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排序方式: 共有1201条查询结果,搜索用时 16 毫秒
991.
Elsa Hadj Bachir Charles Poiraud Sonia Paget Nicolas Stoup Soumaya El Moghrabi Belinda Duchêne Nathalie Jouy Antonino Bongiovanni Meryem Tardivel Louis-Bastien Weiswald Marie Vandepeutte Csar Beugniez Fabienne Escande Emmanuelle Leteurtre Laurent Poulain Chann Lagadec Pascal Pigny Nicolas Jonckheere Florence Renaud Stephanie Truant Isabelle Van Seuningen Audrey Vincent 《Biology of the cell / under the auspices of the European Cell Biology Organization》2022,114(1):32-55
992.
Sarah A. C. Falc?o Tiffany Weinkopff Benjamin P. Hurrell Fabiana S. Celes Rebecca P. Curvelo Deboraci B. Prates Aldina Barral Valeria M. Borges Fabienne Tacchini-Cottier Camila I. de Oliveira 《PLoS neglected tropical diseases》2015,9(3)
BackgroundNeutrophils are the first line of defense against invading pathogens and are rapidly recruited to the sites of Leishmania inoculation. During Leishmania braziliensis infection, depletion of inflammatory cells significantly increases the parasite load whereas co-inoculation of neutrophils plus L. braziliensis had an opposite effect. Moreover, the co-culture of infected macrophages and neutrophils also induced parasite killing leading us to ask how neutrophils alone respond to an L. braziliensis exposure. Herein we focused on understanding the interaction between neutrophils and L. braziliensis, exploring cell activation and apoptotic fate.ConclusionsWe show that L. braziliensis induces neutrophil recruitment in vivo and that neutrophils exposed to the parasite in vitro respond through activation and release of inflammatory mediators. This outcome may impact on parasite elimination, particularly at the early stages of infection. 相似文献
993.
Stéphane Maillard Francesca Damiola Enora Clero Maroulio Pertesi Nivonirina Robinot Frédérique Rachédi Jean-Louis Boissin Joseph Sebbag Larrys Shan Frédérique Bost-Bezeaud Patrick Petitdidier Fran?oise Doyon Constance Xhaard Carole Rubino Hélène Blanché Vladimir Drozdovitch Fabienne Lesueur Florent de Vathaire 《PloS one》2015,10(4)
Background
French Polynesia has one of the highest incidence rates of thyroid cancer worldwide. Relationships with the atmospheric nuclear weapons tests and other environmental, biological, or behavioral factors have already been reported, but genetic susceptibility has yet to be investigated. We assessed the contribution of polymorphisms at the 9q22.33 and 14q13.3 loci identified by GWAS, and within the DNA repair gene ATM, to the risk of differentiated thyroid cancer (DTC) in 177 cases and 275 matched controls from the native population.Principal Findings
For the GWAS SNP rs965513 near FOXE1, an association was found between genotypes G/A and A/A, and risk of DTC. A multiplicative effect of allele A was even noted. An excess risk was also observed in individuals carrying two long alleles of the poly-alanine tract expansion in FOXE1, while no association was observed with rs1867277 falling in the promoter region of the gene. In contrast, the GWAS SNP rs944289 (NKX2-1) did not show any significant association. Although the missense substitution D1853N (rs1801516) in ATM was rare in the population, carriers of the minor allele (A) also showed an excess risk. The relationships between these five polymorphisms and the risk of DTC were not contingent on the body surface area, body mass index, ethnicity or dietary iodine intake. However, an interaction was evidenced between the thyroid radiation dose and rs944289.Significance
A clear link could not be established between the high incidence in French Polynesia and the studied polymorphisms, involved in susceptibility to DTC in other populations. Important variation in allele frequencies was observed in the Polynesian population as compared to the European populations. For FOXE1 rs965513, the direction of association and the effect size was similar to that observed in other populations, whereas for ATM rs1801516, the minor allele was associated to an increased risk in the Polynesian population and with a decreased risk in the European population. 相似文献994.
