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961.
962.
Gemma N. Villagomez Fabian Nürnberger Fabrice Requier Susanne Schiele Ingolf SteffanDewenter 《Ecology and evolution》2021,11(12):7834
Temperature and photoperiod are important Zeitgebers for plants and pollinators to synchronize growth and reproduction with suitable environmental conditions and their mutualistic interaction partners. Global warming can disturb this temporal synchronization since interacting species may respond differently to new combinations of photoperiod and temperature under future climates, but experimental studies on the potential phenological responses of plants and pollinators are lacking. We simulated current and future combinations of temperature and photoperiod to assess effects on the overwintering and spring phenology of an early flowering plant species (Crocus sieberi) and the Western honey bee (Apis mellifera). We could show that increased mean temperatures in winter and early spring advanced the flowering phenology of C. sieberi and intensified brood rearing activity of A. mellifera but did not advance their brood rearing activity. Flowering phenology of C. sieberi also relied on photoperiod, while brood rearing activity of A. mellifera did not. The results confirm that increases in temperature can induce changes in phenological responses and suggest that photoperiod can also play a critical role in these responses, with currently unknown consequences for real‐world ecosystems in a warming climate. 相似文献
963.
964.
Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input 总被引:2,自引:0,他引:2
Udiković-Kolić N Devers-Lamrani M Petrić I Hršak D Martin-Laurent F 《Applied microbiology and biotechnology》2011,90(4):1547-1554
We evaluated the effects of variations in atrazine input on the evolution of a bacterial culture adapted to a low atrazine
concentration. This initial culture (M3-K) was subjected to weekly subculturing in the presence of a high concentration of
atrazine as the only N source (100 mg l−1). After four subculturing, M3-K evolved to a new bacterial culture (M3) which exhibited a significant increase in the extent
of atrazine mineralization in comparison with the initial culture. Molecular analyses of M3-K and M3 cultures by cloning,
restriction analysis, and sequencing of the 16S rRNA genes revealed significant differences in culture structure and composition.
M3-K culture comprised mainly Actinobacteria (40%), β-Proteobacteria (26%), and Bacteroidetes (16%). After exposure to a high atrazine concentration, the dominance of Actinobacteria decreased (14%), Bacteroidetes increased (27%), and β-Proteobacteria were replaced by γ-Proteobacteria (32%). Quantitative PCR revealed that the abundance of atzB and atzC genes relative to total bacteria decreased by a factor of 3–4 following the increase in atrazine concentration, while the
relative abundance of trzD increased significantly (≈400 times). Presented study shows that variations in atrazine input drive both functional and compositional
shifts in the atrazine-degrading bacterial culture. 相似文献
965.
Levaot N Voytyuk O Dimitriou I Sircoulomb F Chandrakumar A Deckert M Krzyzanowski PM Scotter A Gu S Janmohamed S Cong F Simoncic PD Ueki Y La Rose J Rottapel R 《Cell》2011,147(6):1324-1339
Cherubism is an autosomal-dominant syndrome characterized by inflammatory destructive bony lesions resulting in symmetrical deformities of the facial bones. Cherubism is caused by mutations in Sh3bp2, the gene that encodes the adaptor protein 3BP2. Most identified mutations in 3BP2 lie within the peptide sequence RSPPDG. A mouse model of cherubism develops hyperactive bone-remodeling osteoclasts and systemic inflammation characterized by expansion of the myelomonocytic lineage. The mechanism by which cherubism mutations alter 3BP2 function has remained obscure. Here we show that Tankyrase, a member of the poly(ADP-ribose)polymerase (PARP) family, regulates 3BP2 stability through ADP-ribosylation and subsequent ubiquitylation by the E3-ubiquitin ligase RNF146 in osteoclasts. Cherubism mutations uncouple 3BP2 from Tankyrase-mediated protein destruction, which results in its stabilization and subsequent hyperactivation of the SRC, SYK, and VAV signaling pathways. 相似文献
966.
Ganeff C Remouchamps C Boutaffala L Benezech C Galopin G Vandepaer S Bouillenne F Ormenese S Chariot A Schneider P Caamaño J Piette J Dejardin E 《Molecular and cellular biology》2011,31(21):4319-4334
Several tumor necrosis factor receptor (TNFR) family members activate both the classical and the alternative NF-κB pathways. However, how a single receptor engages these two distinct pathways is still poorly understood. Using lymphotoxin β receptor (LTβR) as a prototype, we showed that activation of the alternative, but not the classical, NF-κB pathway relied on internalization of the receptor. Further molecular analyses revealed a specific cytosolic region of LTβR essential for its internalization, TRAF3 recruitment, and p100 processing. Interestingly, we found that dynamin-dependent, but clathrin-independent, internalization of LTβR appeared to be required for the activation of the alternative, but not the classical, NF-κB pathway. In vivo, ligand-induced internalization of LTβR in mesenteric lymph node stromal cells correlated with induction of alternative NF-κB target genes. Thus, our data shed light on LTβR cellular trafficking as a process required for specific biological functions of NF-κB. 相似文献
967.
