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231.
232.
Marko Jovi Fabien Kieken Naava Naslavsky Paul L. Sorgen Steve Caplan 《Molecular biology of the cell》2009,20(11):2731-2743
The C-terminal Eps15 homology domain (EHD) 1/receptor-mediated endocytosis-1 protein regulates recycling of proteins and lipids from the recycling compartment to the plasma membrane. Recent studies have provided insight into the mode by which EHD1-associated tubular membranes are generated and the mechanisms by which EHD1 functions. Despite these advances, the physiological function of these striking EHD1-associated tubular membranes remains unknown. Nuclear magnetic resonance spectroscopy demonstrated that the Eps15 homology (EH) domain of EHD1 binds to phosphoinositides, including phosphatidylinositol-4-phosphate. Herein, we identify phosphatidylinositol-4-phosphate as an essential component of EHD1-associated tubules in vivo. Indeed, an EHD1 EH domain mutant (K483E) that associates exclusively with punctate membranes displayed decreased binding to phosphatidylinositol-4-phosphate and other phosphoinositides. Moreover, we provide evidence that although the tubular membranes to which EHD1 associates may be stabilized and/or enhanced by EHD1 expression, these membranes are, at least in part, pre-existing structures. Finally, to underscore the function of EHD1-containing tubules in vivo, we used a small interfering RNA (siRNA)/rescue assay. On transfection, wild-type, tubule-associated, siRNA-resistant EHD1 rescued transferrin and β1 integrin recycling defects observed in EHD1-depleted cells, whereas expression of the EHD1 K483E mutant did not. We propose that phosphatidylinositol-4-phosphate is an essential component of EHD1-associated tubules that also contain phosphatidylinositol-(4,5)-bisphosphate and that these structures are required for efficient recycling to the plasma membrane. 相似文献
233.
Perugi F Muriaux D Ramirez BC Chabani S Decroly E Darlix JL Blot V Pique C 《Molecular biology of the cell》2009,20(1):498-508
Human immunodeficiency virus (HIV)-1 replication is positively or negatively regulated through multiple interactions with host cell proteins. We report here that human Discs Large (Dlg1), a scaffold protein recruited beneath the plasma membrane and involved in the assembly of multiprotein complexes, restricts HIV-1 infectivity. The endogenous Dlg1 and HIV-1 Gag polyprotein spontaneously interact in HIV-1-chronically infected T cells. Depleting endogenous Dlg1 in either adherent cells or T cells does not affect Gag maturation, production, or release, but it enhances the infectivity of progeny viruses five- to sixfold. Conversely, overexpression of Dlg1 reduces virus infectivity by ~80%. Higher virus infectivity upon Dlg1 depletion correlates with increased Env content in cells and virions, whereas the amount of virus-associated Gag or genomic RNA remains identical. Dlg1 knockdown is also associated with the redistribution and colocalization of Gag and Env toward CD63 and CD82 positive vesicle-like structures, including structures that seem to still be connected to the plasma membrane. This study identifies both a new negative regulator that targets the very late steps of the HIV-1 life cycle, and an assembly pathway that optimizes HIV-1 infectivity. 相似文献
234.
235.
Mi-Young Song Fabien Leprieur Alain Thomas Sithan Lek-Ang Tae-Soo Chon Sovan Lek 《Aquatic Ecology》2009,43(4):999-1009
The impact of agricultural land use on the composition and structure of aquatic insect assemblages (i.e., taxa of Ephemeroptera,
Plecoptera, Trichoptera, and Coleoptera (EPTC)) was investigated in tributary streams of the Garonne river basin, southern
France. The self-organizing map (SOM) method was applied to compare both instream environmental conditions and EPTC assemblages
between forest and agricultural streams. According to the SOM model, the study sites were classified into three main clusters
corresponding to distinct EPTC assemblages. The SOM cluster associated with most of the agricultural sites had lower EPTC
species richness and diversity. This cluster was also characterized by high levels of total dissolved solids, nitrate (NO3), and chemical oxygen demand. Overall, our study shows that agricultural streams when compared with forest streams had lower
biological integrity. In accordance with the European Water Framework Directive, our results indicate that the sites most
impacted by agricultural land use should be restored and that the least-impacted forest sites could serve as reference conditions. 相似文献
236.
