2200 h, as well as before milking (4 h cooling). The cooling system was placed in the holding pen which the cows were moved through for cooling. Respiratory rate, and temperatures of thurl and right flank, were lower (P?0.05) in cows from the AM?+?PM group than AM and C cows during the morning and afternoon. However, udder temperature was higher in the AM?+?PM group compared to AM and C groups during the afternoon, although lower than the AM group during the morning. Rectal temperature was similar in all groups. Thyroxin concentrations tended (P?0.10) to be lower in AM?+?PM relative to the AM and C groups. The AM?+?PM group had higher (P?0.05) milk production than C (18.70 vs. 17.43 kg, respectively), and AM?+?PM cows had a trend (P?0.10) to increased milk energy output vs. the C and AM groups (13.75 vs. 13.18 and 13.15 Mcal, respectively). Protein and fat in milk, body condition score, glucose, cholesterol, triglycerides and triiodothyronine were similar among the groups. Four hours of cooling with spray and fans during severe summer temperatures only modestly improved milk yield of lactating Holstein cows. 相似文献
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