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391.
Fabiano de A. Ximenes Fabiane Quirino de Paula Silveira Edivaldo Ximenes F. Filho 《Current microbiology》1996,33(2):71-77
Nine Trichoderma harzianum strains were screened for β-xylosidase activity when grown in solid-state cultures on media containing wheat bran as the
carbon source. All strains produced β-xylosidase activity, the most active being in extracts of cultures of T. harzianum strain 4. A β-xylosidase was purified by ammonium sulfate precipitation, ultrafiltration, gel filtration, and ion exchange
chromatography from solid-state cultures of T. harzianum strain C. Enzyme preparations yielded a single band when stained for protein following eletrophoresis. The molecular weight
value, calculated following SDS-PAGE, was determined to be 60 kDa. β-Xylosidase was most active at pH 4.0–4.5 and 70°C. This
enzyme had a K
m
value of 0.053 mM. The phenol-sulfuric acid method detected the presence of a small amount of carbohydrate in the purified
enzyme preparation. β-Xylosidase was active against some p-nitrophenylglycosides. The enzyme was inactive against xylan and PNPG. β-xylosidase activity was inhibited by xylose and
SDS. Iodoacetamide, dithiothreitol, gluconolactone, glucose, and mercuric chloride failed to inactivate this enzyme's activity.
A synergistic effect was observed when β-xylosidase from T. harzianum strain C and β-xylanase from Aspergillus fumigatus were incubated with pretreated arabinoxylan.
Received: 1 December 1995 / Accepted: 11 December 1995 相似文献
392.