Secondary succession on calcareous hillsides, initiated following the abandonment of agro-pastoral practices, is characterized by the transformation of initial short sward into tall herbaceous vegetation and finally woody formations. These structural changes are accompanied by modifications in ecological conditions but some species are able to persist and flower along the successional gradient. In this study, we compare reproductive and morphological traits of seven perennial species present in three successional stages [short grassland (SG), tall grassland (TG), and encroached grassland (EG)] to test if plant species present modifications along the successional gradient. The results show that morphological traits as height, leaf area and leaf dry mass increase for all studied species. Along the successional gradient, Teucrium chamaedrys increases ramet biomass more than twice (+145%) while Brachypodium pinnatum increases it even more (+340%). Maximum specific leaf area was observed in Brachypodium pinnatum (SLA = 20.4 mm2 mg−1) in SG, whereas Helianthemum nummularium and Teucrium chamaedrys have both rising SLA and falling leaf dry matter content in TG and EG. Concurrently, Helianthemum nummularium, Hippocrepis comosa and Teucrium chamaedrys show a clear decrease in sexual reproduction, with between 18 and 40% fewer flowers during the progress of succession. By contrast, Festuca lemanii, Sesleria caerulea and Brachypodium pinnatum increase their flower numbers per inflorescence (×3.6, ×3.3, and ×3.5, respectively). Festuca lemanii and Sesleria caerulea increase seed production to a maximum in TG, and Brachypodium pinnatum and Carex flacca increase seed output in TG and EG (×4.5, ×5.5, respectively). Changes in plants traits underscore the idea that species allocate resources differently probably because of increasing competition and decreasing edaphic constraints. This study also implies the potential existence of specific responses allowing plants to maintain existence within a plant community and explaining their contrasting performance during the succession. 相似文献
968.
Arthropods and nematodes are important protagonists in human health because either they act as vectors of pathogens (bacteria, protozoa, viruses or fungus), or are themselves parasites. Fighting infectious diseases is based essentially on vaccination (prevention) or chemotherapeutic (curative) approaches in human, but one can envisage as an alternative to reduce the number of vectors or limit their ability to spread pathogens. Such strategies controlling dissemination will undoubtedly benefit from the knowledge accumulated by recent works on powerful mechanisms developed by symbiotic insect bacteria such as Wolbachia to popagate in arthropods and nematods. This review summarizes these recent data, and indicate how these mechanisms can be manipulated to reduce the dissemination of insect vectors propagating human diseases. 相似文献
969.
Herraiz C Journé F Abdel-Malek Z Ghanem G Jiménez-Cervantes C García-Borrón JC 《Molecular endocrinology (Baltimore, Md.)》2011,25(1):138-156
Melanocortin 1 receptor (MC1R), a Gs protein-coupled receptor expressed in melanocytes, is a major determinant of skin pigmentation, phototype and cancer risk. Upon stimulation by αMSH, MC1R triggers the cAMP and ERK1/ERK2 MAPK pathways. In mouse melanocytes, ERK activation by αMSH binding to Mc1r depends on cAMP, and melanocytes are considered a paradigm for cAMP-dependent ERK activation. However, human MC1R variants associated with red hair, fair skin [red hair color (RHC) phenotype], and increased skin cancer risk display reduced cAMP signaling but activate ERKs as efficiently as wild type in heterologous cells, suggesting independent signaling to ERKs and cAMP in human melanocytes. We show that MC1R signaling activated the ERK pathway in normal human melanocytes and melanoma cells expressing physiological levels of endogenous RHC variants. ERK activation was comparable for wild-type and mutant MC1R and was independent on cAMP because it was neither triggered by stimulation of cAMP synthesis with forskolin nor blocked by the adenylyl cyclase inhibitor 2',5'-dideoxyadenosine. Stimulation of MC1R with αMSH did not lead to protein kinase C activation and ERK activation was unaffected by protein kinase C inhibitors. Conversely, pharmacological interference, small interfering RNA studies, expression profiles, and functional reconstitution experiments showed that αMSH-induced ERK activation resulted from Src tyrosine kinase-mediated transactivation of the stem cell factor receptor, a receptor tyrosine kinase essential for proliferation, differentiation, and survival of melanocyte precursors, thus demonstrating a functional link between the stem cell factor receptor and MC1R. Moreover, this transactivation phenomenon is unique because it is unaffected by natural mutations impairing canonical MC1R signaling through the cAMP pathway. 相似文献
970.
Isabelle Rauly-Lestienne Fabrice LestienneMarie-Christine Ailhaud Johan BinesseAdrian Newman-Tancredi Didier Cussac 《Cellular signalling》2011,23(1):58-64
Following agonist action, G-protein-coupled receptors may exhibit differential coupling to G-proteins or second messenger pathways, supporting the notion of agonist-directed trafficking. To explore these mechanisms, we have designed and transfected synthetic siRNA duplexes to knockdown different Gα subunits in Chinese hamster ovary (CHO) cells expressing human (h)5-hydroxytryptamine 1A receptors (CHO-h5-HT1A). siRNAs against Gαi2 and Gαi3 transfected alone or in combination caused a large decrease in the corresponding mRNA level (64-80%) and also at the protein level for Gαi3 (60-70%), whereas a non-specific siRNA showed no effect. In membranes of CHO-h5-HT1A, 5-HT stimulated guanosine-5′-O-(3-[35S]thio)-triphosphate ([35S]GTPγS) binding was differentially affected by transfection of siRNAs against Gαi protein, siRNAs against Gαi2 inducing a more important decrease in the efficacy of 5-HT than transfection of siRNAs against Gαi3. The high potency component was abolished after transfection of siRNAs against Gαi3 and the lower potency component was suppressed after transfection of siRNAs against Gαi2. To directly investigate Gαi3 activation we used an antibody-capture/scintillation proximity assay. (+)8-OH-DPAT yielded bell-shaped curves for Gαi3 activation, a response that was abolished after transfection of siRNAs against Gαi3 protein. Interestingly, (+)8-OH-DPAT yielded a sigmoidal response when only Gαi3 protein was expressed. These data suggest that when efficacious agonists attain a high level of occupation of h5-HT1A receptors, a change occurs that induces coupling to Gαi2 protein and suppresses signalling through Gαi3 subunits. 相似文献