全文获取类型
收费全文 | 1203篇 |
免费 | 130篇 |
出版年
2024年 | 1篇 |
2023年 | 10篇 |
2022年 | 20篇 |
2021年 | 31篇 |
2020年 | 25篇 |
2019年 | 27篇 |
2018年 | 24篇 |
2017年 | 41篇 |
2016年 | 46篇 |
2015年 | 81篇 |
2014年 | 84篇 |
2013年 | 87篇 |
2012年 | 124篇 |
2011年 | 99篇 |
2010年 | 68篇 |
2009年 | 78篇 |
2008年 | 86篇 |
2007年 | 97篇 |
2006年 | 62篇 |
2005年 | 56篇 |
2004年 | 57篇 |
2003年 | 33篇 |
2002年 | 46篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 7篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1984年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有1333条查询结果,搜索用时 31 毫秒
991.
992.
993.
Katharina Nakel Fabien Bonneau Claire Basquin Bianca Habermann Christian R. Eckmann Elena Conti 《RNA (New York, N.Y.)》2016,22(8):1139-1145
Cytoplasmic polyadenylation drives the translational activation of specific mRNAs in early metazoan development and is performed by distinct complexes that share the same catalytic poly(A)-polymerase subunit, GLD-2. The activity and specificity of GLD-2 depend on its binding partners. In Caenorhabditis elegans, GLD-2 promotes spermatogenesis when bound to GLD-3 and oogenesis when bound to RNP-8. GLD-3 and RNP-8 antagonize each other and compete for GLD-2 binding. Following up on our previous mechanistic studies of GLD-2–GLD-3, we report here the 2.5 Å resolution structure and biochemical characterization of a GLD-2–RNP-8 core complex. In the structure, RNP-8 embraces the poly(A)-polymerase, docking onto several conserved hydrophobic hotspots present on the GLD-2 surface. RNP-8 stabilizes GLD-2 and indirectly stimulates polyadenylation. RNP-8 has a different amino-acid sequence and structure as compared to GLD-3. Yet, it binds the same surfaces of GLD-2 by forming alternative interactions, rationalizing the remarkable versatility of GLD-2 complexes. 相似文献
994.
Functional Characterization of a Nup159p-containing Nuclear Pore Subcomplex 总被引:11,自引:3,他引:8
下载免费PDF全文
![点击此处可从《Molecular biology of the cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Naïma Belgareh Christine Snay-Hodge Fabien Pasteau Suzanne Dagher Charles N. Cole Valrie Doye 《Molecular biology of the cell》1998,9(12):3475-3492
Nup159p/Rat7p is an essential FG repeat–containing nucleoporin localized at the cytoplasmic face of the nuclear pore complex (NPC) and involved in poly(A)+ RNA export and NPC distribution. A detailed structural–functional analysis of this nucleoporin previously demonstrated that Nup159p is anchored within the NPC through its essential carboxyl-terminal domain. In this study, we demonstrate that Nup159p specifically interacts through this domain with both Nsp1p and Nup82p. Further analysis of the interactions within the Nup159p/Nsp1p/Nup82p subcomplex using the nup82Δ108 mutant strain revealed that a deletion within the carboxyl-terminal domain of Nup82p prevents its interaction with Nsp1p but does not affect the interaction between Nup159p and Nsp1p. Moreover, immunofluorescence analysis demonstrated that Nup159p is delocalized from the NPC in nup82Δ108 cells grown at 37°C, a temperature at which the Nup82Δ108p mutant protein becomes degraded. This suggests that Nup82p may act as a docking site for a core complex composed of the repeat-containing nucleoporins Nup159p and Nsp1p. In vivo transport assays further revealed that nup82Δ108 and nup159-1/rat7-1 mutant strains have little if any defect in nuclear protein import and protein export. Together our data suggest that the poly(A)+ RNA export defect previously observed in nup82 mutant cells might be due to the loss from the NPCs of the repeat-containing nucleoporin Nup159p. 相似文献
995.
Differential Utilization of Ras Signaling Pathways by Macrophage Colony-Stimulating Factor (CSF) and Granulocyte-Macrophage CSF Receptors during Macrophage Differentiation 总被引:3,自引:1,他引:2
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
996.
997.
Florent Levavasseur Patrice K. Kouakou Julie Constantin Romain Cresson Fabien Ferchaud Romain Girault Vincent Jean-Baptiste Hélène Lagrange Sylvain Marsac Sylvain Pellerin Sabine Houot 《Global Change Biology Bioenergy》2023,15(2):224-238
Energy cover crops for biogas production through anaerobic digestion (AD) are inserted between two primary crops. They replace either bare soil or nonharvested cover crops, and their management is usually intensified to produce more biomass. They allow the production of renewable energy as well as digestate, used as an organic fertilizer, without directly competing with food production. Because of the increased biomass production and export and of the return of a digested biomass to the soil, the impact of energy cover crops on soil organic carbon (SOC) is questioned. The objective of this paper was to study the difference in SOC stocks induced by the introduction of energy cover crops for AD coupled with the application of the resulting amount of digestate. We used the AD model Sys-Metha combined with the soil C model AMG to simulate SOC stocks for 13 case studies in France, with scenarios comparing different intercrop management practices, with or without cover crops, harvested or not. Our results indicated that the higher biomass production of energy cover crops (from 6.7 to 11.1 t DM ha−1) in comparison with nonharvested cover crops (2 t DM ha−1) or bare soil led to higher humified C input (belowground input and digestate), despite the high C fraction exported in AD. This resulted in an increase in SOC stocks in comparison with nonharvested cover crops or bare soil (from 0.01 to 0.12 t C ha−1 year−1 over 30 years). The uncertainties in the model parameters did not modify these results. However, in the case of equal biomass production between energy cover crops and nonharvested cover crops, SOC stocks would be lower with energy cover crops. 相似文献
998.
C Paulin N Fabien A Fusco M P Pages M C Patricot H Bornet P M Dubois 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》1992,315(12):493-498
One of the difficulties in characterization of the oncogenes involved in thyroid carcinogenesis is the production of cell lines. Arising from a poorly differentiated thyroid papillary carcinoma we have established a cell line synthesizing the thyroglobulin and human chorionic gonadotropin (alpha and beta subunits) (HCG) hormones. These cells will allow research of the oncogenes involved or potentially involved in thyroid papillary carcinomas and evaluation of the role of the autocrine secretion of HCG. 相似文献
999.
1000.
E2F1 interacts with BCL‐xL and regulates its subcellular localization dynamics to trigger cell death
下载免费PDF全文
![点击此处可从《EMBO reports》网站下载免费的PDF全文](/ch/ext_images/free.gif)