Giulia Galotto Pattipong Wisanpitayakorn Jeffrey P Bibeau Yen-Chun Liu Fabienne Furt Ellen C Pierce Parker J Simpson Erkan Tüzel Luis Vidali 《Plant physiology》2021,187(4):2509
In tip-growing plant cells, growth results from myosin XI and F-actin-mediated deposition of cell wall polysaccharides contained in secretory vesicles. Previous evidence showed that myosin XI anticipates F-actin accumulation at the cell’s tip, suggesting a mechanism where vesicle clustering via myosin XI increases F-actin polymerization. To evaluate this model, we used a conditional loss-of-function strategy by generating moss (Physcomitrium patens) plants harboring a myosin XI temperature-sensitive allele. We found that loss of myosin XI function alters tip cell morphology, vacuolar homeostasis, and cell viability but not following F-actin depolymerization. Importantly, our conditional loss-of-function analysis shows that myosin XI focuses and directs vesicles at the tip of the cell, which induces formin-dependent F-actin polymerization, increasing F-actin’s local concentration. Our findings support the role of myosin XI in vesicle focusing, possibly via clustering and F-actin organization, necessary for tip growth, and deepen our understanding of additional myosin XI functions.Vesicle clustering by the molecular motor myosin XI enhances actin polymerization-dependent motility and polarized vesicle accumulation in tip-growing cells. 相似文献
995.
Fabienne Clot Carmen Gianfrani Marie-Claude Babron Faouzi Bouguerra Scott Southwood Martin F. Kagnoff Riccardo Troncone Selvaggia Percopo Jean-François Eliaou Françoise Clerget-Darpoux Alessandro Sette L. Greco 《Immunogenetics》1999,49(9):800-807
Celiac disease (CD) patients usually express a DQ2 heterodimer, whose chains DQα1*0501/DQβ1*0201, are encoded by the genes
HLA-DQA1*0501 and DQB1*0201, respectively. Among the DQ2 carriers, the risk of developing disease was shown to correlate with the number of DQβ1*0201
chains encoded. Studying two separate cohorts of Italian and Tunisian patients, we now show a significant association of celiac
disease with expression of either the DQ2 or DR53 heterodimers. The risk is maximal for individuals that carry both DQ2 and
DR53 heterodimers. When twenty synthetic peptides overlapping most of A-gliadin sequence were tested for the binding to various
purified DR molecules, it was found that DR53 molecules bind selectively and with high affinity (IC50<1 μM) to A-gliadin-derived
peptides. These data suggest that both HLA DQ2 and DR53 molecules are associated with increased genetic risk for CD, and provide
a possible biochemical basis for this complex association.
Received: 1 August 1998 / Revised: 24 February 1999 相似文献
996.
997.
998.
Paul Jacobs Marc Massaer Michel Heinderyckx Fabienne Milican Pascal Gilles Omer van Opstal Pierre Voet Dirck Gheysen Alex Bollen 《Molecular biology reports》1991,15(2):73-79
The DNA coding for the circumsporozoite protein (CPS) of Plasmodium falciparum has been cloned into the baculovirus expression vector pAcYM1 and expressed in Spodoptera frugiperda (Sf9) insect cells. Three DNA constructs have been made: the first one directs the synthesis of the complete CSP (aa 1–412), the second leads to the production of a species devoid of the anchor domain (aa 1–391) and the third one to a molecule lacking both signal and membrane anchor sequences (aa 18–391). All three recombinant CPS were produced at about 3 g per 106 infected cells and were characterized in terms of immunoreactivity and apparent molecular weight. Analytical purification of the recombinant proteins was achieved by a combination of heat treatment, acidification, isoelectric focusing and ion exchange chromatography. The purified material, when injected into mice, generated only modest antibody responses, although antisera from immunized mice reacted with control CSP antigens carrying or not the major immunodominant repeat region.Abbreviations
AcNPV
Autographa californica nuclear polyhedrosis virus
- CSP
circumsporozoite protein 相似文献
999.
1000.
Preliminary results are reported of a molecular dynamics calculation of free energy variations during the dissociation of an antigen-antibody complex, hen egg-white lysozyme — Fab D1.3, using atomic coordinates determined by the group of R. J. Poljak, and explicit handling of solvent molecules. After equilibration of the complex in solution at 300 K, a dissociation path was generated by a directed dynamics protocol. Then the thermodynamic perturbation method was used for computing the derivative of the free energy of the system with respect to dissociation coordinate, both for the undissociated complex and in two points along the path. 200-ps molecular dynamics simulations were carried out at each of these points. The results obtained are discussed, with special emphasis on the role of interstitial water in the appearance of a hydrophobic activation free energy.Université Paul Sabatier, and URA 505 of C.N.R.S. 相似文献