Fabien Vincent Alejandra Acevedo Margaret T. Nguyen Michelle Dourado Jeff DeFalco Amy Gustafson Peter Spiro Daniel E. Emerling Michael G. Kelly Matthew A.J. Duncton 《Biochemical and biophysical research communications》2009,389(3):490-494
TRPV4, a close relative of the vanilloid receptor TRPV1, is activated by diverse modalities such as endogenous lipid ligands, hypotonicity, protein kinases and, possibly, mechanical inputs. While its multiple roles in vivo are being explored with KO mice and selective agonists, there is a dearth of selective antagonists available to examine TRPV4 function. Herein we detail the use of a focused library of commercial compounds in order to identify RN-1747 and RN-1734, a pair of structurally related small molecules endowed with TRPV4 agonist and antagonist properties, respectively. Their activities against human, rat and mouse TRPV4 were characterized using electrophysiology and intracellular calcium influx. Significantly, antagonist RN-1734 was observed to completely inhibit both ligand- and hypotonicity-activated TRPV4. In addition, RN-1734 was found to be selective for TRPV4 in a TRP selectivity panel including TRPV1, TRPV3 and TRPM8, and could thus be a valuable pharmacological probe for TRPV4 studies. 相似文献
237.
HPL-2/HP1 Prevents Inappropriate Vulval Induction in Caenorhabditis elegans by Acting in Both HYP7 and Vulval Precursor Cells
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A current model for Caenorhabditis elegans vulval cell fate specification is that SynMuv genes act redundantly in the hyp7 hypodermal syncytium to repress the LIN-3/EGF inducer and prevent ectopic vulval induction of vulva precursor cells (VPCs). Here we show that the SynMuv gene hpl-2/HP1 has an additional function in VPCs, where it may act through target genes including LIN-39/Hox. 相似文献
238.
Fabien Knoll 《Geobios》2009,42(6):755
The remains of an ornithopod dinosaur from the Upper Barremian (Lower Cretaceous) of Auxerre (Burgundy, France) are described. They consist of several vertebrae and a fragmentary scapula. Despite obvious morphological and dimensional affinities with the species Iguanodon bernissartensis, the state of the specimen does not allow any identification more precise than Iguanodontia indet. Reappraisal of the French Upper Barremian record of medium and large-sized ornithopods reveals that no specimen can be definitely determined to the specific level. 相似文献
239.
Sara Romano Fabien Aujoulat Estelle Jumas-Bilak Agnès Masnou Jean-Luc Jeannot Enevold Falsen Hélène Marchandin Corinne Teyssier 《BMC microbiology》2009,9(1):267
Background
Ochrobactrum anthropi is a versatile bacterial species with strains living in very diverse habitats. It is increasingly recognized as opportunistic pathogen in hospitalized patients. The population biology of the species particularly with regard to the characteristics of the human isolates is being investigated. To address this issue, we proposed a polyphasic approach consisting in Multi-Locus Sequence Typing (MLST), multi-locus phylogeny, genomic-based fingerprinting by pulsed-field gel electrophoresis (PFGE) and antibiotyping. 相似文献240.
Sylvie Rodrigues-Ferreira Anne Di Tommaso Ariane Dimitrov Sylvie Cazaubon Nadège Gruel Hélène Colasson André Nicolas Nathalie Chaverot Vincent Molinié Fabien Reyal Brigitte Sigal-Zafrani Benoit Terris Olivier Delattre Fran?ois Radvanyi Franck Perez Anne Vincent-Salomon Clara Nahmias 《PloS one》2009,4(10)